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Co-Production of Cellulose Nanocrystals and Fermentable Sugars Assisted by Endoglucanase Treatment of Wood Pulp
In this study, fermentable sugars and cellulose nanocrystals (CNCs) were co-produced from endoglucanase treatment of wood pulp, followed by acid hydrolysis. Enzymatic hydrolysis was performed using two endoglucanases differentiated by the presence or absence of a cellulose-binding domain (CBD). The...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165468/ https://www.ncbi.nlm.nih.gov/pubmed/30205440 http://dx.doi.org/10.3390/ma11091645 |
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author | Dai, Jing Chae, Michael Beyene, Dawit Danumah, Christophe Tosto, Frank Bressler, David C. |
author_facet | Dai, Jing Chae, Michael Beyene, Dawit Danumah, Christophe Tosto, Frank Bressler, David C. |
author_sort | Dai, Jing |
collection | PubMed |
description | In this study, fermentable sugars and cellulose nanocrystals (CNCs) were co-produced from endoglucanase treatment of wood pulp, followed by acid hydrolysis. Enzymatic hydrolysis was performed using two endoglucanases differentiated by the presence or absence of a cellulose-binding domain (CBD). The enzyme with an intact CBD gave the higher glucan conversion (up to 14.1 ± 1.2 wt %) and improved the degree of crystallinity of the recovered wood pulp fiber (up to 83.0 ± 1.0%). Thus, this endoglucanase-assisted treatment successfully removed amorphous content from the original cellulosic feedstock. CNC recovery (16.9 ± 0.7 wt %) from the feedstock going into the acid hydrolysis was improved relative to untreated pulp (13.2 ± 0.6 wt %). The mass loss from enzymatic treatment did not cause a decrease in the CNC yield from the starting material. The characteristics of CNCs obtained through acid hydrolysis (with or without enzyme treatment of pulp) were analyzed using X-ray diffraction, transmission electron microscopy, dynamic light scattering, Fourier transform infrared spectroscopy, and differential scanning calorimetry as characterization techniques. The CNCs generated through acid hydrolysis of endoglucanase-treated wood pulp displayed comparable properties relative to those generated using untreated pulp. Thus, endoglucanase treatment can enable co-production of CNCs and sugars for biofuel fermentation. |
format | Online Article Text |
id | pubmed-6165468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61654682018-10-12 Co-Production of Cellulose Nanocrystals and Fermentable Sugars Assisted by Endoglucanase Treatment of Wood Pulp Dai, Jing Chae, Michael Beyene, Dawit Danumah, Christophe Tosto, Frank Bressler, David C. Materials (Basel) Article In this study, fermentable sugars and cellulose nanocrystals (CNCs) were co-produced from endoglucanase treatment of wood pulp, followed by acid hydrolysis. Enzymatic hydrolysis was performed using two endoglucanases differentiated by the presence or absence of a cellulose-binding domain (CBD). The enzyme with an intact CBD gave the higher glucan conversion (up to 14.1 ± 1.2 wt %) and improved the degree of crystallinity of the recovered wood pulp fiber (up to 83.0 ± 1.0%). Thus, this endoglucanase-assisted treatment successfully removed amorphous content from the original cellulosic feedstock. CNC recovery (16.9 ± 0.7 wt %) from the feedstock going into the acid hydrolysis was improved relative to untreated pulp (13.2 ± 0.6 wt %). The mass loss from enzymatic treatment did not cause a decrease in the CNC yield from the starting material. The characteristics of CNCs obtained through acid hydrolysis (with or without enzyme treatment of pulp) were analyzed using X-ray diffraction, transmission electron microscopy, dynamic light scattering, Fourier transform infrared spectroscopy, and differential scanning calorimetry as characterization techniques. The CNCs generated through acid hydrolysis of endoglucanase-treated wood pulp displayed comparable properties relative to those generated using untreated pulp. Thus, endoglucanase treatment can enable co-production of CNCs and sugars for biofuel fermentation. MDPI 2018-09-07 /pmc/articles/PMC6165468/ /pubmed/30205440 http://dx.doi.org/10.3390/ma11091645 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dai, Jing Chae, Michael Beyene, Dawit Danumah, Christophe Tosto, Frank Bressler, David C. Co-Production of Cellulose Nanocrystals and Fermentable Sugars Assisted by Endoglucanase Treatment of Wood Pulp |
title | Co-Production of Cellulose Nanocrystals and Fermentable Sugars Assisted by Endoglucanase Treatment of Wood Pulp |
title_full | Co-Production of Cellulose Nanocrystals and Fermentable Sugars Assisted by Endoglucanase Treatment of Wood Pulp |
title_fullStr | Co-Production of Cellulose Nanocrystals and Fermentable Sugars Assisted by Endoglucanase Treatment of Wood Pulp |
title_full_unstemmed | Co-Production of Cellulose Nanocrystals and Fermentable Sugars Assisted by Endoglucanase Treatment of Wood Pulp |
title_short | Co-Production of Cellulose Nanocrystals and Fermentable Sugars Assisted by Endoglucanase Treatment of Wood Pulp |
title_sort | co-production of cellulose nanocrystals and fermentable sugars assisted by endoglucanase treatment of wood pulp |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165468/ https://www.ncbi.nlm.nih.gov/pubmed/30205440 http://dx.doi.org/10.3390/ma11091645 |
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