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Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions—Ara h2 and Ara h6 Are Readily Bio-Accessible

The oral mucosa is the first immune tissue that encounters allergens upon ingestion of food. We hypothesized that the bio-accessibility of allergens at this stage may be a key determinant for sensitization. Light roasted peanut flour was suspended at various pH in buffers mimicking saliva. Protein c...

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Autores principales: Koppelman, Stef J., Smits, Mieke, Tomassen, Monic, de Jong, Govardus A.H., Baumert, Joe, Taylor, Steve L., Witkamp, Renger, Veldman, Robert Jan, Pieters, Raymond, Wichers, Harry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165493/
https://www.ncbi.nlm.nih.gov/pubmed/30208580
http://dx.doi.org/10.3390/nu10091281
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author Koppelman, Stef J.
Smits, Mieke
Tomassen, Monic
de Jong, Govardus A.H.
Baumert, Joe
Taylor, Steve L.
Witkamp, Renger
Veldman, Robert Jan
Pieters, Raymond
Wichers, Harry
author_facet Koppelman, Stef J.
Smits, Mieke
Tomassen, Monic
de Jong, Govardus A.H.
Baumert, Joe
Taylor, Steve L.
Witkamp, Renger
Veldman, Robert Jan
Pieters, Raymond
Wichers, Harry
author_sort Koppelman, Stef J.
collection PubMed
description The oral mucosa is the first immune tissue that encounters allergens upon ingestion of food. We hypothesized that the bio-accessibility of allergens at this stage may be a key determinant for sensitization. Light roasted peanut flour was suspended at various pH in buffers mimicking saliva. Protein concentrations and allergens profiles were determined in the supernatants. Peanut protein solubility was poor in the pH range between 3 and 6, while at a low pH (1.5) and at moderately high pHs (>8), it increased. In the pH range of saliva, between 6.5 and 8.5, the allergens Ara h2 and Ara h6 were readily released, whereas Ara h1 and Ara h3 were poorly released. Increasing the pH from 6.5 to 8.5 slightly increased the release of Ara h1 and Ara h3, but the recovery remained low (approximately 20%) compared to that of Ara h2 and Ara h6 (approximately 100% and 65%, respectively). This remarkable difference in the extraction kinetics suggests that Ara h2 and Ara h6 are the first allergens an individual is exposed to upon ingestion of peanut-containing food. We conclude that the peanut allergens Ara h2 and Ara h6 are quickly bio-accessible in the mouth, potentially explaining their extraordinary allergenicity.
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spelling pubmed-61654932018-10-10 Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions—Ara h2 and Ara h6 Are Readily Bio-Accessible Koppelman, Stef J. Smits, Mieke Tomassen, Monic de Jong, Govardus A.H. Baumert, Joe Taylor, Steve L. Witkamp, Renger Veldman, Robert Jan Pieters, Raymond Wichers, Harry Nutrients Article The oral mucosa is the first immune tissue that encounters allergens upon ingestion of food. We hypothesized that the bio-accessibility of allergens at this stage may be a key determinant for sensitization. Light roasted peanut flour was suspended at various pH in buffers mimicking saliva. Protein concentrations and allergens profiles were determined in the supernatants. Peanut protein solubility was poor in the pH range between 3 and 6, while at a low pH (1.5) and at moderately high pHs (>8), it increased. In the pH range of saliva, between 6.5 and 8.5, the allergens Ara h2 and Ara h6 were readily released, whereas Ara h1 and Ara h3 were poorly released. Increasing the pH from 6.5 to 8.5 slightly increased the release of Ara h1 and Ara h3, but the recovery remained low (approximately 20%) compared to that of Ara h2 and Ara h6 (approximately 100% and 65%, respectively). This remarkable difference in the extraction kinetics suggests that Ara h2 and Ara h6 are the first allergens an individual is exposed to upon ingestion of peanut-containing food. We conclude that the peanut allergens Ara h2 and Ara h6 are quickly bio-accessible in the mouth, potentially explaining their extraordinary allergenicity. MDPI 2018-09-11 /pmc/articles/PMC6165493/ /pubmed/30208580 http://dx.doi.org/10.3390/nu10091281 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Koppelman, Stef J.
Smits, Mieke
Tomassen, Monic
de Jong, Govardus A.H.
Baumert, Joe
Taylor, Steve L.
Witkamp, Renger
Veldman, Robert Jan
Pieters, Raymond
Wichers, Harry
Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions—Ara h2 and Ara h6 Are Readily Bio-Accessible
title Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions—Ara h2 and Ara h6 Are Readily Bio-Accessible
title_full Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions—Ara h2 and Ara h6 Are Readily Bio-Accessible
title_fullStr Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions—Ara h2 and Ara h6 Are Readily Bio-Accessible
title_full_unstemmed Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions—Ara h2 and Ara h6 Are Readily Bio-Accessible
title_short Release of Major Peanut Allergens from Their Matrix under Various pH and Simulated Saliva Conditions—Ara h2 and Ara h6 Are Readily Bio-Accessible
title_sort release of major peanut allergens from their matrix under various ph and simulated saliva conditions—ara h2 and ara h6 are readily bio-accessible
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165493/
https://www.ncbi.nlm.nih.gov/pubmed/30208580
http://dx.doi.org/10.3390/nu10091281
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