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A Disposable and Multi-Chamber Film-Based PCR Chip for Detection of Foodborne Pathogen

Since the increment of the threat to public health caused by foodborne pathogens, researches have been widely studied on developing the miniaturized detection system for the on-site pathogen detection. In the study, we focused on the development of portable, robust, and disposable film-based polymer...

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Autores principales: Bae, Nam Ho, Lim, Sun Young, Song, Younseong, Jeong, Soon Woo, Shin, Seol Yi, Kim, Yong Tae, Lee, Tae Jae, Lee, Kyoung G., Lee, Seok Jae, Oh, Yong-Jun, Park, Yoo Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165562/
https://www.ncbi.nlm.nih.gov/pubmed/30235826
http://dx.doi.org/10.3390/s18093158
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author Bae, Nam Ho
Lim, Sun Young
Song, Younseong
Jeong, Soon Woo
Shin, Seol Yi
Kim, Yong Tae
Lee, Tae Jae
Lee, Kyoung G.
Lee, Seok Jae
Oh, Yong-Jun
Park, Yoo Min
author_facet Bae, Nam Ho
Lim, Sun Young
Song, Younseong
Jeong, Soon Woo
Shin, Seol Yi
Kim, Yong Tae
Lee, Tae Jae
Lee, Kyoung G.
Lee, Seok Jae
Oh, Yong-Jun
Park, Yoo Min
author_sort Bae, Nam Ho
collection PubMed
description Since the increment of the threat to public health caused by foodborne pathogens, researches have been widely studied on developing the miniaturized detection system for the on-site pathogen detection. In the study, we focused on the development of portable, robust, and disposable film-based polymerase chain reaction (PCR) chip containing a multiplex chamber for simultaneous gene amplification. In order to simply fabricate and operate a film-based PCR chip, different kinds of PCR chambers were designed and fabricated using polyethylene terephthalate (PET) and polyvinyl chloride (PVC) adhesive film, in comparison with commercial PCR, which employs a stereotyped system at a bench-top scale. No reagent leakage was confirmed during the PCR thermal cycling using the film PCR chip, which indicates that the film PCR chip is structurally stable for rapid heat cycling for DNA amplification. Owing to use of the thin film to fabricate the PCR chip, we are able to realize fast thermal transfer from the heat block that leads to short PCR amplification time. Moreover, using the film PCR chip, we could even amplify the target pathogen with 10 CFU mL(−1). The artificially infected milk with various concentration of Bacillus cereus was successfully amplified on a single film PCR chip. On the basis of the reliable results, the developed film PCR chip could be a useful tool as a POCT device to detect foodborne pathogens via genetic analysis.
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spelling pubmed-61655622018-10-10 A Disposable and Multi-Chamber Film-Based PCR Chip for Detection of Foodborne Pathogen Bae, Nam Ho Lim, Sun Young Song, Younseong Jeong, Soon Woo Shin, Seol Yi Kim, Yong Tae Lee, Tae Jae Lee, Kyoung G. Lee, Seok Jae Oh, Yong-Jun Park, Yoo Min Sensors (Basel) Article Since the increment of the threat to public health caused by foodborne pathogens, researches have been widely studied on developing the miniaturized detection system for the on-site pathogen detection. In the study, we focused on the development of portable, robust, and disposable film-based polymerase chain reaction (PCR) chip containing a multiplex chamber for simultaneous gene amplification. In order to simply fabricate and operate a film-based PCR chip, different kinds of PCR chambers were designed and fabricated using polyethylene terephthalate (PET) and polyvinyl chloride (PVC) adhesive film, in comparison with commercial PCR, which employs a stereotyped system at a bench-top scale. No reagent leakage was confirmed during the PCR thermal cycling using the film PCR chip, which indicates that the film PCR chip is structurally stable for rapid heat cycling for DNA amplification. Owing to use of the thin film to fabricate the PCR chip, we are able to realize fast thermal transfer from the heat block that leads to short PCR amplification time. Moreover, using the film PCR chip, we could even amplify the target pathogen with 10 CFU mL(−1). The artificially infected milk with various concentration of Bacillus cereus was successfully amplified on a single film PCR chip. On the basis of the reliable results, the developed film PCR chip could be a useful tool as a POCT device to detect foodborne pathogens via genetic analysis. MDPI 2018-09-19 /pmc/articles/PMC6165562/ /pubmed/30235826 http://dx.doi.org/10.3390/s18093158 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bae, Nam Ho
Lim, Sun Young
Song, Younseong
Jeong, Soon Woo
Shin, Seol Yi
Kim, Yong Tae
Lee, Tae Jae
Lee, Kyoung G.
Lee, Seok Jae
Oh, Yong-Jun
Park, Yoo Min
A Disposable and Multi-Chamber Film-Based PCR Chip for Detection of Foodborne Pathogen
title A Disposable and Multi-Chamber Film-Based PCR Chip for Detection of Foodborne Pathogen
title_full A Disposable and Multi-Chamber Film-Based PCR Chip for Detection of Foodborne Pathogen
title_fullStr A Disposable and Multi-Chamber Film-Based PCR Chip for Detection of Foodborne Pathogen
title_full_unstemmed A Disposable and Multi-Chamber Film-Based PCR Chip for Detection of Foodborne Pathogen
title_short A Disposable and Multi-Chamber Film-Based PCR Chip for Detection of Foodborne Pathogen
title_sort disposable and multi-chamber film-based pcr chip for detection of foodborne pathogen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165562/
https://www.ncbi.nlm.nih.gov/pubmed/30235826
http://dx.doi.org/10.3390/s18093158
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