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Variability of defensin genes from a Mexican endemic Triatominae: Triatoma (Meccus) pallidipennis (Hemiptera: Reduviidae)

Chagas disease remains a serious health problem for countries where the most common mode of transmission is infection contracted from the feces of a Triatominae insect vector. In México, 32 species of Triatoma have been identified; amongst them, Triatoma (Meccus) pallidipennis is an endemic species...

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Autores principales: Díaz-Garrido, Paulina, Sepúlveda-Robles, Omar, Martínez-Martínez, Ignacio, Espinoza, Bertha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165835/
https://www.ncbi.nlm.nih.gov/pubmed/30181380
http://dx.doi.org/10.1042/BSR20180988
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author Díaz-Garrido, Paulina
Sepúlveda-Robles, Omar
Martínez-Martínez, Ignacio
Espinoza, Bertha
author_facet Díaz-Garrido, Paulina
Sepúlveda-Robles, Omar
Martínez-Martínez, Ignacio
Espinoza, Bertha
author_sort Díaz-Garrido, Paulina
collection PubMed
description Chagas disease remains a serious health problem for countries where the most common mode of transmission is infection contracted from the feces of a Triatominae insect vector. In México, 32 species of Triatoma have been identified; amongst them, Triatoma (Meccus) pallidipennis is an endemic species reported to have high percentages of infection with T. cruzi. Defensins, cysteine-rich cationic peptides, are a family of antimicrobial peptides (AMPs); the synthesis of these molecules is crucial for insect’s immune defense. In the present study, the genes encoding defensins in T. pallidipennis were sequenced with the purpose of identifying the variability of these genes in a Mexican vector of T. cruzi. We found 12 different genes encoding three mature peptides, all of which had the typical folding of a functional insect defensin. In this work two Defensins type 1 and one type 4 were identified. The pro-peptide domain was highly variable and the mature peptide was not. This is the first report focus on variability of defensins from an epidemiologically important Triatoma in Mexico.
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spelling pubmed-61658352018-10-04 Variability of defensin genes from a Mexican endemic Triatominae: Triatoma (Meccus) pallidipennis (Hemiptera: Reduviidae) Díaz-Garrido, Paulina Sepúlveda-Robles, Omar Martínez-Martínez, Ignacio Espinoza, Bertha Biosci Rep Research Articles Chagas disease remains a serious health problem for countries where the most common mode of transmission is infection contracted from the feces of a Triatominae insect vector. In México, 32 species of Triatoma have been identified; amongst them, Triatoma (Meccus) pallidipennis is an endemic species reported to have high percentages of infection with T. cruzi. Defensins, cysteine-rich cationic peptides, are a family of antimicrobial peptides (AMPs); the synthesis of these molecules is crucial for insect’s immune defense. In the present study, the genes encoding defensins in T. pallidipennis were sequenced with the purpose of identifying the variability of these genes in a Mexican vector of T. cruzi. We found 12 different genes encoding three mature peptides, all of which had the typical folding of a functional insect defensin. In this work two Defensins type 1 and one type 4 were identified. The pro-peptide domain was highly variable and the mature peptide was not. This is the first report focus on variability of defensins from an epidemiologically important Triatoma in Mexico. Portland Press Ltd. 2018-09-28 /pmc/articles/PMC6165835/ /pubmed/30181380 http://dx.doi.org/10.1042/BSR20180988 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Díaz-Garrido, Paulina
Sepúlveda-Robles, Omar
Martínez-Martínez, Ignacio
Espinoza, Bertha
Variability of defensin genes from a Mexican endemic Triatominae: Triatoma (Meccus) pallidipennis (Hemiptera: Reduviidae)
title Variability of defensin genes from a Mexican endemic Triatominae: Triatoma (Meccus) pallidipennis (Hemiptera: Reduviidae)
title_full Variability of defensin genes from a Mexican endemic Triatominae: Triatoma (Meccus) pallidipennis (Hemiptera: Reduviidae)
title_fullStr Variability of defensin genes from a Mexican endemic Triatominae: Triatoma (Meccus) pallidipennis (Hemiptera: Reduviidae)
title_full_unstemmed Variability of defensin genes from a Mexican endemic Triatominae: Triatoma (Meccus) pallidipennis (Hemiptera: Reduviidae)
title_short Variability of defensin genes from a Mexican endemic Triatominae: Triatoma (Meccus) pallidipennis (Hemiptera: Reduviidae)
title_sort variability of defensin genes from a mexican endemic triatominae: triatoma (meccus) pallidipennis (hemiptera: reduviidae)
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165835/
https://www.ncbi.nlm.nih.gov/pubmed/30181380
http://dx.doi.org/10.1042/BSR20180988
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