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d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission

d Rhamnose β-hederin (DRβ-H), an active component extracted from the traditional Chinese medicinal plant Clematis ganpiniana, has been reported to be effective against breast cancer. Recent studies have also indicated that the isolated exosomes (D/exo) from docetaxel-resistant breast cancer cells MC...

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Autores principales: Chen, Wei-xian, Xu, Ling-yun, Qian, Qi, He, Xiao, Peng, Wen-ting, Fan, Wen-qiang, Zhu, Yu-lan, Tang, Jin-hai, Cheng, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165836/
https://www.ncbi.nlm.nih.gov/pubmed/30061173
http://dx.doi.org/10.1042/BSR20180110
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author Chen, Wei-xian
Xu, Ling-yun
Qian, Qi
He, Xiao
Peng, Wen-ting
Fan, Wen-qiang
Zhu, Yu-lan
Tang, Jin-hai
Cheng, Lin
author_facet Chen, Wei-xian
Xu, Ling-yun
Qian, Qi
He, Xiao
Peng, Wen-ting
Fan, Wen-qiang
Zhu, Yu-lan
Tang, Jin-hai
Cheng, Lin
author_sort Chen, Wei-xian
collection PubMed
description d Rhamnose β-hederin (DRβ-H), an active component extracted from the traditional Chinese medicinal plant Clematis ganpiniana, has been reported to be effective against breast cancer. Recent studies have also indicated that the isolated exosomes (D/exo) from docetaxel-resistant breast cancer cells MCF-7 (MCF-7/Doc) were associated with resistance transmission by delivering genetic cargo. However, the relevance of D/exo during DRβ-H exposure remains largely unclear. In the present work, exosomes were characterized by morphology and size distribution. We reinforced the significant role of D/exo in spreading chemoresistance from MCF-7/Doc to recipient sensitive cells after absorption and internalization. DRβ-H could reduce the formation and release of D/exo. Next, we demonstrated that DRβ-H was able to reverse docetaxel resistance and that D/exo was responsible for DRβ-H-mediated resistance reversal. We also found that DRβ-H could decrease the expressions of several most abundant miRNAs (miR-16, miR-23a, miR-24, miR-26a, and miR-27a) transported by D/exo. Target gene prediction and pathway analysis showed the involvement of these selected miRNAs in pathways related to treatment failure. Our results suggested that DRβ-H could reduce D/exo secretion from MCF-7/Doc cells and induce the reduction in resistance transmission via D/exo.
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spelling pubmed-61658362018-10-04 d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission Chen, Wei-xian Xu, Ling-yun Qian, Qi He, Xiao Peng, Wen-ting Fan, Wen-qiang Zhu, Yu-lan Tang, Jin-hai Cheng, Lin Biosci Rep Research Articles d Rhamnose β-hederin (DRβ-H), an active component extracted from the traditional Chinese medicinal plant Clematis ganpiniana, has been reported to be effective against breast cancer. Recent studies have also indicated that the isolated exosomes (D/exo) from docetaxel-resistant breast cancer cells MCF-7 (MCF-7/Doc) were associated with resistance transmission by delivering genetic cargo. However, the relevance of D/exo during DRβ-H exposure remains largely unclear. In the present work, exosomes were characterized by morphology and size distribution. We reinforced the significant role of D/exo in spreading chemoresistance from MCF-7/Doc to recipient sensitive cells after absorption and internalization. DRβ-H could reduce the formation and release of D/exo. Next, we demonstrated that DRβ-H was able to reverse docetaxel resistance and that D/exo was responsible for DRβ-H-mediated resistance reversal. We also found that DRβ-H could decrease the expressions of several most abundant miRNAs (miR-16, miR-23a, miR-24, miR-26a, and miR-27a) transported by D/exo. Target gene prediction and pathway analysis showed the involvement of these selected miRNAs in pathways related to treatment failure. Our results suggested that DRβ-H could reduce D/exo secretion from MCF-7/Doc cells and induce the reduction in resistance transmission via D/exo. Portland Press Ltd. 2018-09-28 /pmc/articles/PMC6165836/ /pubmed/30061173 http://dx.doi.org/10.1042/BSR20180110 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Chen, Wei-xian
Xu, Ling-yun
Qian, Qi
He, Xiao
Peng, Wen-ting
Fan, Wen-qiang
Zhu, Yu-lan
Tang, Jin-hai
Cheng, Lin
d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
title d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
title_full d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
title_fullStr d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
title_full_unstemmed d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
title_short d Rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
title_sort d rhamnose β-hederin reverses chemoresistance of breast cancer cells by regulating exosome-mediated resistance transmission
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165836/
https://www.ncbi.nlm.nih.gov/pubmed/30061173
http://dx.doi.org/10.1042/BSR20180110
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