Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Methylotrophic yeasts have widely been used as model organisms for understanding cellular functions and biochemical activities in lower eukaryotes. The gene encoding an aspartic protease (MCAP) from Mucor circinelloides DSM 2183 was cloned and expressed into Pichia pastoris using both the native M....

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Detalles Bibliográficos
Autores principales: Kangwa, Martin, Salgado, Jose Antonio Gama, Fernandez-Lahore, Hector Marcelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167268/
https://www.ncbi.nlm.nih.gov/pubmed/30276572
http://dx.doi.org/10.1186/s13568-018-0691-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167268/
https://www.ncbi.nlm.nih.gov/pubmed/30276572
http://dx.doi.org/10.1186/s13568-018-0691-3

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