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CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering

Microbes use diverse defence strategies that allow them to withstand exposure to a variety of genome invaders such as bacteriophages and plasmids. One such defence strategy is the use of RNA guided endonuclease called CRISPR-associated (Cas) 9 protein. The Cas9 protein, derived from type II CRISPR/C...

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Autores principales: Lone, Bilal Ahmad, Karna, Shibendra Kumar Lal, Ahmad, Faiz, Shahi, Nerina, Pokharel, Yuba Raj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167567/
https://www.ncbi.nlm.nih.gov/pubmed/30319822
http://dx.doi.org/10.1155/2018/3797214
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author Lone, Bilal Ahmad
Karna, Shibendra Kumar Lal
Ahmad, Faiz
Shahi, Nerina
Pokharel, Yuba Raj
author_facet Lone, Bilal Ahmad
Karna, Shibendra Kumar Lal
Ahmad, Faiz
Shahi, Nerina
Pokharel, Yuba Raj
author_sort Lone, Bilal Ahmad
collection PubMed
description Microbes use diverse defence strategies that allow them to withstand exposure to a variety of genome invaders such as bacteriophages and plasmids. One such defence strategy is the use of RNA guided endonuclease called CRISPR-associated (Cas) 9 protein. The Cas9 protein, derived from type II CRISPR/Cas system, has been adapted as a versatile tool for genome targeting and engineering due to its simplicity and high efficiency over the earlier tools such as ZFNs and TALENs. With recent advancements, CRISPR/Cas9 technology has emerged as a revolutionary tool for modulating the genome in living cells and inspires innovative translational applications in different fields. In this paper we review the developments and its potential uses in the CRISPR/Cas9 technology as well as recent advancements in genome engineering using CRISPR/Cas9.
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spelling pubmed-61675672018-10-14 CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering Lone, Bilal Ahmad Karna, Shibendra Kumar Lal Ahmad, Faiz Shahi, Nerina Pokharel, Yuba Raj Genet Res Int Review Article Microbes use diverse defence strategies that allow them to withstand exposure to a variety of genome invaders such as bacteriophages and plasmids. One such defence strategy is the use of RNA guided endonuclease called CRISPR-associated (Cas) 9 protein. The Cas9 protein, derived from type II CRISPR/Cas system, has been adapted as a versatile tool for genome targeting and engineering due to its simplicity and high efficiency over the earlier tools such as ZFNs and TALENs. With recent advancements, CRISPR/Cas9 technology has emerged as a revolutionary tool for modulating the genome in living cells and inspires innovative translational applications in different fields. In this paper we review the developments and its potential uses in the CRISPR/Cas9 technology as well as recent advancements in genome engineering using CRISPR/Cas9. Hindawi 2018-09-18 /pmc/articles/PMC6167567/ /pubmed/30319822 http://dx.doi.org/10.1155/2018/3797214 Text en Copyright © 2018 Bilal Ahmad Lone et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Lone, Bilal Ahmad
Karna, Shibendra Kumar Lal
Ahmad, Faiz
Shahi, Nerina
Pokharel, Yuba Raj
CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering
title CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering
title_full CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering
title_fullStr CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering
title_full_unstemmed CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering
title_short CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering
title_sort crispr/cas9 system: a bacterial tailor for genomic engineering
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167567/
https://www.ncbi.nlm.nih.gov/pubmed/30319822
http://dx.doi.org/10.1155/2018/3797214
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