Cargando…
CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering
Microbes use diverse defence strategies that allow them to withstand exposure to a variety of genome invaders such as bacteriophages and plasmids. One such defence strategy is the use of RNA guided endonuclease called CRISPR-associated (Cas) 9 protein. The Cas9 protein, derived from type II CRISPR/C...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167567/ https://www.ncbi.nlm.nih.gov/pubmed/30319822 http://dx.doi.org/10.1155/2018/3797214 |
_version_ | 1783360225580941312 |
---|---|
author | Lone, Bilal Ahmad Karna, Shibendra Kumar Lal Ahmad, Faiz Shahi, Nerina Pokharel, Yuba Raj |
author_facet | Lone, Bilal Ahmad Karna, Shibendra Kumar Lal Ahmad, Faiz Shahi, Nerina Pokharel, Yuba Raj |
author_sort | Lone, Bilal Ahmad |
collection | PubMed |
description | Microbes use diverse defence strategies that allow them to withstand exposure to a variety of genome invaders such as bacteriophages and plasmids. One such defence strategy is the use of RNA guided endonuclease called CRISPR-associated (Cas) 9 protein. The Cas9 protein, derived from type II CRISPR/Cas system, has been adapted as a versatile tool for genome targeting and engineering due to its simplicity and high efficiency over the earlier tools such as ZFNs and TALENs. With recent advancements, CRISPR/Cas9 technology has emerged as a revolutionary tool for modulating the genome in living cells and inspires innovative translational applications in different fields. In this paper we review the developments and its potential uses in the CRISPR/Cas9 technology as well as recent advancements in genome engineering using CRISPR/Cas9. |
format | Online Article Text |
id | pubmed-6167567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-61675672018-10-14 CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering Lone, Bilal Ahmad Karna, Shibendra Kumar Lal Ahmad, Faiz Shahi, Nerina Pokharel, Yuba Raj Genet Res Int Review Article Microbes use diverse defence strategies that allow them to withstand exposure to a variety of genome invaders such as bacteriophages and plasmids. One such defence strategy is the use of RNA guided endonuclease called CRISPR-associated (Cas) 9 protein. The Cas9 protein, derived from type II CRISPR/Cas system, has been adapted as a versatile tool for genome targeting and engineering due to its simplicity and high efficiency over the earlier tools such as ZFNs and TALENs. With recent advancements, CRISPR/Cas9 technology has emerged as a revolutionary tool for modulating the genome in living cells and inspires innovative translational applications in different fields. In this paper we review the developments and its potential uses in the CRISPR/Cas9 technology as well as recent advancements in genome engineering using CRISPR/Cas9. Hindawi 2018-09-18 /pmc/articles/PMC6167567/ /pubmed/30319822 http://dx.doi.org/10.1155/2018/3797214 Text en Copyright © 2018 Bilal Ahmad Lone et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Lone, Bilal Ahmad Karna, Shibendra Kumar Lal Ahmad, Faiz Shahi, Nerina Pokharel, Yuba Raj CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering |
title | CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering |
title_full | CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering |
title_fullStr | CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering |
title_full_unstemmed | CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering |
title_short | CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering |
title_sort | crispr/cas9 system: a bacterial tailor for genomic engineering |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167567/ https://www.ncbi.nlm.nih.gov/pubmed/30319822 http://dx.doi.org/10.1155/2018/3797214 |
work_keys_str_mv | AT lonebilalahmad crisprcas9systemabacterialtailorforgenomicengineering AT karnashibendrakumarlal crisprcas9systemabacterialtailorforgenomicengineering AT ahmadfaiz crisprcas9systemabacterialtailorforgenomicengineering AT shahinerina crisprcas9systemabacterialtailorforgenomicengineering AT pokharelyubaraj crisprcas9systemabacterialtailorforgenomicengineering |