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Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines

BACKGROUND: Clonal VDJ rearrangement of B/T cell receptors (B/TCRs) occurring during B/T lymphocyte development has been used as a marker to track the clonality of B/T cell populations. METHODS: We systematically profiled the B/T cell receptor repertoire of 936 cancer cell lines across a variety of...

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Autores principales: Tan, Kar-Tong, Ding, Ling-Wen, Sun, Qiao-Yang, Lao, Zhen-Tang, Chien, Wenwen, Ren, Xi, Xiao, Jin-Fen, Loh, Xin Yi, Xu, Liang, Lill, Michael, Mayakonda, Anand, Lin, De-Chen, Yang, Henry, Koeffler, H. Phillip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167786/
https://www.ncbi.nlm.nih.gov/pubmed/30285677
http://dx.doi.org/10.1186/s12885-018-4840-5
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author Tan, Kar-Tong
Ding, Ling-Wen
Sun, Qiao-Yang
Lao, Zhen-Tang
Chien, Wenwen
Ren, Xi
Xiao, Jin-Fen
Loh, Xin Yi
Xu, Liang
Lill, Michael
Mayakonda, Anand
Lin, De-Chen
Yang, Henry
Koeffler, H. Phillip
author_facet Tan, Kar-Tong
Ding, Ling-Wen
Sun, Qiao-Yang
Lao, Zhen-Tang
Chien, Wenwen
Ren, Xi
Xiao, Jin-Fen
Loh, Xin Yi
Xu, Liang
Lill, Michael
Mayakonda, Anand
Lin, De-Chen
Yang, Henry
Koeffler, H. Phillip
author_sort Tan, Kar-Tong
collection PubMed
description BACKGROUND: Clonal VDJ rearrangement of B/T cell receptors (B/TCRs) occurring during B/T lymphocyte development has been used as a marker to track the clonality of B/T cell populations. METHODS: We systematically profiled the B/T cell receptor repertoire of 936 cancer cell lines across a variety of cancer types as well as 462 Epstein-Barr Virus (EBV) transformed normal B lymphocyte lines using RNA sequencing data. RESULTS: Rearranged B/TCRs were readily detected in cell lines derived from lymphocytes, and subclonality or potential biclonality were found in a number of blood cancer cell lines. Clonal BCR/TCR rearrangements were detected in several blast phase CML lines and unexpectedly, one gastric cancer cell line (KE-97), reflecting a lymphoid origin of these cells. Notably, clonality was highly prevalent in EBV transformed B lymphocytes, suggesting either transformation only occurred in a few B cells or those with a growth advantage dominated the transformed population through clonal evolution. CONCLUSIONS: Our analysis reveals the complexity and heterogeneity of the BCR/TCR rearrangement repertoire and provides a unique insight into the clonality of lymphocyte derived cell lines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12885-018-4840-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-61677862018-10-09 Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines Tan, Kar-Tong Ding, Ling-Wen Sun, Qiao-Yang Lao, Zhen-Tang Chien, Wenwen Ren, Xi Xiao, Jin-Fen Loh, Xin Yi Xu, Liang Lill, Michael Mayakonda, Anand Lin, De-Chen Yang, Henry Koeffler, H. Phillip BMC Cancer Research Article BACKGROUND: Clonal VDJ rearrangement of B/T cell receptors (B/TCRs) occurring during B/T lymphocyte development has been used as a marker to track the clonality of B/T cell populations. METHODS: We systematically profiled the B/T cell receptor repertoire of 936 cancer cell lines across a variety of cancer types as well as 462 Epstein-Barr Virus (EBV) transformed normal B lymphocyte lines using RNA sequencing data. RESULTS: Rearranged B/TCRs were readily detected in cell lines derived from lymphocytes, and subclonality or potential biclonality were found in a number of blood cancer cell lines. Clonal BCR/TCR rearrangements were detected in several blast phase CML lines and unexpectedly, one gastric cancer cell line (KE-97), reflecting a lymphoid origin of these cells. Notably, clonality was highly prevalent in EBV transformed B lymphocytes, suggesting either transformation only occurred in a few B cells or those with a growth advantage dominated the transformed population through clonal evolution. CONCLUSIONS: Our analysis reveals the complexity and heterogeneity of the BCR/TCR rearrangement repertoire and provides a unique insight into the clonality of lymphocyte derived cell lines. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12885-018-4840-5) contains supplementary material, which is available to authorized users. BioMed Central 2018-10-01 /pmc/articles/PMC6167786/ /pubmed/30285677 http://dx.doi.org/10.1186/s12885-018-4840-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Tan, Kar-Tong
Ding, Ling-Wen
Sun, Qiao-Yang
Lao, Zhen-Tang
Chien, Wenwen
Ren, Xi
Xiao, Jin-Fen
Loh, Xin Yi
Xu, Liang
Lill, Michael
Mayakonda, Anand
Lin, De-Chen
Yang, Henry
Koeffler, H. Phillip
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines
title Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines
title_full Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines
title_fullStr Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines
title_full_unstemmed Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines
title_short Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines
title_sort profiling the b/t cell receptor repertoire of lymphocyte derived cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167786/
https://www.ncbi.nlm.nih.gov/pubmed/30285677
http://dx.doi.org/10.1186/s12885-018-4840-5
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