Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis

OBJECTIVE: We have previously codon-optimized a number of red fluorescent proteins for use in Mycobacterium tuberculosis (mCherry, tdTomato, Turbo-635). We aimed to expand this repertoire to include DsRed, another widely used and flexible red fluorescent protein. RESULTS: We generated expression con...

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Detalles Bibliográficos
Autores principales: Carroll, Paul, Muwanguzi-Karugaba, Julian, Parish, Tanya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167837/
https://www.ncbi.nlm.nih.gov/pubmed/30285840
http://dx.doi.org/10.1186/s13104-018-3798-3
Descripción
Sumario:OBJECTIVE: We have previously codon-optimized a number of red fluorescent proteins for use in Mycobacterium tuberculosis (mCherry, tdTomato, Turbo-635). We aimed to expand this repertoire to include DsRed, another widely used and flexible red fluorescent protein. RESULTS: We generated expression constructs with a full length DsRed under the control of one of three strong, constitutive promoters (P(hsp60), P(rpsA) or P(G13)) for use in mycobacteria. We confirmed that full length DsRed (225 amino acids) was expressed and fluoresced brightly. In contrast to mCherry, truncated versions of DsRed lacking several amino acids at the N-terminus were not functional. Thus, we have expanded the repertoire of optimized fluorescent proteins for mycobacteria.

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