Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis

OBJECTIVE: We have previously codon-optimized a number of red fluorescent proteins for use in Mycobacterium tuberculosis (mCherry, tdTomato, Turbo-635). We aimed to expand this repertoire to include DsRed, another widely used and flexible red fluorescent protein. RESULTS: We generated expression con...

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Detalles Bibliográficos
Autores principales: Carroll, Paul, Muwanguzi-Karugaba, Julian, Parish, Tanya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167837/
https://www.ncbi.nlm.nih.gov/pubmed/30285840
http://dx.doi.org/10.1186/s13104-018-3798-3
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author Carroll, Paul
Muwanguzi-Karugaba, Julian
Parish, Tanya
author_facet Carroll, Paul
Muwanguzi-Karugaba, Julian
Parish, Tanya
author_sort Carroll, Paul
collection PubMed
description OBJECTIVE: We have previously codon-optimized a number of red fluorescent proteins for use in Mycobacterium tuberculosis (mCherry, tdTomato, Turbo-635). We aimed to expand this repertoire to include DsRed, another widely used and flexible red fluorescent protein. RESULTS: We generated expression constructs with a full length DsRed under the control of one of three strong, constitutive promoters (P(hsp60), P(rpsA) or P(G13)) for use in mycobacteria. We confirmed that full length DsRed (225 amino acids) was expressed and fluoresced brightly. In contrast to mCherry, truncated versions of DsRed lacking several amino acids at the N-terminus were not functional. Thus, we have expanded the repertoire of optimized fluorescent proteins for mycobacteria.
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spelling pubmed-61678372018-10-09 Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis Carroll, Paul Muwanguzi-Karugaba, Julian Parish, Tanya BMC Res Notes Research Note OBJECTIVE: We have previously codon-optimized a number of red fluorescent proteins for use in Mycobacterium tuberculosis (mCherry, tdTomato, Turbo-635). We aimed to expand this repertoire to include DsRed, another widely used and flexible red fluorescent protein. RESULTS: We generated expression constructs with a full length DsRed under the control of one of three strong, constitutive promoters (P(hsp60), P(rpsA) or P(G13)) for use in mycobacteria. We confirmed that full length DsRed (225 amino acids) was expressed and fluoresced brightly. In contrast to mCherry, truncated versions of DsRed lacking several amino acids at the N-terminus were not functional. Thus, we have expanded the repertoire of optimized fluorescent proteins for mycobacteria. BioMed Central 2018-10-01 /pmc/articles/PMC6167837/ /pubmed/30285840 http://dx.doi.org/10.1186/s13104-018-3798-3 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Carroll, Paul
Muwanguzi-Karugaba, Julian
Parish, Tanya
Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis
title Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis
title_full Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis
title_fullStr Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis
title_full_unstemmed Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis
title_short Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis
title_sort codon-optimized dsred fluorescent protein for use in mycobacterium tuberculosis
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167837/
https://www.ncbi.nlm.nih.gov/pubmed/30285840
http://dx.doi.org/10.1186/s13104-018-3798-3
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AT parishtanya codonoptimizeddsredfluorescentproteinforuseinmycobacteriumtuberculosis

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