Bead-linked transposomes enable a normalization-free workflow for NGS library preparation

BACKGROUND: Transposome-based technologies have enabled the streamlined production of sequencer-ready DNA libraries; however, current methods are highly sensitive to the amount and quality of input nucleic acid. RESULTS: We describe a new library preparation technology (Nextera DNA Flex) that utiliz...

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Detalles Bibliográficos
Autores principales: Bruinsma, Stephen, Burgess, Joshua, Schlingman, Daniel, Czyz, Agata, Morrell, Natalie, Ballenger, Catherine, Meinholz, Heather, Brady, Lee, Khanna, Anupama, Freeberg, Lindsay, Jackson, Rosamond G, Mathonet, Pascale, Verity, Susan C, Slatter, Andrew F, Golshani, Rooz, Grunenwald, Haiying, Schroth, Gary P, Gormley, Niall A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167868/
https://www.ncbi.nlm.nih.gov/pubmed/30285621
http://dx.doi.org/10.1186/s12864-018-5096-9

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