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Experimental research into the potential therapeutic effect of GYY4137 on Ovariectomy-induced osteoporosis

BACKGROUND: Evidence has shown that endogenous H(2)S plays an important role in the physiological and pathophysiological processes of many organs. The study aimed to explore whether exogenous H(2)S has a potential therapeutic effect on a rat ovariectomy-induced model of osteoporosis. METHODS: The OV...

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Autores principales: Xu, Zhong-Shi, Dai, Feng, Chen, Ji, Lv, Meng, Cheng, Ji-Wu, Zhang, Xiao-Ming, Lin, Bo-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167887/
https://www.ncbi.nlm.nih.gov/pubmed/30305826
http://dx.doi.org/10.1186/s11658-018-0114-0
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author Xu, Zhong-Shi
Dai, Feng
Chen, Ji
Lv, Meng
Cheng, Ji-Wu
Zhang, Xiao-Ming
Lin, Bo-Wen
author_facet Xu, Zhong-Shi
Dai, Feng
Chen, Ji
Lv, Meng
Cheng, Ji-Wu
Zhang, Xiao-Ming
Lin, Bo-Wen
author_sort Xu, Zhong-Shi
collection PubMed
description BACKGROUND: Evidence has shown that endogenous H(2)S plays an important role in the physiological and pathophysiological processes of many organs. The study aimed to explore whether exogenous H(2)S has a potential therapeutic effect on a rat ovariectomy-induced model of osteoporosis. METHODS: The OVX osteoporosis model was established in female Sprague-Dawley rats by full bilateral ovariectomy. The rats were randomly divided into four groups, with the two experimental groups receiving an intraperitoneal injection of GYY4137 or sodium alendronate. The level of H(2)S in the plasma was determined and common laboratory indicators to diagnose osteoporosis, such as alkaline phosphatase (ALP) activity and the levels of osteocalcin (OCN), calcitonin, parathyroid hormone and leptin were measured. The bone mineral density (BMD) of the 4th and 5th lumbar vertebrae was measured using dual-energy X-ray absorptiometry. The maximum stress of femoral fracture was obtained through a three-point bending test of the femur. RESULTS: The OVX osteoporosis model was successfully established. GYY4137 was injected to increase the level of H(2)S in the plasma in one group, designated OVX-GYY during the observation period (p < 0.05). At 12 weeks, the BMD value of the fourth lumbar vertebra in the OVX-GYY group had increased (p < 0.05). The BMD femur value in the OVX-vehicle group had decreased (p < 0.05). Bilateral ovariectomy leads to biochemical disorders related to bone metabolism and hormone levels in rat plasma (all p < 0.05). Ovariectomy also reduced blood calcium, blood phosphate and calcitonin, and increased parathyroid hormone and leptin. The opposite results were obtained for the groups with alendronate sodium or GYY4137 treatment (all p < 0.05). CONCLUSIONS: Through the slow release of H(2)S, GYY4137 did an excellent job of simulating endogenous neuroendocrine gaseous signaling molecules. Exogenous H(2)S had a regulatory effect on osteoporosis in ovariectomized rats, showing potential value for the treatment of human postmenopausal osteoporosis.
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spelling pubmed-61678872018-10-10 Experimental research into the potential therapeutic effect of GYY4137 on Ovariectomy-induced osteoporosis Xu, Zhong-Shi Dai, Feng Chen, Ji Lv, Meng Cheng, Ji-Wu Zhang, Xiao-Ming Lin, Bo-Wen Cell Mol Biol Lett Research BACKGROUND: Evidence has shown that endogenous H(2)S plays an important role in the physiological and pathophysiological processes of many organs. The study aimed to explore whether exogenous H(2)S has a potential therapeutic effect on a rat ovariectomy-induced model of osteoporosis. METHODS: The OVX osteoporosis model was established in female Sprague-Dawley rats by full bilateral ovariectomy. The rats were randomly divided into four groups, with the two experimental groups receiving an intraperitoneal injection of GYY4137 or sodium alendronate. The level of H(2)S in the plasma was determined and common laboratory indicators to diagnose osteoporosis, such as alkaline phosphatase (ALP) activity and the levels of osteocalcin (OCN), calcitonin, parathyroid hormone and leptin were measured. The bone mineral density (BMD) of the 4th and 5th lumbar vertebrae was measured using dual-energy X-ray absorptiometry. The maximum stress of femoral fracture was obtained through a three-point bending test of the femur. RESULTS: The OVX osteoporosis model was successfully established. GYY4137 was injected to increase the level of H(2)S in the plasma in one group, designated OVX-GYY during the observation period (p < 0.05). At 12 weeks, the BMD value of the fourth lumbar vertebra in the OVX-GYY group had increased (p < 0.05). The BMD femur value in the OVX-vehicle group had decreased (p < 0.05). Bilateral ovariectomy leads to biochemical disorders related to bone metabolism and hormone levels in rat plasma (all p < 0.05). Ovariectomy also reduced blood calcium, blood phosphate and calcitonin, and increased parathyroid hormone and leptin. The opposite results were obtained for the groups with alendronate sodium or GYY4137 treatment (all p < 0.05). CONCLUSIONS: Through the slow release of H(2)S, GYY4137 did an excellent job of simulating endogenous neuroendocrine gaseous signaling molecules. Exogenous H(2)S had a regulatory effect on osteoporosis in ovariectomized rats, showing potential value for the treatment of human postmenopausal osteoporosis. BioMed Central 2018-10-01 /pmc/articles/PMC6167887/ /pubmed/30305826 http://dx.doi.org/10.1186/s11658-018-0114-0 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xu, Zhong-Shi
Dai, Feng
Chen, Ji
Lv, Meng
Cheng, Ji-Wu
Zhang, Xiao-Ming
Lin, Bo-Wen
Experimental research into the potential therapeutic effect of GYY4137 on Ovariectomy-induced osteoporosis
title Experimental research into the potential therapeutic effect of GYY4137 on Ovariectomy-induced osteoporosis
title_full Experimental research into the potential therapeutic effect of GYY4137 on Ovariectomy-induced osteoporosis
title_fullStr Experimental research into the potential therapeutic effect of GYY4137 on Ovariectomy-induced osteoporosis
title_full_unstemmed Experimental research into the potential therapeutic effect of GYY4137 on Ovariectomy-induced osteoporosis
title_short Experimental research into the potential therapeutic effect of GYY4137 on Ovariectomy-induced osteoporosis
title_sort experimental research into the potential therapeutic effect of gyy4137 on ovariectomy-induced osteoporosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167887/
https://www.ncbi.nlm.nih.gov/pubmed/30305826
http://dx.doi.org/10.1186/s11658-018-0114-0
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