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Non-destructive Plant Morphometric and Color Analyses Using an Optoelectronic 3D Color Microscope

Gene function discovery in plants, as other plant science quests, is aided by tools that image, document, and measure plant phenotypes. Tools that acquire images of plant organs and tissues at the microscopic level have evolved from qualitative documentation tools, to advanced tools where software-a...

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Autores principales: Lazcano-Ramírez, Hugo G., Gómez-Felipe, Andrea, Díaz-Ramírez, David, Durán-Medina, Yolanda, Sánchez-Segura, Lino, de Folter, Stefan, Marsch-Martínez, Nayelli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167917/
https://www.ncbi.nlm.nih.gov/pubmed/30319671
http://dx.doi.org/10.3389/fpls.2018.01409
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author Lazcano-Ramírez, Hugo G.
Gómez-Felipe, Andrea
Díaz-Ramírez, David
Durán-Medina, Yolanda
Sánchez-Segura, Lino
de Folter, Stefan
Marsch-Martínez, Nayelli
author_facet Lazcano-Ramírez, Hugo G.
Gómez-Felipe, Andrea
Díaz-Ramírez, David
Durán-Medina, Yolanda
Sánchez-Segura, Lino
de Folter, Stefan
Marsch-Martínez, Nayelli
author_sort Lazcano-Ramírez, Hugo G.
collection PubMed
description Gene function discovery in plants, as other plant science quests, is aided by tools that image, document, and measure plant phenotypes. Tools that acquire images of plant organs and tissues at the microscopic level have evolved from qualitative documentation tools, to advanced tools where software-assisted analysis of images extracts quantitative information that allows statistical analyses. They are useful to perform morphometric studies that describe plant physical characteristics and quantify phenotypes, aiding gene function discovery. In parallel, non-destructive, versatile, robust, and user friendly technologies have also been developed for surface topography analysis and quality control in the industrial manufacture sector, such as optoelectronic three-dimensional (3D) color microscopes. These microscopes combine optical lenses, electronic image sensors, motorized stages, graphics engines, and user friendly software to allow the visualization and inspection of objects of diverse sizes and shapes from different angles. This allow the integration of different automatically obtained images along the Z axis of an object, into a single image with a large depth-of-field, or a 3D model in color. In this work, we explored the performance of an optoelectronic microscope to study plant morphological phenotypes and plant surfaces in different model species. Furthermore, as a “proof-of-concept,” we included the phenotypic characterization (morphometric analyses at the organ level, color, and cell size measurements) of Arabidopsis mutant leaves. We found that the microscope tested is a suitable, practical, and fast tool to routinely and precisely analyze different plant organs and tissues, producing both high-quality, sharp color images and morphometric and color data in real time. It is fully compatible with live plant tissues (no sample preparation is required) and does not require special conditions, high maintenance, nor complex training. Therefore, though barely reported in plant scientific studies, optoelectronic microscopes should emerge as convenient and useful tools for phenotypic characterization in plant sciences.
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spelling pubmed-61679172018-10-12 Non-destructive Plant Morphometric and Color Analyses Using an Optoelectronic 3D Color Microscope Lazcano-Ramírez, Hugo G. Gómez-Felipe, Andrea Díaz-Ramírez, David Durán-Medina, Yolanda Sánchez-Segura, Lino de Folter, Stefan Marsch-Martínez, Nayelli Front Plant Sci Plant Science Gene function discovery in plants, as other plant science quests, is aided by tools that image, document, and measure plant phenotypes. Tools that acquire images of plant organs and tissues at the microscopic level have evolved from qualitative documentation tools, to advanced tools where software-assisted analysis of images extracts quantitative information that allows statistical analyses. They are useful to perform morphometric studies that describe plant physical characteristics and quantify phenotypes, aiding gene function discovery. In parallel, non-destructive, versatile, robust, and user friendly technologies have also been developed for surface topography analysis and quality control in the industrial manufacture sector, such as optoelectronic three-dimensional (3D) color microscopes. These microscopes combine optical lenses, electronic image sensors, motorized stages, graphics engines, and user friendly software to allow the visualization and inspection of objects of diverse sizes and shapes from different angles. This allow the integration of different automatically obtained images along the Z axis of an object, into a single image with a large depth-of-field, or a 3D model in color. In this work, we explored the performance of an optoelectronic microscope to study plant morphological phenotypes and plant surfaces in different model species. Furthermore, as a “proof-of-concept,” we included the phenotypic characterization (morphometric analyses at the organ level, color, and cell size measurements) of Arabidopsis mutant leaves. We found that the microscope tested is a suitable, practical, and fast tool to routinely and precisely analyze different plant organs and tissues, producing both high-quality, sharp color images and morphometric and color data in real time. It is fully compatible with live plant tissues (no sample preparation is required) and does not require special conditions, high maintenance, nor complex training. Therefore, though barely reported in plant scientific studies, optoelectronic microscopes should emerge as convenient and useful tools for phenotypic characterization in plant sciences. Frontiers Media S.A. 2018-09-25 /pmc/articles/PMC6167917/ /pubmed/30319671 http://dx.doi.org/10.3389/fpls.2018.01409 Text en Copyright © 2018 Lazcano-Ramírez, Gómez-Felipe, Díaz-Ramírez, Durán-Medina, Sánchez-Segura, de Folter and Marsch-Martínez. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lazcano-Ramírez, Hugo G.
Gómez-Felipe, Andrea
Díaz-Ramírez, David
Durán-Medina, Yolanda
Sánchez-Segura, Lino
de Folter, Stefan
Marsch-Martínez, Nayelli
Non-destructive Plant Morphometric and Color Analyses Using an Optoelectronic 3D Color Microscope
title Non-destructive Plant Morphometric and Color Analyses Using an Optoelectronic 3D Color Microscope
title_full Non-destructive Plant Morphometric and Color Analyses Using an Optoelectronic 3D Color Microscope
title_fullStr Non-destructive Plant Morphometric and Color Analyses Using an Optoelectronic 3D Color Microscope
title_full_unstemmed Non-destructive Plant Morphometric and Color Analyses Using an Optoelectronic 3D Color Microscope
title_short Non-destructive Plant Morphometric and Color Analyses Using an Optoelectronic 3D Color Microscope
title_sort non-destructive plant morphometric and color analyses using an optoelectronic 3d color microscope
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167917/
https://www.ncbi.nlm.nih.gov/pubmed/30319671
http://dx.doi.org/10.3389/fpls.2018.01409
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