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Genome Editing as a Tool for Fruit Ripening Manipulation

Over the last few years, a series of tools for genome editing have been developed, allowing the introduction of precise changes into plant genomes. These have included Zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, which is so far the most su...

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Autores principales: Martín-Pizarro, Carmen, Posé, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167941/
https://www.ncbi.nlm.nih.gov/pubmed/30319675
http://dx.doi.org/10.3389/fpls.2018.01415
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author Martín-Pizarro, Carmen
Posé, David
author_facet Martín-Pizarro, Carmen
Posé, David
author_sort Martín-Pizarro, Carmen
collection PubMed
description Over the last few years, a series of tools for genome editing have been developed, allowing the introduction of precise changes into plant genomes. These have included Zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, which is so far the most successful and commonly used approach for targeted and stable editing of DNA, due to its ease of use and low cost. CRISPR/Cas9 is now being widely used as a new plant breeding technique to improve commercially relevant crop species. Fruit ripening is a complex and genetically controlled developmental process that is essential for acquiring quality attributes of the fruit. Although the number of studies published to date using genome editing tools to molecularly understand or improve fruit ripening is scarce, in this review we discuss these achievements and how genome editing opens tremendous possibilities not only for functional studies of genes involved in fruit ripening, but also to generate non-transgenic plants with an improved fruit quality.
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spelling pubmed-61679412018-10-12 Genome Editing as a Tool for Fruit Ripening Manipulation Martín-Pizarro, Carmen Posé, David Front Plant Sci Plant Science Over the last few years, a series of tools for genome editing have been developed, allowing the introduction of precise changes into plant genomes. These have included Zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, which is so far the most successful and commonly used approach for targeted and stable editing of DNA, due to its ease of use and low cost. CRISPR/Cas9 is now being widely used as a new plant breeding technique to improve commercially relevant crop species. Fruit ripening is a complex and genetically controlled developmental process that is essential for acquiring quality attributes of the fruit. Although the number of studies published to date using genome editing tools to molecularly understand or improve fruit ripening is scarce, in this review we discuss these achievements and how genome editing opens tremendous possibilities not only for functional studies of genes involved in fruit ripening, but also to generate non-transgenic plants with an improved fruit quality. Frontiers Media S.A. 2018-09-25 /pmc/articles/PMC6167941/ /pubmed/30319675 http://dx.doi.org/10.3389/fpls.2018.01415 Text en Copyright © 2018 Martín-Pizarro and Posé. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Martín-Pizarro, Carmen
Posé, David
Genome Editing as a Tool for Fruit Ripening Manipulation
title Genome Editing as a Tool for Fruit Ripening Manipulation
title_full Genome Editing as a Tool for Fruit Ripening Manipulation
title_fullStr Genome Editing as a Tool for Fruit Ripening Manipulation
title_full_unstemmed Genome Editing as a Tool for Fruit Ripening Manipulation
title_short Genome Editing as a Tool for Fruit Ripening Manipulation
title_sort genome editing as a tool for fruit ripening manipulation
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167941/
https://www.ncbi.nlm.nih.gov/pubmed/30319675
http://dx.doi.org/10.3389/fpls.2018.01415
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