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Fluorescence properties of curcumin-loaded nanoparticles for cell tracking
BACKGROUND: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. MATERIALS AND METHODS...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168007/ https://www.ncbi.nlm.nih.gov/pubmed/30319253 http://dx.doi.org/10.2147/IJN.S171099 |
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author | Mogharbel, Bassam Felipe Francisco, Julio Cesar Irioda, Ana Carolina Dziedzic, Dilcele Silva Moreira Ferreira, Priscila Elias de Souza, Daiany de Souza, Carolina Maria Costa Oliveira Neto, Nelson Bergonse Guarita-Souza, Luiz Cesar Franco, Celia Regina Cavichiolo Nakamura, Celso Vataru Kaplum, Vanessa Mazzarino, Letícia Lemos-Senna, Elenara Borsali, Redouane Soto, Paula A Setton-Avruj, Patricia Abdelwahid, Eltyeb de Carvalho, Katherine Athayde Teixeira |
author_facet | Mogharbel, Bassam Felipe Francisco, Julio Cesar Irioda, Ana Carolina Dziedzic, Dilcele Silva Moreira Ferreira, Priscila Elias de Souza, Daiany de Souza, Carolina Maria Costa Oliveira Neto, Nelson Bergonse Guarita-Souza, Luiz Cesar Franco, Celia Regina Cavichiolo Nakamura, Celso Vataru Kaplum, Vanessa Mazzarino, Letícia Lemos-Senna, Elenara Borsali, Redouane Soto, Paula A Setton-Avruj, Patricia Abdelwahid, Eltyeb de Carvalho, Katherine Athayde Teixeira |
author_sort | Mogharbel, Bassam Felipe |
collection | PubMed |
description | BACKGROUND: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. MATERIALS AND METHODS: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. RESULTS: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. CONCLUSION: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies. |
format | Online Article Text |
id | pubmed-6168007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-61680072018-10-12 Fluorescence properties of curcumin-loaded nanoparticles for cell tracking Mogharbel, Bassam Felipe Francisco, Julio Cesar Irioda, Ana Carolina Dziedzic, Dilcele Silva Moreira Ferreira, Priscila Elias de Souza, Daiany de Souza, Carolina Maria Costa Oliveira Neto, Nelson Bergonse Guarita-Souza, Luiz Cesar Franco, Celia Regina Cavichiolo Nakamura, Celso Vataru Kaplum, Vanessa Mazzarino, Letícia Lemos-Senna, Elenara Borsali, Redouane Soto, Paula A Setton-Avruj, Patricia Abdelwahid, Eltyeb de Carvalho, Katherine Athayde Teixeira Int J Nanomedicine Original Research BACKGROUND: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. MATERIALS AND METHODS: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. RESULTS: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. CONCLUSION: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies. Dove Medical Press 2018-09-28 /pmc/articles/PMC6168007/ /pubmed/30319253 http://dx.doi.org/10.2147/IJN.S171099 Text en © 2018 Mogharbel et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Mogharbel, Bassam Felipe Francisco, Julio Cesar Irioda, Ana Carolina Dziedzic, Dilcele Silva Moreira Ferreira, Priscila Elias de Souza, Daiany de Souza, Carolina Maria Costa Oliveira Neto, Nelson Bergonse Guarita-Souza, Luiz Cesar Franco, Celia Regina Cavichiolo Nakamura, Celso Vataru Kaplum, Vanessa Mazzarino, Letícia Lemos-Senna, Elenara Borsali, Redouane Soto, Paula A Setton-Avruj, Patricia Abdelwahid, Eltyeb de Carvalho, Katherine Athayde Teixeira Fluorescence properties of curcumin-loaded nanoparticles for cell tracking |
title | Fluorescence properties of curcumin-loaded nanoparticles for cell tracking |
title_full | Fluorescence properties of curcumin-loaded nanoparticles for cell tracking |
title_fullStr | Fluorescence properties of curcumin-loaded nanoparticles for cell tracking |
title_full_unstemmed | Fluorescence properties of curcumin-loaded nanoparticles for cell tracking |
title_short | Fluorescence properties of curcumin-loaded nanoparticles for cell tracking |
title_sort | fluorescence properties of curcumin-loaded nanoparticles for cell tracking |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168007/ https://www.ncbi.nlm.nih.gov/pubmed/30319253 http://dx.doi.org/10.2147/IJN.S171099 |
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