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Fluorescence properties of curcumin-loaded nanoparticles for cell tracking

BACKGROUND: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. MATERIALS AND METHODS...

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Autores principales: Mogharbel, Bassam Felipe, Francisco, Julio Cesar, Irioda, Ana Carolina, Dziedzic, Dilcele Silva Moreira, Ferreira, Priscila Elias, de Souza, Daiany, de Souza, Carolina Maria Costa Oliveira, Neto, Nelson Bergonse, Guarita-Souza, Luiz Cesar, Franco, Celia Regina Cavichiolo, Nakamura, Celso Vataru, Kaplum, Vanessa, Mazzarino, Letícia, Lemos-Senna, Elenara, Borsali, Redouane, Soto, Paula A, Setton-Avruj, Patricia, Abdelwahid, Eltyeb, de Carvalho, Katherine Athayde Teixeira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168007/
https://www.ncbi.nlm.nih.gov/pubmed/30319253
http://dx.doi.org/10.2147/IJN.S171099
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author Mogharbel, Bassam Felipe
Francisco, Julio Cesar
Irioda, Ana Carolina
Dziedzic, Dilcele Silva Moreira
Ferreira, Priscila Elias
de Souza, Daiany
de Souza, Carolina Maria Costa Oliveira
Neto, Nelson Bergonse
Guarita-Souza, Luiz Cesar
Franco, Celia Regina Cavichiolo
Nakamura, Celso Vataru
Kaplum, Vanessa
Mazzarino, Letícia
Lemos-Senna, Elenara
Borsali, Redouane
Soto, Paula A
Setton-Avruj, Patricia
Abdelwahid, Eltyeb
de Carvalho, Katherine Athayde Teixeira
author_facet Mogharbel, Bassam Felipe
Francisco, Julio Cesar
Irioda, Ana Carolina
Dziedzic, Dilcele Silva Moreira
Ferreira, Priscila Elias
de Souza, Daiany
de Souza, Carolina Maria Costa Oliveira
Neto, Nelson Bergonse
Guarita-Souza, Luiz Cesar
Franco, Celia Regina Cavichiolo
Nakamura, Celso Vataru
Kaplum, Vanessa
Mazzarino, Letícia
Lemos-Senna, Elenara
Borsali, Redouane
Soto, Paula A
Setton-Avruj, Patricia
Abdelwahid, Eltyeb
de Carvalho, Katherine Athayde Teixeira
author_sort Mogharbel, Bassam Felipe
collection PubMed
description BACKGROUND: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. MATERIALS AND METHODS: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. RESULTS: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. CONCLUSION: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.
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spelling pubmed-61680072018-10-12 Fluorescence properties of curcumin-loaded nanoparticles for cell tracking Mogharbel, Bassam Felipe Francisco, Julio Cesar Irioda, Ana Carolina Dziedzic, Dilcele Silva Moreira Ferreira, Priscila Elias de Souza, Daiany de Souza, Carolina Maria Costa Oliveira Neto, Nelson Bergonse Guarita-Souza, Luiz Cesar Franco, Celia Regina Cavichiolo Nakamura, Celso Vataru Kaplum, Vanessa Mazzarino, Letícia Lemos-Senna, Elenara Borsali, Redouane Soto, Paula A Setton-Avruj, Patricia Abdelwahid, Eltyeb de Carvalho, Katherine Athayde Teixeira Int J Nanomedicine Original Research BACKGROUND: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. MATERIALS AND METHODS: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. RESULTS: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. CONCLUSION: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies. Dove Medical Press 2018-09-28 /pmc/articles/PMC6168007/ /pubmed/30319253 http://dx.doi.org/10.2147/IJN.S171099 Text en © 2018 Mogharbel et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Mogharbel, Bassam Felipe
Francisco, Julio Cesar
Irioda, Ana Carolina
Dziedzic, Dilcele Silva Moreira
Ferreira, Priscila Elias
de Souza, Daiany
de Souza, Carolina Maria Costa Oliveira
Neto, Nelson Bergonse
Guarita-Souza, Luiz Cesar
Franco, Celia Regina Cavichiolo
Nakamura, Celso Vataru
Kaplum, Vanessa
Mazzarino, Letícia
Lemos-Senna, Elenara
Borsali, Redouane
Soto, Paula A
Setton-Avruj, Patricia
Abdelwahid, Eltyeb
de Carvalho, Katherine Athayde Teixeira
Fluorescence properties of curcumin-loaded nanoparticles for cell tracking
title Fluorescence properties of curcumin-loaded nanoparticles for cell tracking
title_full Fluorescence properties of curcumin-loaded nanoparticles for cell tracking
title_fullStr Fluorescence properties of curcumin-loaded nanoparticles for cell tracking
title_full_unstemmed Fluorescence properties of curcumin-loaded nanoparticles for cell tracking
title_short Fluorescence properties of curcumin-loaded nanoparticles for cell tracking
title_sort fluorescence properties of curcumin-loaded nanoparticles for cell tracking
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168007/
https://www.ncbi.nlm.nih.gov/pubmed/30319253
http://dx.doi.org/10.2147/IJN.S171099
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