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Actin and microtubule cross talk mediates persistent polarized growth

Coordination between actin and microtubules is important for numerous cellular processes in diverse eukaryotes. In plants, tip-growing cells require actin for cell expansion and microtubules for orientation of cell expansion, but how the two cytoskeletons are linked is an open question. In tip-growi...

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Detalles Bibliográficos
Autores principales: Wu, Shu-Zon, Bezanilla, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168251/
https://www.ncbi.nlm.nih.gov/pubmed/30061106
http://dx.doi.org/10.1083/jcb.201802039
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author Wu, Shu-Zon
Bezanilla, Magdalena
author_facet Wu, Shu-Zon
Bezanilla, Magdalena
author_sort Wu, Shu-Zon
collection PubMed
description Coordination between actin and microtubules is important for numerous cellular processes in diverse eukaryotes. In plants, tip-growing cells require actin for cell expansion and microtubules for orientation of cell expansion, but how the two cytoskeletons are linked is an open question. In tip-growing cells of the moss Physcomitrella patens, we show that an actin cluster near the cell apex dictates the direction of rapid cell expansion. Formation of this structure depends on the convergence of microtubules near the cell tip. We discovered that microtubule convergence requires class VIII myosin function, and actin is necessary for myosin VIII–mediated focusing of microtubules. The loss of myosin VIII function affects both networks, indicating functional connections among the three cytoskeletal components. Our data suggest that microtubules direct localization of formins, actin nucleation factors, that generate actin filaments further focusing microtubules, thereby establishing a positive feedback loop ensuring that actin polymerization and cell expansion occur at a defined site resulting in persistent polarized growth.
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spelling pubmed-61682512019-04-01 Actin and microtubule cross talk mediates persistent polarized growth Wu, Shu-Zon Bezanilla, Magdalena J Cell Biol Research Articles Coordination between actin and microtubules is important for numerous cellular processes in diverse eukaryotes. In plants, tip-growing cells require actin for cell expansion and microtubules for orientation of cell expansion, but how the two cytoskeletons are linked is an open question. In tip-growing cells of the moss Physcomitrella patens, we show that an actin cluster near the cell apex dictates the direction of rapid cell expansion. Formation of this structure depends on the convergence of microtubules near the cell tip. We discovered that microtubule convergence requires class VIII myosin function, and actin is necessary for myosin VIII–mediated focusing of microtubules. The loss of myosin VIII function affects both networks, indicating functional connections among the three cytoskeletal components. Our data suggest that microtubules direct localization of formins, actin nucleation factors, that generate actin filaments further focusing microtubules, thereby establishing a positive feedback loop ensuring that actin polymerization and cell expansion occur at a defined site resulting in persistent polarized growth. Rockefeller University Press 2018-10-01 /pmc/articles/PMC6168251/ /pubmed/30061106 http://dx.doi.org/10.1083/jcb.201802039 Text en © 2018 Wu and Bezanilla http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Wu, Shu-Zon
Bezanilla, Magdalena
Actin and microtubule cross talk mediates persistent polarized growth
title Actin and microtubule cross talk mediates persistent polarized growth
title_full Actin and microtubule cross talk mediates persistent polarized growth
title_fullStr Actin and microtubule cross talk mediates persistent polarized growth
title_full_unstemmed Actin and microtubule cross talk mediates persistent polarized growth
title_short Actin and microtubule cross talk mediates persistent polarized growth
title_sort actin and microtubule cross talk mediates persistent polarized growth
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168251/
https://www.ncbi.nlm.nih.gov/pubmed/30061106
http://dx.doi.org/10.1083/jcb.201802039
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