Reducing effects of particle adsorption to the air-water interface in cryoEM

Most protein particles prepared in vitreous ice for single particle cryo-electron microscopy are adsorbed to air-water or substrate-water interfaces, potentially causing particles to adopt preferred orientations. Using the Spotiton robot and nanowire grids, we can reduce some of the deleterious effe...

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Autores principales: Noble, Alex J., Wei, Hui, Dandey, Venkata P., Zhang, Zhening, Zi Tan, Yong, Potter, Clinton S., Carragher, Bridget
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168394/
https://www.ncbi.nlm.nih.gov/pubmed/30250056
http://dx.doi.org/10.1038/s41592-018-0139-3
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author Noble, Alex J.
Wei, Hui
Dandey, Venkata P.
Zhang, Zhening
Zi Tan, Yong
Potter, Clinton S.
Carragher, Bridget
author_facet Noble, Alex J.
Wei, Hui
Dandey, Venkata P.
Zhang, Zhening
Zi Tan, Yong
Potter, Clinton S.
Carragher, Bridget
author_sort Noble, Alex J.
collection PubMed
description Most protein particles prepared in vitreous ice for single particle cryo-electron microscopy are adsorbed to air-water or substrate-water interfaces, potentially causing particles to adopt preferred orientations. Using the Spotiton robot and nanowire grids, we can reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrate this by using single particle cryoEM and cryoET on three biological samples.
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spelling pubmed-61683942019-03-24 Reducing effects of particle adsorption to the air-water interface in cryoEM Noble, Alex J. Wei, Hui Dandey, Venkata P. Zhang, Zhening Zi Tan, Yong Potter, Clinton S. Carragher, Bridget Nat Methods Article Most protein particles prepared in vitreous ice for single particle cryo-electron microscopy are adsorbed to air-water or substrate-water interfaces, potentially causing particles to adopt preferred orientations. Using the Spotiton robot and nanowire grids, we can reduce some of the deleterious effects of the air-water interface by decreasing the dwell time of particles in thin liquid films. We demonstrate this by using single particle cryoEM and cryoET on three biological samples. 2018-09-24 2018-10 /pmc/articles/PMC6168394/ /pubmed/30250056 http://dx.doi.org/10.1038/s41592-018-0139-3 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Noble, Alex J.
Wei, Hui
Dandey, Venkata P.
Zhang, Zhening
Zi Tan, Yong
Potter, Clinton S.
Carragher, Bridget
Reducing effects of particle adsorption to the air-water interface in cryoEM
title Reducing effects of particle adsorption to the air-water interface in cryoEM
title_full Reducing effects of particle adsorption to the air-water interface in cryoEM
title_fullStr Reducing effects of particle adsorption to the air-water interface in cryoEM
title_full_unstemmed Reducing effects of particle adsorption to the air-water interface in cryoEM
title_short Reducing effects of particle adsorption to the air-water interface in cryoEM
title_sort reducing effects of particle adsorption to the air-water interface in cryoem
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168394/
https://www.ncbi.nlm.nih.gov/pubmed/30250056
http://dx.doi.org/10.1038/s41592-018-0139-3
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