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Control of proline utilization by the Lrp-like regulator PutR in Caulobacter crescentus

Cellular metabolism recently emerged as a central player modulating the bacterial cell cycle. The Alphaproteobacterium Caulobacter crescentus appears as one of the best models to study these connections, but its metabolism is still poorly characterized. Considering that it lives in oligotrophic envi...

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Autores principales: Mouammine, Annabelle, Eich, Katharina, Frandi, Antonio, Collier, Justine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168545/
https://www.ncbi.nlm.nih.gov/pubmed/30279528
http://dx.doi.org/10.1038/s41598-018-32660-3
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author Mouammine, Annabelle
Eich, Katharina
Frandi, Antonio
Collier, Justine
author_facet Mouammine, Annabelle
Eich, Katharina
Frandi, Antonio
Collier, Justine
author_sort Mouammine, Annabelle
collection PubMed
description Cellular metabolism recently emerged as a central player modulating the bacterial cell cycle. The Alphaproteobacterium Caulobacter crescentus appears as one of the best models to study these connections, but its metabolism is still poorly characterized. Considering that it lives in oligotrophic environments, its capacity to use amino-acids is often critical for its growth. Here, we characterized the C. crescentus PutA bi-functional enzyme and showed that it is required for the utilization of proline as a carbon source. We also found that putA transcription and proline utilization by PutA are strictly dependent on the Lrp-like PutR activator. The activation of putA by PutR needs proline, which most likely acts as an effector molecule for PutR. Surprisingly, we also observed that an over-production of PutR leads to cell elongation in liquid medium containing proline, while it inhibits colony formation even in the absence of proline on solid medium. These cell division and growth defects were equally pronounced in a ΔputA mutant background, indicating that PutR can play other roles beyond the control of proline catabolism. Altogether, these findings suggest that PutR might connect central metabolism with cell cycle processes.
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spelling pubmed-61685452018-10-05 Control of proline utilization by the Lrp-like regulator PutR in Caulobacter crescentus Mouammine, Annabelle Eich, Katharina Frandi, Antonio Collier, Justine Sci Rep Article Cellular metabolism recently emerged as a central player modulating the bacterial cell cycle. The Alphaproteobacterium Caulobacter crescentus appears as one of the best models to study these connections, but its metabolism is still poorly characterized. Considering that it lives in oligotrophic environments, its capacity to use amino-acids is often critical for its growth. Here, we characterized the C. crescentus PutA bi-functional enzyme and showed that it is required for the utilization of proline as a carbon source. We also found that putA transcription and proline utilization by PutA are strictly dependent on the Lrp-like PutR activator. The activation of putA by PutR needs proline, which most likely acts as an effector molecule for PutR. Surprisingly, we also observed that an over-production of PutR leads to cell elongation in liquid medium containing proline, while it inhibits colony formation even in the absence of proline on solid medium. These cell division and growth defects were equally pronounced in a ΔputA mutant background, indicating that PutR can play other roles beyond the control of proline catabolism. Altogether, these findings suggest that PutR might connect central metabolism with cell cycle processes. Nature Publishing Group UK 2018-10-02 /pmc/articles/PMC6168545/ /pubmed/30279528 http://dx.doi.org/10.1038/s41598-018-32660-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mouammine, Annabelle
Eich, Katharina
Frandi, Antonio
Collier, Justine
Control of proline utilization by the Lrp-like regulator PutR in Caulobacter crescentus
title Control of proline utilization by the Lrp-like regulator PutR in Caulobacter crescentus
title_full Control of proline utilization by the Lrp-like regulator PutR in Caulobacter crescentus
title_fullStr Control of proline utilization by the Lrp-like regulator PutR in Caulobacter crescentus
title_full_unstemmed Control of proline utilization by the Lrp-like regulator PutR in Caulobacter crescentus
title_short Control of proline utilization by the Lrp-like regulator PutR in Caulobacter crescentus
title_sort control of proline utilization by the lrp-like regulator putr in caulobacter crescentus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168545/
https://www.ncbi.nlm.nih.gov/pubmed/30279528
http://dx.doi.org/10.1038/s41598-018-32660-3
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