Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV–ve Oral Cancer Stem Cells

Aim: To investigate the role of a herbal antioxidative compound curcumin on cell proliferation, orosphere formation and miRNA-21 expression in HPV16+ve/–ve oral cancer stem cells. Materials and Methods: Oral cancer stem cells were isolated from HPV+ve/HPV–ve oral cancer cell lines by FACS and stemne...

Descripción completa

Detalles Bibliográficos
Autores principales: Bano, Nasreen, Yadav, Manisha, Das, Bhudev C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Oncology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168628/
https://www.ncbi.nlm.nih.gov/pubmed/30319975
http://dx.doi.org/10.3389/fonc.2018.00412
_version_ 1783360390821838848
author Bano, Nasreen
Yadav, Manisha
Das, Bhudev C.
author_facet Bano, Nasreen
Yadav, Manisha
Das, Bhudev C.
author_sort Bano, Nasreen
collection PubMed
description Aim: To investigate the role of a herbal antioxidative compound curcumin on cell proliferation, orosphere formation and miRNA-21 expression in HPV16+ve/–ve oral cancer stem cells. Materials and Methods: Oral cancer stem cells were isolated from HPV+ve/HPV–ve oral cancer cell lines by FACS and stemness markers. MTT, spheroid assay and qRT-PCR were employed to examine the effects of curcumin. Results: Curcumin treatment in micromolar concentration (0–50 μM) demonstrated significant differential inhibition in CSC proliferation, orosphere formation and miRNA-21 expression in a dose dependent manner, the effect being highly pronounced in HPV positive CSCs. Conclusion: The strong and dose-dependent inhibitory effects of curcumin on cell proliferation, stemness and miRNA appear to be due to its chemosensitizing and anticancer effects on OSCC-CSCs.
format Online
Article
Text
id pubmed-6168628
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-61686282018-10-12 Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV–ve Oral Cancer Stem Cells Bano, Nasreen Yadav, Manisha Das, Bhudev C. Front Oncol Oncology Aim: To investigate the role of a herbal antioxidative compound curcumin on cell proliferation, orosphere formation and miRNA-21 expression in HPV16+ve/–ve oral cancer stem cells. Materials and Methods: Oral cancer stem cells were isolated from HPV+ve/HPV–ve oral cancer cell lines by FACS and stemness markers. MTT, spheroid assay and qRT-PCR were employed to examine the effects of curcumin. Results: Curcumin treatment in micromolar concentration (0–50 μM) demonstrated significant differential inhibition in CSC proliferation, orosphere formation and miRNA-21 expression in a dose dependent manner, the effect being highly pronounced in HPV positive CSCs. Conclusion: The strong and dose-dependent inhibitory effects of curcumin on cell proliferation, stemness and miRNA appear to be due to its chemosensitizing and anticancer effects on OSCC-CSCs. Frontiers Media S.A. 2018-09-26 /pmc/articles/PMC6168628/ /pubmed/30319975 http://dx.doi.org/10.3389/fonc.2018.00412 Text en Copyright © 2018 Bano, Yadav and Das. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Bano, Nasreen
Yadav, Manisha
Das, Bhudev C.
Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV–ve Oral Cancer Stem Cells
title Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV–ve Oral Cancer Stem Cells
title_full Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV–ve Oral Cancer Stem Cells
title_fullStr Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV–ve Oral Cancer Stem Cells
title_full_unstemmed Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV–ve Oral Cancer Stem Cells
title_short Differential Inhibitory Effects of Curcumin Between HPV+ve and HPV–ve Oral Cancer Stem Cells
title_sort differential inhibitory effects of curcumin between hpv+ve and hpv–ve oral cancer stem cells
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168628/
https://www.ncbi.nlm.nih.gov/pubmed/30319975
http://dx.doi.org/10.3389/fonc.2018.00412
work_keys_str_mv AT banonasreen differentialinhibitoryeffectsofcurcuminbetweenhpvveandhpvveoralcancerstemcells
AT yadavmanisha differentialinhibitoryeffectsofcurcuminbetweenhpvveandhpvveoralcancerstemcells
AT dasbhudevc differentialinhibitoryeffectsofcurcuminbetweenhpvveandhpvveoralcancerstemcells

Ejemplares similares