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Circular RNA expression profile of spleen in a Clostridium perfringens type C‐induced piglet model of necrotizing enteritis

Clostridium perfringens type C is a pathogen that causes necrotizing enteritis (NE), which is an intestinal tract disease in piglets. The pathogenesis of C. perfringens type C‐induced NE is still unclear, leading to a lack of effective therapies. Earlier studies have reported that circular RNAs (cir...

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Autores principales: Yan, Zunqiang, Jiang, Tiantuan, Wang, Pengfei, Huang, Xiaoyu, Yang, Qiaoli, Sun, Wenyang, Gun, Shuangbao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168697/
https://www.ncbi.nlm.nih.gov/pubmed/30338222
http://dx.doi.org/10.1002/2211-5463.12512
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author Yan, Zunqiang
Jiang, Tiantuan
Wang, Pengfei
Huang, Xiaoyu
Yang, Qiaoli
Sun, Wenyang
Gun, Shuangbao
author_facet Yan, Zunqiang
Jiang, Tiantuan
Wang, Pengfei
Huang, Xiaoyu
Yang, Qiaoli
Sun, Wenyang
Gun, Shuangbao
author_sort Yan, Zunqiang
collection PubMed
description Clostridium perfringens type C is a pathogen that causes necrotizing enteritis (NE), which is an intestinal tract disease in piglets. The pathogenesis of C. perfringens type C‐induced NE is still unclear, leading to a lack of effective therapies. Earlier studies have reported that circular RNAs (circRNAs) are involved in the pathogenic processes of various diseases. However, it is not known if circRNAs in spleen play a role in C. perfringens type C infection in NE. To address this question, we infected 7‐day‐old piglets with C. perfringens type C to induce NE. Hematoxylin and eosin staining of small intestine revealed inflammation, atrophy and shedding of intestinal villi, and intestinal mucosal necrosis. We observed increased expression of cytokine genes (such as IL‐1β and IL‐6) and inflammation in the spleen. In addition, we used RNA‐seq and bioinformatics analysis to examine changes in circRNA expression. A total of 103 circRNAs were found to be differentially expressed in NE, and Gene Ontology analysis revealed that the genes producing differentially expressed circRNAs were enriched in regulation of the cellular metabolic process protein binding. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the genes producing differentially expressed circRNAs were involved in the tumor necrosis factor signaling pathway, T cell receptor signaling pathway and nuclear factor‐κB signaling pathway. Finally, we found eight circRNAs (including circ_0002220 and circ_0000821) that are related to NE. Therefore, our study provides new insights into the mechanisms underlying C. perfringens type C infection in piglets.
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spelling pubmed-61686972018-10-18 Circular RNA expression profile of spleen in a Clostridium perfringens type C‐induced piglet model of necrotizing enteritis Yan, Zunqiang Jiang, Tiantuan Wang, Pengfei Huang, Xiaoyu Yang, Qiaoli Sun, Wenyang Gun, Shuangbao FEBS Open Bio Research Articles Clostridium perfringens type C is a pathogen that causes necrotizing enteritis (NE), which is an intestinal tract disease in piglets. The pathogenesis of C. perfringens type C‐induced NE is still unclear, leading to a lack of effective therapies. Earlier studies have reported that circular RNAs (circRNAs) are involved in the pathogenic processes of various diseases. However, it is not known if circRNAs in spleen play a role in C. perfringens type C infection in NE. To address this question, we infected 7‐day‐old piglets with C. perfringens type C to induce NE. Hematoxylin and eosin staining of small intestine revealed inflammation, atrophy and shedding of intestinal villi, and intestinal mucosal necrosis. We observed increased expression of cytokine genes (such as IL‐1β and IL‐6) and inflammation in the spleen. In addition, we used RNA‐seq and bioinformatics analysis to examine changes in circRNA expression. A total of 103 circRNAs were found to be differentially expressed in NE, and Gene Ontology analysis revealed that the genes producing differentially expressed circRNAs were enriched in regulation of the cellular metabolic process protein binding. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the genes producing differentially expressed circRNAs were involved in the tumor necrosis factor signaling pathway, T cell receptor signaling pathway and nuclear factor‐κB signaling pathway. Finally, we found eight circRNAs (including circ_0002220 and circ_0000821) that are related to NE. Therefore, our study provides new insights into the mechanisms underlying C. perfringens type C infection in piglets. John Wiley and Sons Inc. 2018-09-17 /pmc/articles/PMC6168697/ /pubmed/30338222 http://dx.doi.org/10.1002/2211-5463.12512 Text en © 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Yan, Zunqiang
Jiang, Tiantuan
Wang, Pengfei
Huang, Xiaoyu
Yang, Qiaoli
Sun, Wenyang
Gun, Shuangbao
Circular RNA expression profile of spleen in a Clostridium perfringens type C‐induced piglet model of necrotizing enteritis
title Circular RNA expression profile of spleen in a Clostridium perfringens type C‐induced piglet model of necrotizing enteritis
title_full Circular RNA expression profile of spleen in a Clostridium perfringens type C‐induced piglet model of necrotizing enteritis
title_fullStr Circular RNA expression profile of spleen in a Clostridium perfringens type C‐induced piglet model of necrotizing enteritis
title_full_unstemmed Circular RNA expression profile of spleen in a Clostridium perfringens type C‐induced piglet model of necrotizing enteritis
title_short Circular RNA expression profile of spleen in a Clostridium perfringens type C‐induced piglet model of necrotizing enteritis
title_sort circular rna expression profile of spleen in a clostridium perfringens type c‐induced piglet model of necrotizing enteritis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168697/
https://www.ncbi.nlm.nih.gov/pubmed/30338222
http://dx.doi.org/10.1002/2211-5463.12512
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