In vitro α-amylase, α-glucosidase, lipase inhibitory and cytotoxic activities of tuber extracts of Kedrostis africana (L.) Cogn

Kedrostis africana, is a tuberous plant commonly used by traditional healers in the Eastern Cape Province of South Africa for the management of obesity. The aim of this study was to investigate the antiobesity and cytotoxic effects of Kedrostis africana extracts in vitro The α-amylase, α-glucosidase and lipase inhibitory activities of aqueous and ethanol extracts of Kedrostis africana tuber were investigated while the cytotoxic effects of these extracts were analyzed using Hoechst 33342 and propidium iodide (PI) dual staining in combination with Molecular Devices ImageXpress Micro XLS Widefield microscope for high content analysis on human cervical (HeLa) cell line. The ethanol extract exhibited the strongest inhibitory effect on pancreatic lipase (IC(50) = 381.86 μg/ml) and on α-glucosidase (IC(50) = 157.99 μg/mL) while the aqueous extract has strongest α-amylase (IC(50) = 439.45 μg/ml). Both tuber extracts were found nontoxic at tested concentrations on HeLa cell lines as confirmed by the Hoechst 33342 and propidium iodide dual staining respectively. This study revealed that both the aqueous and ethanol tuber extract of K. africana exerts a certain degree of inhibitory effect on α-amylase, α-glucosidase and lipase and were also nontoxic to HeLa cell line at tested concentrations.