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In vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E™
A colloid with a species specific silane-coated, silica-based formulation, optimized for stallion (Androcoll-E™), enables a better sub-population of spermatozoa to be selected from stallion ejaculates. However, such a practice has not been critically evaluated in stallions with fertility problems. I...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169511/ https://www.ncbi.nlm.nih.gov/pubmed/30294217 http://dx.doi.org/10.1016/j.sjbs.2016.01.030 |
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author | Gamboa, Sandra Quaresma, Andreia Castro, Fátima Bravo, Pedro Rebordão, Maria Rosa Oom, Maria do Mar Rocha, António |
author_facet | Gamboa, Sandra Quaresma, Andreia Castro, Fátima Bravo, Pedro Rebordão, Maria Rosa Oom, Maria do Mar Rocha, António |
author_sort | Gamboa, Sandra |
collection | PubMed |
description | A colloid with a species specific silane-coated, silica-based formulation, optimized for stallion (Androcoll-E™), enables a better sub-population of spermatozoa to be selected from stallion ejaculates. However, such a practice has not been critically evaluated in stallions with fertility problems. In this study we evaluate whether single-layer centrifugation (SLC) through Androcoll-E™ could be used to enhance fertility rates in a subfertile stallion. Ejaculates were obtained from two different stallions, one Lusitano (fertile) and one Sorraia (subfertile), with distinct sperm characteristics and fertility. Motility, morphology, plasma membrane structural (eosin-nigrosin) and functional integrity (HOS test), mitochondrial functionality (Δψm; JC-1) and longevity (motility after 72 h cooling) after centrifugation in Androcoll-E™, as well as pregnancy rates obtained after artificial insemination (AI), with and without (control group) SLC-treated sperm were assessed. The effect of SLC on sperm characteristics, and fertility results were evaluated by ANOVA and Fisher procedures, respectively. Our results showed that SLC-selected sperm did not differ from the raw semen in terms of viability, morphology, response to hypo-osmotic conditions (HOS test) and mitochondrial membrane potential (↑ΔΨmit; JC-1). Sperm motility in cooled samples was not improved by SLC treatment. Our data show that SLC through Androcoll-E™ has no effect on pregnancy rates in the stallions used in this trial. |
format | Online Article Text |
id | pubmed-6169511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61695112018-10-05 In vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E™ Gamboa, Sandra Quaresma, Andreia Castro, Fátima Bravo, Pedro Rebordão, Maria Rosa Oom, Maria do Mar Rocha, António Saudi J Biol Sci Article A colloid with a species specific silane-coated, silica-based formulation, optimized for stallion (Androcoll-E™), enables a better sub-population of spermatozoa to be selected from stallion ejaculates. However, such a practice has not been critically evaluated in stallions with fertility problems. In this study we evaluate whether single-layer centrifugation (SLC) through Androcoll-E™ could be used to enhance fertility rates in a subfertile stallion. Ejaculates were obtained from two different stallions, one Lusitano (fertile) and one Sorraia (subfertile), with distinct sperm characteristics and fertility. Motility, morphology, plasma membrane structural (eosin-nigrosin) and functional integrity (HOS test), mitochondrial functionality (Δψm; JC-1) and longevity (motility after 72 h cooling) after centrifugation in Androcoll-E™, as well as pregnancy rates obtained after artificial insemination (AI), with and without (control group) SLC-treated sperm were assessed. The effect of SLC on sperm characteristics, and fertility results were evaluated by ANOVA and Fisher procedures, respectively. Our results showed that SLC-selected sperm did not differ from the raw semen in terms of viability, morphology, response to hypo-osmotic conditions (HOS test) and mitochondrial membrane potential (↑ΔΨmit; JC-1). Sperm motility in cooled samples was not improved by SLC treatment. Our data show that SLC through Androcoll-E™ has no effect on pregnancy rates in the stallions used in this trial. Elsevier 2017-11 2016-01-22 /pmc/articles/PMC6169511/ /pubmed/30294217 http://dx.doi.org/10.1016/j.sjbs.2016.01.030 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Gamboa, Sandra Quaresma, Andreia Castro, Fátima Bravo, Pedro Rebordão, Maria Rosa Oom, Maria do Mar Rocha, António In vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E™ |
title | In vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E™ |
title_full | In vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E™ |
title_fullStr | In vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E™ |
title_full_unstemmed | In vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E™ |
title_short | In vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E™ |
title_sort | in vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with androcoll-e™ |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169511/ https://www.ncbi.nlm.nih.gov/pubmed/30294217 http://dx.doi.org/10.1016/j.sjbs.2016.01.030 |
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