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Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator

The selection of probiotic strains resistant to gastrointestinal transit is an important stage when developing supplements that contain viable biomass. A total of six strains belonging to different genotypes were tested and compared with both a positive and negative control (Lactobacillus plantarum...

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Autor principal: Vamanu, Emanuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169548/
https://www.ncbi.nlm.nih.gov/pubmed/30294212
http://dx.doi.org/10.1016/j.sjbs.2015.06.028
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author Vamanu, Emanuel
author_facet Vamanu, Emanuel
author_sort Vamanu, Emanuel
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description The selection of probiotic strains resistant to gastrointestinal transit is an important stage when developing supplements that contain viable biomass. A total of six strains belonging to different genotypes were tested and compared with both a positive and negative control (Lactobacillus plantarum 5s). Significant differences were found between strains as a result of gastrointestinal transit using the in vitro GIS1 static simulator. The Lactobacillus rhamnosus 428ST strain showed maximum viability as a result of in vitro transit, featuring a survival capacity value, Cs, of over 50 ± 0.01%. The remaining genotypes that were tested showed significant reductions in the enzymes and bile salts at the time of action. The value of the survivability capacity was directly correlated with the synthesis of exopolysaccharides and lactic acid. The test results of the GIS1 system have been compared with those of other studies on gastrointestinal transit resistance that used dynamic models.
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spelling pubmed-61695482018-10-05 Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator Vamanu, Emanuel Saudi J Biol Sci Article The selection of probiotic strains resistant to gastrointestinal transit is an important stage when developing supplements that contain viable biomass. A total of six strains belonging to different genotypes were tested and compared with both a positive and negative control (Lactobacillus plantarum 5s). Significant differences were found between strains as a result of gastrointestinal transit using the in vitro GIS1 static simulator. The Lactobacillus rhamnosus 428ST strain showed maximum viability as a result of in vitro transit, featuring a survival capacity value, Cs, of over 50 ± 0.01%. The remaining genotypes that were tested showed significant reductions in the enzymes and bile salts at the time of action. The value of the survivability capacity was directly correlated with the synthesis of exopolysaccharides and lactic acid. The test results of the GIS1 system have been compared with those of other studies on gastrointestinal transit resistance that used dynamic models. Elsevier 2017-11 2015-07-04 /pmc/articles/PMC6169548/ /pubmed/30294212 http://dx.doi.org/10.1016/j.sjbs.2015.06.028 Text en © 2015 The Author http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Vamanu, Emanuel
Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator
title Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator
title_full Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator
title_fullStr Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator
title_full_unstemmed Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator
title_short Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator
title_sort effect of gastric and small intestinal digestion on lactic acid bacteria activity in a gis1 simulator
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169548/
https://www.ncbi.nlm.nih.gov/pubmed/30294212
http://dx.doi.org/10.1016/j.sjbs.2015.06.028
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