Cargando…
Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics
The CNS of the protovertebrate Ciona intestinalis contains a single cluster of dopaminergic (DA) neurons, the coronet cells, which have been likened to the hypothalamus of vertebrates. Whole-embryo single-cell RNA sequencing (RNA-seq) assays identified Ptf1a as the most strongly expressed cell-speci...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169837/ https://www.ncbi.nlm.nih.gov/pubmed/30228204 http://dx.doi.org/10.1101/gad.317669.118 |
_version_ | 1783360565906767872 |
---|---|
author | Horie, Takeo Horie, Ryoko Chen, Kai Cao, Chen Nakagawa, Masashi Kusakabe, Takehiro G. Satoh, Noriyuki Sasakura, Yasunori Levine, Michael |
author_facet | Horie, Takeo Horie, Ryoko Chen, Kai Cao, Chen Nakagawa, Masashi Kusakabe, Takehiro G. Satoh, Noriyuki Sasakura, Yasunori Levine, Michael |
author_sort | Horie, Takeo |
collection | PubMed |
description | The CNS of the protovertebrate Ciona intestinalis contains a single cluster of dopaminergic (DA) neurons, the coronet cells, which have been likened to the hypothalamus of vertebrates. Whole-embryo single-cell RNA sequencing (RNA-seq) assays identified Ptf1a as the most strongly expressed cell-specific transcription factor (TF) in DA/coronet cells. Knockdown of Ptf1a activity results in their loss, while misexpression results in the appearance of supernumerary DA/coronet cells. Photoreceptor cells and ependymal cells are the most susceptible to transformation, and both cell types express high levels of Meis. Coexpression of both Ptf1a and Meis caused the wholesale transformation of the entire CNS into DA/coronet cells. We therefore suggest that the reiterative use of functional manipulations and single-cell RNA-seq assays is an effective means for the identification of regulatory cocktails underlying the specification of specific cell identities. |
format | Online Article Text |
id | pubmed-6169837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-61698372019-04-01 Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics Horie, Takeo Horie, Ryoko Chen, Kai Cao, Chen Nakagawa, Masashi Kusakabe, Takehiro G. Satoh, Noriyuki Sasakura, Yasunori Levine, Michael Genes Dev Research Communication The CNS of the protovertebrate Ciona intestinalis contains a single cluster of dopaminergic (DA) neurons, the coronet cells, which have been likened to the hypothalamus of vertebrates. Whole-embryo single-cell RNA sequencing (RNA-seq) assays identified Ptf1a as the most strongly expressed cell-specific transcription factor (TF) in DA/coronet cells. Knockdown of Ptf1a activity results in their loss, while misexpression results in the appearance of supernumerary DA/coronet cells. Photoreceptor cells and ependymal cells are the most susceptible to transformation, and both cell types express high levels of Meis. Coexpression of both Ptf1a and Meis caused the wholesale transformation of the entire CNS into DA/coronet cells. We therefore suggest that the reiterative use of functional manipulations and single-cell RNA-seq assays is an effective means for the identification of regulatory cocktails underlying the specification of specific cell identities. Cold Spring Harbor Laboratory Press 2018-10-01 /pmc/articles/PMC6169837/ /pubmed/30228204 http://dx.doi.org/10.1101/gad.317669.118 Text en © 2018 Horie et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Communication Horie, Takeo Horie, Ryoko Chen, Kai Cao, Chen Nakagawa, Masashi Kusakabe, Takehiro G. Satoh, Noriyuki Sasakura, Yasunori Levine, Michael Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics |
title | Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics |
title_full | Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics |
title_fullStr | Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics |
title_full_unstemmed | Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics |
title_short | Regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics |
title_sort | regulatory cocktail for dopaminergic neurons in a protovertebrate identified by whole-embryo single-cell transcriptomics |
topic | Research Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169837/ https://www.ncbi.nlm.nih.gov/pubmed/30228204 http://dx.doi.org/10.1101/gad.317669.118 |
work_keys_str_mv | AT horietakeo regulatorycocktailfordopaminergicneuronsinaprotovertebrateidentifiedbywholeembryosinglecelltranscriptomics AT horieryoko regulatorycocktailfordopaminergicneuronsinaprotovertebrateidentifiedbywholeembryosinglecelltranscriptomics AT chenkai regulatorycocktailfordopaminergicneuronsinaprotovertebrateidentifiedbywholeembryosinglecelltranscriptomics AT caochen regulatorycocktailfordopaminergicneuronsinaprotovertebrateidentifiedbywholeembryosinglecelltranscriptomics AT nakagawamasashi regulatorycocktailfordopaminergicneuronsinaprotovertebrateidentifiedbywholeembryosinglecelltranscriptomics AT kusakabetakehirog regulatorycocktailfordopaminergicneuronsinaprotovertebrateidentifiedbywholeembryosinglecelltranscriptomics AT satohnoriyuki regulatorycocktailfordopaminergicneuronsinaprotovertebrateidentifiedbywholeembryosinglecelltranscriptomics AT sasakurayasunori regulatorycocktailfordopaminergicneuronsinaprotovertebrateidentifiedbywholeembryosinglecelltranscriptomics AT levinemichael regulatorycocktailfordopaminergicneuronsinaprotovertebrateidentifiedbywholeembryosinglecelltranscriptomics |