Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon

Arboviruses are a major public health problem worldwide and are predominantly present in intertropical areas. Chikungunya, dengue and zika viruses have been implicated in recent epidemics in Asia, America and Africa. In Cameroon, data on these viruses are fragmentary. The purpose of this study was t...

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Autores principales: Yousseu, Francine Berlange Sado, Nemg, Fredy Brice Simo, Ngouanet, Sandra Ateutchia, Mekanda, Franck Martin Obam, Demanou, Maurice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169880/
https://www.ncbi.nlm.nih.gov/pubmed/30281633
http://dx.doi.org/10.1371/journal.pone.0204143
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author Yousseu, Francine Berlange Sado
Nemg, Fredy Brice Simo
Ngouanet, Sandra Ateutchia
Mekanda, Franck Martin Obam
Demanou, Maurice
author_facet Yousseu, Francine Berlange Sado
Nemg, Fredy Brice Simo
Ngouanet, Sandra Ateutchia
Mekanda, Franck Martin Obam
Demanou, Maurice
author_sort Yousseu, Francine Berlange Sado
collection PubMed
description Arboviruses are a major public health problem worldwide and are predominantly present in intertropical areas. Chikungunya, dengue and zika viruses have been implicated in recent epidemics in Asia, America and Africa. In Cameroon, data on these viruses are fragmentary. The purpose of this study was to determine the frequency of detection of these three viruses in febrile patients in Douala, Cameroon. A cross-sectional and descriptive study was conducted from March to April 2017 at the New-Bell District Hospital in Douala. Blood samples were collected from febrile patients and tested for malaria infections using Rapid Diagnostic test. Plasma harvested was later analyzed for the presence of chikungunya, dengue and zika viruses by a Trioplex real-time RT-PCR at Centre Pasteur of Cameroon. A total of 114 participants were included, of which 63.2% were females, reflecting a sex ratio (female/male) of 1.7. The median age was 26 years, range [0.25–81]. Eight (7%) of the 114 participants were infected with Dengue virus (DENV) among which 5 were identified as serotype 1. No cases of infection by either Zika virus or Chikungunya virus were detected. Three cases of dengue-malaria co-infection (13%) were recorded. No association was found between socio-demographic factors and dengue infection. The phylogenetic analysis of the partial envelope E gene showed that all the five DENV serotype 1 samples belonged to subtype V, similarly to strains from West African countries, particularly those from Nigeria, Senegal and Côte d’Ivoire. This study showed the circulation of DENV serotype 1 in febrile patients and raises the alarm for the establishment of a sustained surveillance system to detect cases and prevent potential outbreaks in Cameroon. The existence of dengue-malaria co-infections suggests that surveillance of arboviruses should not be limited to febrile, non-malarial cases.
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spelling pubmed-61698802018-10-19 Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon Yousseu, Francine Berlange Sado Nemg, Fredy Brice Simo Ngouanet, Sandra Ateutchia Mekanda, Franck Martin Obam Demanou, Maurice PLoS One Research Article Arboviruses are a major public health problem worldwide and are predominantly present in intertropical areas. Chikungunya, dengue and zika viruses have been implicated in recent epidemics in Asia, America and Africa. In Cameroon, data on these viruses are fragmentary. The purpose of this study was to determine the frequency of detection of these three viruses in febrile patients in Douala, Cameroon. A cross-sectional and descriptive study was conducted from March to April 2017 at the New-Bell District Hospital in Douala. Blood samples were collected from febrile patients and tested for malaria infections using Rapid Diagnostic test. Plasma harvested was later analyzed for the presence of chikungunya, dengue and zika viruses by a Trioplex real-time RT-PCR at Centre Pasteur of Cameroon. A total of 114 participants were included, of which 63.2% were females, reflecting a sex ratio (female/male) of 1.7. The median age was 26 years, range [0.25–81]. Eight (7%) of the 114 participants were infected with Dengue virus (DENV) among which 5 were identified as serotype 1. No cases of infection by either Zika virus or Chikungunya virus were detected. Three cases of dengue-malaria co-infection (13%) were recorded. No association was found between socio-demographic factors and dengue infection. The phylogenetic analysis of the partial envelope E gene showed that all the five DENV serotype 1 samples belonged to subtype V, similarly to strains from West African countries, particularly those from Nigeria, Senegal and Côte d’Ivoire. This study showed the circulation of DENV serotype 1 in febrile patients and raises the alarm for the establishment of a sustained surveillance system to detect cases and prevent potential outbreaks in Cameroon. The existence of dengue-malaria co-infections suggests that surveillance of arboviruses should not be limited to febrile, non-malarial cases. Public Library of Science 2018-10-03 /pmc/articles/PMC6169880/ /pubmed/30281633 http://dx.doi.org/10.1371/journal.pone.0204143 Text en © 2018 Yousseu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yousseu, Francine Berlange Sado
Nemg, Fredy Brice Simo
Ngouanet, Sandra Ateutchia
Mekanda, Franck Martin Obam
Demanou, Maurice
Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon
title Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon
title_full Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon
title_fullStr Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon
title_full_unstemmed Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon
title_short Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon
title_sort detection and serotyping of dengue viruses in febrile patients consulting at the new-bell district hospital in douala, cameroon
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169880/
https://www.ncbi.nlm.nih.gov/pubmed/30281633
http://dx.doi.org/10.1371/journal.pone.0204143
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