Targeted genome fragmentation with CRISPR/Cas9 enables fast and efficient enrichment of small genomic regions and ultra-accurate sequencing with low DNA input (CRISPR-DS)

Next-generation sequencing methods suffer from low recovery, uneven coverage, and false mutations. DNA fragmentation by sonication is a major contributor to these problems because it produces randomly sized fragments, PCR amplification bias, and end artifacts. In addition, oligonucleotide-based hybr...

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Detalles Bibliográficos
Autores principales: Nachmanson, Daniela, Lian, Shenyi, Schmidt, Elizabeth K., Hipp, Michael J., Baker, Kathryn T., Zhang, Yuezheng, Tretiakova, Maria, Loubet-Senear, Kaitlyn, Kohrn, Brendan F., Salk, Jesse J., Kennedy, Scott R., Risques, Rosa Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169890/
https://www.ncbi.nlm.nih.gov/pubmed/30232196
http://dx.doi.org/10.1101/gr.235291.118

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