One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination...

Descripción completa

Detalles Bibliográficos
Autores principales: Jacobsen, Johanne T., Mesin, Luka, Markoulaki, Styliani, Schiepers, Ariën, Cavazzoni, Cecília B., Bousbaine, Djenet, Jaenisch, Rudolf, Victora, Gabriel D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2018
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170169/
https://www.ncbi.nlm.nih.gov/pubmed/30181412
http://dx.doi.org/10.1084/jem.20172064
Descripción
Sumario:We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation.

Ejemplares similares