One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination...

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Autores principales: Jacobsen, Johanne T., Mesin, Luka, Markoulaki, Styliani, Schiepers, Ariën, Cavazzoni, Cecília B., Bousbaine, Djenet, Jaenisch, Rudolf, Victora, Gabriel D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2018
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170169/
https://www.ncbi.nlm.nih.gov/pubmed/30181412
http://dx.doi.org/10.1084/jem.20172064
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author Jacobsen, Johanne T.
Mesin, Luka
Markoulaki, Styliani
Schiepers, Ariën
Cavazzoni, Cecília B.
Bousbaine, Djenet
Jaenisch, Rudolf
Victora, Gabriel D.
author_facet Jacobsen, Johanne T.
Mesin, Luka
Markoulaki, Styliani
Schiepers, Ariën
Cavazzoni, Cecília B.
Bousbaine, Djenet
Jaenisch, Rudolf
Victora, Gabriel D.
author_sort Jacobsen, Johanne T.
collection PubMed
description We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation.
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spelling pubmed-61701692019-04-01 One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation Jacobsen, Johanne T. Mesin, Luka Markoulaki, Styliani Schiepers, Ariën Cavazzoni, Cecília B. Bousbaine, Djenet Jaenisch, Rudolf Victora, Gabriel D. J Exp Med Research Articles We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation. Rockefeller University Press 2018-10-01 /pmc/articles/PMC6170169/ /pubmed/30181412 http://dx.doi.org/10.1084/jem.20172064 Text en © 2018 Jacobsen et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Jacobsen, Johanne T.
Mesin, Luka
Markoulaki, Styliani
Schiepers, Ariën
Cavazzoni, Cecília B.
Bousbaine, Djenet
Jaenisch, Rudolf
Victora, Gabriel D.
One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
title One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
title_full One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
title_fullStr One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
title_full_unstemmed One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
title_short One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
title_sort one-step generation of monoclonal b cell receptor mice capable of isotype switching and somatic hypermutation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170169/
https://www.ncbi.nlm.nih.gov/pubmed/30181412
http://dx.doi.org/10.1084/jem.20172064
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