Fast Dynamic in vivo Monitoring of Erk Activity at Single Cell Resolution in DREKA Zebrafish

Precise regulation of signaling pathways in single cells underlies tissue development, maintenance and repair in multicellular organisms, but our ability to monitor signaling dynamics in living vertebrates is currently limited. We implemented kinase translocation reporter (KTR) technology to create...

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Autores principales: Mayr, Vanessa, Sturtzel, Caterina, Stadler, Manuela, Grissenberger, Sarah, Distel, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170801/
https://www.ncbi.nlm.nih.gov/pubmed/30320107
http://dx.doi.org/10.3389/fcell.2018.00111
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author Mayr, Vanessa
Sturtzel, Caterina
Stadler, Manuela
Grissenberger, Sarah
Distel, Martin
author_facet Mayr, Vanessa
Sturtzel, Caterina
Stadler, Manuela
Grissenberger, Sarah
Distel, Martin
author_sort Mayr, Vanessa
collection PubMed
description Precise regulation of signaling pathways in single cells underlies tissue development, maintenance and repair in multicellular organisms, but our ability to monitor signaling dynamics in living vertebrates is currently limited. We implemented kinase translocation reporter (KTR) technology to create DREKA (“dynamic reporter of Erk activity”) zebrafish, which allow one to observe Erk activity in vivo at single cell level with high temporal resolution. DREKA zebrafish faithfully reported Erk activity after muscle cell wounding and revealed the kinetics of small compound uptake. Our results promise that kinase translocation reporters can be adapted for further applications in developmental biology, disease modeling, and in vivo pharmacology in zebrafish.
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spelling pubmed-61708012018-10-12 Fast Dynamic in vivo Monitoring of Erk Activity at Single Cell Resolution in DREKA Zebrafish Mayr, Vanessa Sturtzel, Caterina Stadler, Manuela Grissenberger, Sarah Distel, Martin Front Cell Dev Biol Cell and Developmental Biology Precise regulation of signaling pathways in single cells underlies tissue development, maintenance and repair in multicellular organisms, but our ability to monitor signaling dynamics in living vertebrates is currently limited. We implemented kinase translocation reporter (KTR) technology to create DREKA (“dynamic reporter of Erk activity”) zebrafish, which allow one to observe Erk activity in vivo at single cell level with high temporal resolution. DREKA zebrafish faithfully reported Erk activity after muscle cell wounding and revealed the kinetics of small compound uptake. Our results promise that kinase translocation reporters can be adapted for further applications in developmental biology, disease modeling, and in vivo pharmacology in zebrafish. Frontiers Media S.A. 2018-09-25 /pmc/articles/PMC6170801/ /pubmed/30320107 http://dx.doi.org/10.3389/fcell.2018.00111 Text en Copyright © 2018 Mayr, Sturtzel, Stadler, Grissenberger and Distel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Mayr, Vanessa
Sturtzel, Caterina
Stadler, Manuela
Grissenberger, Sarah
Distel, Martin
Fast Dynamic in vivo Monitoring of Erk Activity at Single Cell Resolution in DREKA Zebrafish
title Fast Dynamic in vivo Monitoring of Erk Activity at Single Cell Resolution in DREKA Zebrafish
title_full Fast Dynamic in vivo Monitoring of Erk Activity at Single Cell Resolution in DREKA Zebrafish
title_fullStr Fast Dynamic in vivo Monitoring of Erk Activity at Single Cell Resolution in DREKA Zebrafish
title_full_unstemmed Fast Dynamic in vivo Monitoring of Erk Activity at Single Cell Resolution in DREKA Zebrafish
title_short Fast Dynamic in vivo Monitoring of Erk Activity at Single Cell Resolution in DREKA Zebrafish
title_sort fast dynamic in vivo monitoring of erk activity at single cell resolution in dreka zebrafish
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170801/
https://www.ncbi.nlm.nih.gov/pubmed/30320107
http://dx.doi.org/10.3389/fcell.2018.00111
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