Functional validation of human-specific PowerPlex(®) 21 System (Promega, USA) in chimpanzee (Pan troglodytes)

OBJECTIVE: This study was aimed to test the PowerPlex(®) 21 System (Promega, USA), used for human identification applications for its positive cross-species applicability in Chimpanzees (Pan troglodytes) in order to identify heterologous STRs which can be used for individual identification, paternit...

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Autores principales: Thakur, Mukesh, Chandra, Kailash, Sahajpal, Vivek, Samanta, Asis, Sharma, Arun, Mitra, Agni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171178/
https://www.ncbi.nlm.nih.gov/pubmed/30285836
http://dx.doi.org/10.1186/s13104-018-3803-x
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author Thakur, Mukesh
Chandra, Kailash
Sahajpal, Vivek
Samanta, Asis
Sharma, Arun
Mitra, Agni
author_facet Thakur, Mukesh
Chandra, Kailash
Sahajpal, Vivek
Samanta, Asis
Sharma, Arun
Mitra, Agni
author_sort Thakur, Mukesh
collection PubMed
description OBJECTIVE: This study was aimed to test the PowerPlex(®) 21 System (Promega, USA), used for human identification applications for its positive cross-species applicability in Chimpanzees (Pan troglodytes) in order to identify heterologous STRs which can be used for individual identification, paternity testing, relatedness establishment and reconstruction of pedigrees and studbook records for captive and wild chimpanzee breeding populations. RESULTS: Of 21 STRs in PowerPlex(®) 21 System (Promega, USA), 19 loci amplified and found to be polymorphic. Locus Aml showed differential banding patterns in males and females similar to those seen for humans and correctly assigned sexes of known identity. Altogether, 58 different alleles were found with an average 3.05 ± 0.28 alleles per locus. Mean observed (Ho), and expected heterozygosity (He) were 0.93 ± 0.03 and 0.52 ± 0.05, respectively.
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spelling pubmed-61711782018-10-10 Functional validation of human-specific PowerPlex(®) 21 System (Promega, USA) in chimpanzee (Pan troglodytes) Thakur, Mukesh Chandra, Kailash Sahajpal, Vivek Samanta, Asis Sharma, Arun Mitra, Agni BMC Res Notes Research Note OBJECTIVE: This study was aimed to test the PowerPlex(®) 21 System (Promega, USA), used for human identification applications for its positive cross-species applicability in Chimpanzees (Pan troglodytes) in order to identify heterologous STRs which can be used for individual identification, paternity testing, relatedness establishment and reconstruction of pedigrees and studbook records for captive and wild chimpanzee breeding populations. RESULTS: Of 21 STRs in PowerPlex(®) 21 System (Promega, USA), 19 loci amplified and found to be polymorphic. Locus Aml showed differential banding patterns in males and females similar to those seen for humans and correctly assigned sexes of known identity. Altogether, 58 different alleles were found with an average 3.05 ± 0.28 alleles per locus. Mean observed (Ho), and expected heterozygosity (He) were 0.93 ± 0.03 and 0.52 ± 0.05, respectively. BioMed Central 2018-10-03 /pmc/articles/PMC6171178/ /pubmed/30285836 http://dx.doi.org/10.1186/s13104-018-3803-x Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Thakur, Mukesh
Chandra, Kailash
Sahajpal, Vivek
Samanta, Asis
Sharma, Arun
Mitra, Agni
Functional validation of human-specific PowerPlex(®) 21 System (Promega, USA) in chimpanzee (Pan troglodytes)
title Functional validation of human-specific PowerPlex(®) 21 System (Promega, USA) in chimpanzee (Pan troglodytes)
title_full Functional validation of human-specific PowerPlex(®) 21 System (Promega, USA) in chimpanzee (Pan troglodytes)
title_fullStr Functional validation of human-specific PowerPlex(®) 21 System (Promega, USA) in chimpanzee (Pan troglodytes)
title_full_unstemmed Functional validation of human-specific PowerPlex(®) 21 System (Promega, USA) in chimpanzee (Pan troglodytes)
title_short Functional validation of human-specific PowerPlex(®) 21 System (Promega, USA) in chimpanzee (Pan troglodytes)
title_sort functional validation of human-specific powerplex(®) 21 system (promega, usa) in chimpanzee (pan troglodytes)
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171178/
https://www.ncbi.nlm.nih.gov/pubmed/30285836
http://dx.doi.org/10.1186/s13104-018-3803-x
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