The ADRP domain from a virulent strain of infectious bronchitis virus is not sufficient to confer a pathogenic phenotype to the attenuated Beaudette strain
The replicase gene of the coronavirus infectious bronchitis virus (IBV) encodes 15 non-structural proteins (nsps). Nsp 3 is a multi-functional protein containing a conserved ADP-ribose-1″-phosphatase (ADRP) domain. The crystal structures of the domain from two strains of IBV, M41 (virulent) and Beau...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171709/ https://www.ncbi.nlm.nih.gov/pubmed/29893665 http://dx.doi.org/10.1099/jgv.0.001098 |
_version_ | 1783360814211661824 |
---|---|
author | Keep, Sarah Bickerton, Erica Armesto, Maria Britton, Paul |
author_facet | Keep, Sarah Bickerton, Erica Armesto, Maria Britton, Paul |
author_sort | Keep, Sarah |
collection | PubMed |
description | The replicase gene of the coronavirus infectious bronchitis virus (IBV) encodes 15 non-structural proteins (nsps). Nsp 3 is a multi-functional protein containing a conserved ADP-ribose-1″-phosphatase (ADRP) domain. The crystal structures of the domain from two strains of IBV, M41 (virulent) and Beaudette (avirulent), identified a key difference; M41 contains a conserved triple-glycine motif, whilst Beaudette contains a glycine-to-serine mutation that is predicted to abolish ADRP activity. Although ADRP activity has not been formally demonstrated for IBV nsp 3, Beaudette fails to bind ADP-ribose. The role of ADRP in virulence was investigated by generating rIBVs, based on Beaudette, containing either a restored triple-glycine motif or the complete M41 ADRP domain. Replication in vitro was unaffected by the ADRP modifications and the in vivo phenotype of the rIBVs was found to be apathogenic, indicating that restoration of the triple-glycine motif is not sufficient to restore virulence to the apathogenic Beaudette strain. |
format | Online Article Text |
id | pubmed-6171709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-61717092018-10-09 The ADRP domain from a virulent strain of infectious bronchitis virus is not sufficient to confer a pathogenic phenotype to the attenuated Beaudette strain Keep, Sarah Bickerton, Erica Armesto, Maria Britton, Paul J Gen Virol Short Communication The replicase gene of the coronavirus infectious bronchitis virus (IBV) encodes 15 non-structural proteins (nsps). Nsp 3 is a multi-functional protein containing a conserved ADP-ribose-1″-phosphatase (ADRP) domain. The crystal structures of the domain from two strains of IBV, M41 (virulent) and Beaudette (avirulent), identified a key difference; M41 contains a conserved triple-glycine motif, whilst Beaudette contains a glycine-to-serine mutation that is predicted to abolish ADRP activity. Although ADRP activity has not been formally demonstrated for IBV nsp 3, Beaudette fails to bind ADP-ribose. The role of ADRP in virulence was investigated by generating rIBVs, based on Beaudette, containing either a restored triple-glycine motif or the complete M41 ADRP domain. Replication in vitro was unaffected by the ADRP modifications and the in vivo phenotype of the rIBVs was found to be apathogenic, indicating that restoration of the triple-glycine motif is not sufficient to restore virulence to the apathogenic Beaudette strain. Microbiology Society 2018-08 2018-06-12 /pmc/articles/PMC6171709/ /pubmed/29893665 http://dx.doi.org/10.1099/jgv.0.001098 Text en © 2018 The Authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Keep, Sarah Bickerton, Erica Armesto, Maria Britton, Paul The ADRP domain from a virulent strain of infectious bronchitis virus is not sufficient to confer a pathogenic phenotype to the attenuated Beaudette strain |
title | The ADRP domain from a virulent strain of infectious bronchitis virus is not sufficient to confer a pathogenic phenotype to the attenuated Beaudette strain |
title_full | The ADRP domain from a virulent strain of infectious bronchitis virus is not sufficient to confer a pathogenic phenotype to the attenuated Beaudette strain |
title_fullStr | The ADRP domain from a virulent strain of infectious bronchitis virus is not sufficient to confer a pathogenic phenotype to the attenuated Beaudette strain |
title_full_unstemmed | The ADRP domain from a virulent strain of infectious bronchitis virus is not sufficient to confer a pathogenic phenotype to the attenuated Beaudette strain |
title_short | The ADRP domain from a virulent strain of infectious bronchitis virus is not sufficient to confer a pathogenic phenotype to the attenuated Beaudette strain |
title_sort | adrp domain from a virulent strain of infectious bronchitis virus is not sufficient to confer a pathogenic phenotype to the attenuated beaudette strain |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171709/ https://www.ncbi.nlm.nih.gov/pubmed/29893665 http://dx.doi.org/10.1099/jgv.0.001098 |
work_keys_str_mv | AT keepsarah theadrpdomainfromavirulentstrainofinfectiousbronchitisvirusisnotsufficienttoconferapathogenicphenotypetotheattenuatedbeaudettestrain AT bickertonerica theadrpdomainfromavirulentstrainofinfectiousbronchitisvirusisnotsufficienttoconferapathogenicphenotypetotheattenuatedbeaudettestrain AT armestomaria theadrpdomainfromavirulentstrainofinfectiousbronchitisvirusisnotsufficienttoconferapathogenicphenotypetotheattenuatedbeaudettestrain AT brittonpaul theadrpdomainfromavirulentstrainofinfectiousbronchitisvirusisnotsufficienttoconferapathogenicphenotypetotheattenuatedbeaudettestrain AT keepsarah adrpdomainfromavirulentstrainofinfectiousbronchitisvirusisnotsufficienttoconferapathogenicphenotypetotheattenuatedbeaudettestrain AT bickertonerica adrpdomainfromavirulentstrainofinfectiousbronchitisvirusisnotsufficienttoconferapathogenicphenotypetotheattenuatedbeaudettestrain AT armestomaria adrpdomainfromavirulentstrainofinfectiousbronchitisvirusisnotsufficienttoconferapathogenicphenotypetotheattenuatedbeaudettestrain AT brittonpaul adrpdomainfromavirulentstrainofinfectiousbronchitisvirusisnotsufficienttoconferapathogenicphenotypetotheattenuatedbeaudettestrain |