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Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer

Background: Toll-like receptor 9 (TLR9) plays a key role in the elimination of viral pathogens by recognising their CpG DNA. Polymorphisms in the TLR9 gene may influence their recognition and subsequent elimination. Therefore, the present study was designed to elucidate the role of a rare unexplored...

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Autores principales: Chauhan, Alex, Pandey, Nilesh, Raithatha, Nitin, Patel, Purvi, Desai, Ajesh, Jain, Neeraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171715/
https://www.ncbi.nlm.nih.gov/pubmed/30345020
http://dx.doi.org/10.12688/f1000research.14840.2
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author Chauhan, Alex
Pandey, Nilesh
Raithatha, Nitin
Patel, Purvi
Desai, Ajesh
Jain, Neeraj
author_facet Chauhan, Alex
Pandey, Nilesh
Raithatha, Nitin
Patel, Purvi
Desai, Ajesh
Jain, Neeraj
author_sort Chauhan, Alex
collection PubMed
description Background: Toll-like receptor 9 (TLR9) plays a key role in the elimination of viral pathogens by recognising their CpG DNA. Polymorphisms in the TLR9 gene may influence their recognition and subsequent elimination. Therefore, the present study was designed to elucidate the role of a rare unexplored TLR9 gene polymorphism C296T/ Pro99Leu (rs5743844) in cervical cancer susceptibility among Indian women. Methods: The genotyping of TLR9 Pro99Leu polymorphism in 110 cervical cancer patients and 141 healthy controls was performed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results: The genotype frequency detected in both cervical cancer and control populations was 1.0 (CC), 0.0 (CT) and 0.0 (TT); while the allele frequency was found to be 1.0 (C) and 0.0 (T). Conclusions: The present study demonstrates no involvement of TLR9 C296T/ Pro99Leu polymorphism in cervical cancer susceptibility and supports minor allele frequency (MAF) (0.0002) status of the same as no nucleotide variation was detected in any of the study subjects.
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spelling pubmed-61717152018-10-19 Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer Chauhan, Alex Pandey, Nilesh Raithatha, Nitin Patel, Purvi Desai, Ajesh Jain, Neeraj F1000Res Research Article Background: Toll-like receptor 9 (TLR9) plays a key role in the elimination of viral pathogens by recognising their CpG DNA. Polymorphisms in the TLR9 gene may influence their recognition and subsequent elimination. Therefore, the present study was designed to elucidate the role of a rare unexplored TLR9 gene polymorphism C296T/ Pro99Leu (rs5743844) in cervical cancer susceptibility among Indian women. Methods: The genotyping of TLR9 Pro99Leu polymorphism in 110 cervical cancer patients and 141 healthy controls was performed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results: The genotype frequency detected in both cervical cancer and control populations was 1.0 (CC), 0.0 (CT) and 0.0 (TT); while the allele frequency was found to be 1.0 (C) and 0.0 (T). Conclusions: The present study demonstrates no involvement of TLR9 C296T/ Pro99Leu polymorphism in cervical cancer susceptibility and supports minor allele frequency (MAF) (0.0002) status of the same as no nucleotide variation was detected in any of the study subjects. F1000 Research Limited 2018-08-30 /pmc/articles/PMC6171715/ /pubmed/30345020 http://dx.doi.org/10.12688/f1000research.14840.2 Text en Copyright: © 2018 Chauhan A et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chauhan, Alex
Pandey, Nilesh
Raithatha, Nitin
Patel, Purvi
Desai, Ajesh
Jain, Neeraj
Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer
title Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer
title_full Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer
title_fullStr Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer
title_full_unstemmed Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer
title_short Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer
title_sort absence of toll-like receptor 9 pro99leu polymorphism in cervical cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171715/
https://www.ncbi.nlm.nih.gov/pubmed/30345020
http://dx.doi.org/10.12688/f1000research.14840.2
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