Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching

[Image: see text] Stimulated emission depletion (STED) nanolithography allows nanofabrication below the diffraction limit. Recently, it was applied to nanoanchors for protein fixation down to the single molecule level. We now combined STED nanolithography with laser-assisted protein adsorption by ph...

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Autores principales: Murtezi, Eljesa, Puthukodan, Sujitha, Jacak, Jaroslaw, Klar, Thomas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171978/
https://www.ncbi.nlm.nih.gov/pubmed/30204400
http://dx.doi.org/10.1021/acsami.8b11777
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author Murtezi, Eljesa
Puthukodan, Sujitha
Jacak, Jaroslaw
Klar, Thomas A.
author_facet Murtezi, Eljesa
Puthukodan, Sujitha
Jacak, Jaroslaw
Klar, Thomas A.
author_sort Murtezi, Eljesa
collection PubMed
description [Image: see text] Stimulated emission depletion (STED) nanolithography allows nanofabrication below the diffraction limit. Recently, it was applied to nanoanchors for protein fixation down to the single molecule level. We now combined STED nanolithography with laser-assisted protein adsorption by photobleaching (LAPAP) for optical and selective attachment of proteins to subdiffractional structures. In turn, STED was used for imaging of fluorescently tagged streptavidin to reveal protein binding to STED-lithographically patterned acrylate structures via LAPAP. Protein localization down to 56 nm spots was achieved using all-optical methods at visible wavelengths.
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spelling pubmed-61719782018-10-11 Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching Murtezi, Eljesa Puthukodan, Sujitha Jacak, Jaroslaw Klar, Thomas A. ACS Appl Mater Interfaces [Image: see text] Stimulated emission depletion (STED) nanolithography allows nanofabrication below the diffraction limit. Recently, it was applied to nanoanchors for protein fixation down to the single molecule level. We now combined STED nanolithography with laser-assisted protein adsorption by photobleaching (LAPAP) for optical and selective attachment of proteins to subdiffractional structures. In turn, STED was used for imaging of fluorescently tagged streptavidin to reveal protein binding to STED-lithographically patterned acrylate structures via LAPAP. Protein localization down to 56 nm spots was achieved using all-optical methods at visible wavelengths. American Chemical Society 2018-09-11 2018-09-26 /pmc/articles/PMC6171978/ /pubmed/30204400 http://dx.doi.org/10.1021/acsami.8b11777 Text en Copyright © 2018 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Murtezi, Eljesa
Puthukodan, Sujitha
Jacak, Jaroslaw
Klar, Thomas A.
Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching
title Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching
title_full Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching
title_fullStr Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching
title_full_unstemmed Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching
title_short Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching
title_sort biofunctionalization of sub-diffractionally patterned polymer structures by photobleaching
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171978/
https://www.ncbi.nlm.nih.gov/pubmed/30204400
http://dx.doi.org/10.1021/acsami.8b11777
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AT jacakjaroslaw biofunctionalizationofsubdiffractionallypatternedpolymerstructuresbyphotobleaching
AT klarthomasa biofunctionalizationofsubdiffractionallypatternedpolymerstructuresbyphotobleaching

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