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Long noncoding RNA n339260 promotes vasculogenic mimicry and cancer stem cell development in hepatocellular carcinoma

Vasculogenic mimicry (VM) refers to the unique capability of aggressive tumor cells to mimic the pattern of embryonic vasculogenic networks. Cancer stem cells (CSC) represent a subpopulation of tumor cells endowed with the capacity for self‐renewal and multilineage differentiation. Previous studies...

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Autores principales: Zhao, Xiulan, Sun, Baocun, Liu, Tieju, Shao, Bing, Sun, Ran, Zhu, Dongwang, Zhang, Yanhui, Gu, Qiang, Dong, Xueyi, Liu, Fang, Zhao, Nan, Zhang, Danfang, Li, Yanlei, Meng, Jie, Gong, Wenchen, Zheng, Yanjun, Zheng, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172069/
https://www.ncbi.nlm.nih.gov/pubmed/30022558
http://dx.doi.org/10.1111/cas.13740
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author Zhao, Xiulan
Sun, Baocun
Liu, Tieju
Shao, Bing
Sun, Ran
Zhu, Dongwang
Zhang, Yanhui
Gu, Qiang
Dong, Xueyi
Liu, Fang
Zhao, Nan
Zhang, Danfang
Li, Yanlei
Meng, Jie
Gong, Wenchen
Zheng, Yanjun
Zheng, Xu
author_facet Zhao, Xiulan
Sun, Baocun
Liu, Tieju
Shao, Bing
Sun, Ran
Zhu, Dongwang
Zhang, Yanhui
Gu, Qiang
Dong, Xueyi
Liu, Fang
Zhao, Nan
Zhang, Danfang
Li, Yanlei
Meng, Jie
Gong, Wenchen
Zheng, Yanjun
Zheng, Xu
author_sort Zhao, Xiulan
collection PubMed
description Vasculogenic mimicry (VM) refers to the unique capability of aggressive tumor cells to mimic the pattern of embryonic vasculogenic networks. Cancer stem cells (CSC) represent a subpopulation of tumor cells endowed with the capacity for self‐renewal and multilineage differentiation. Previous studies have indicated that CSC may participate in the formation of VM. With the advance of high‐resolution microarrays and massively parallel sequencing technology, long noncoding RNAs (lncRNAs) are suggested to play a critical role in tumorigenesis and, in particular, the development of human hepatocellular carcinoma (HCC). Currently, no definitive relationship between lncRNA and VM formation has been described. In the current study, we demonstrated that expression of the lncRNA, n339260, is associated with CSC phenotype in HCC, and n339260 level correlated with VM, metastasis, and shorter survival time in an animal model. Overexpression of n339260 in HepG2 cells was associated with a significant increase in CSC. Additionally, the appearance of VM and vascular endothelial (VE)‐cadherin, a molecular marker of VM, was also induced by n339260 overexpression. Using a short hairpin RNA approach, n339260 was silenced in tumor cells, and knockdown of n339260 was associated with reduced VM and CSC. The results of this study indicate that n339260 promotes VM, possibly by the development of CSC. The related molecular pathways may be used as novel therapeutic targets for the inhibition of HCC angiogenesis and metastasis.
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spelling pubmed-61720692018-10-10 Long noncoding RNA n339260 promotes vasculogenic mimicry and cancer stem cell development in hepatocellular carcinoma Zhao, Xiulan Sun, Baocun Liu, Tieju Shao, Bing Sun, Ran Zhu, Dongwang Zhang, Yanhui Gu, Qiang Dong, Xueyi Liu, Fang Zhao, Nan Zhang, Danfang Li, Yanlei Meng, Jie Gong, Wenchen Zheng, Yanjun Zheng, Xu Cancer Sci Original Articles Vasculogenic mimicry (VM) refers to the unique capability of aggressive tumor cells to mimic the pattern of embryonic vasculogenic networks. Cancer stem cells (CSC) represent a subpopulation of tumor cells endowed with the capacity for self‐renewal and multilineage differentiation. Previous studies have indicated that CSC may participate in the formation of VM. With the advance of high‐resolution microarrays and massively parallel sequencing technology, long noncoding RNAs (lncRNAs) are suggested to play a critical role in tumorigenesis and, in particular, the development of human hepatocellular carcinoma (HCC). Currently, no definitive relationship between lncRNA and VM formation has been described. In the current study, we demonstrated that expression of the lncRNA, n339260, is associated with CSC phenotype in HCC, and n339260 level correlated with VM, metastasis, and shorter survival time in an animal model. Overexpression of n339260 in HepG2 cells was associated with a significant increase in CSC. Additionally, the appearance of VM and vascular endothelial (VE)‐cadherin, a molecular marker of VM, was also induced by n339260 overexpression. Using a short hairpin RNA approach, n339260 was silenced in tumor cells, and knockdown of n339260 was associated with reduced VM and CSC. The results of this study indicate that n339260 promotes VM, possibly by the development of CSC. The related molecular pathways may be used as novel therapeutic targets for the inhibition of HCC angiogenesis and metastasis. John Wiley and Sons Inc. 2018-08-14 2018-10 /pmc/articles/PMC6172069/ /pubmed/30022558 http://dx.doi.org/10.1111/cas.13740 Text en © 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Zhao, Xiulan
Sun, Baocun
Liu, Tieju
Shao, Bing
Sun, Ran
Zhu, Dongwang
Zhang, Yanhui
Gu, Qiang
Dong, Xueyi
Liu, Fang
Zhao, Nan
Zhang, Danfang
Li, Yanlei
Meng, Jie
Gong, Wenchen
Zheng, Yanjun
Zheng, Xu
Long noncoding RNA n339260 promotes vasculogenic mimicry and cancer stem cell development in hepatocellular carcinoma
title Long noncoding RNA n339260 promotes vasculogenic mimicry and cancer stem cell development in hepatocellular carcinoma
title_full Long noncoding RNA n339260 promotes vasculogenic mimicry and cancer stem cell development in hepatocellular carcinoma
title_fullStr Long noncoding RNA n339260 promotes vasculogenic mimicry and cancer stem cell development in hepatocellular carcinoma
title_full_unstemmed Long noncoding RNA n339260 promotes vasculogenic mimicry and cancer stem cell development in hepatocellular carcinoma
title_short Long noncoding RNA n339260 promotes vasculogenic mimicry and cancer stem cell development in hepatocellular carcinoma
title_sort long noncoding rna n339260 promotes vasculogenic mimicry and cancer stem cell development in hepatocellular carcinoma
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172069/
https://www.ncbi.nlm.nih.gov/pubmed/30022558
http://dx.doi.org/10.1111/cas.13740
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