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High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury
Purpose: Blood biomarkers are a useful tool to study concussion. However, their interpretation is complicated by a number of potential biological confounds, including exercise. This is particularly relevant in military and athletic settings where injury commonly occurs during physical exertion. The...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172320/ https://www.ncbi.nlm.nih.gov/pubmed/30323770 http://dx.doi.org/10.3389/fphys.2018.01367 |
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author | Di Battista, Alex P. Moes, Katherine A. Shiu, Maria Y. Hutchison, Michael G. Churchill, Nathan Thomas, Scott G. Rhind, Shawn G. |
author_facet | Di Battista, Alex P. Moes, Katherine A. Shiu, Maria Y. Hutchison, Michael G. Churchill, Nathan Thomas, Scott G. Rhind, Shawn G. |
author_sort | Di Battista, Alex P. |
collection | PubMed |
description | Purpose: Blood biomarkers are a useful tool to study concussion. However, their interpretation is complicated by a number of potential biological confounds, including exercise. This is particularly relevant in military and athletic settings where injury commonly occurs during physical exertion. The impact of high-intensity interval training (HIIT) on putative brain injury biomarkers remains under-examined. The purpose of this study was to observe the effects of HIIT on a panel of blood biomarkers associated with brain injury. Methods: Eleven healthy, recreationally active males (median age = 29.0, interquartile range = 26.0–31.5) performed HIIT on a bicycle ergometer (8-12 × 60-s intervals at 100% of peak power output, interspersed by 75-s recovery at 50 W) three times/week for 2 weeks. Peripheral blood samples were collected before and immediately after HIIT during the first and last training sessions. Plasma concentrations of s100 calcium-binding protein beta (S100B), glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), brain-derived neurotrophic factor (BDNF), neurogranin (NRGN), peroxiredoxin (PRDX)-6, creatine kinase-BB isoenzyme (CKBB), visinin-like protein (VILIP)-1, von Willebrand factor (vWF), monocyte chemoattractant protein (MCP)-1, matrix metalloproteinase (MMP)-9, and total tau (T-tau) were quantitated by high-sensitivity MULTI-SPOT(®) immunoassay, on the MesoScale Diagnostics electrochemiluminescence detection platform. Differences in biomarker concentrations in response to HIIT were evaluated by partial least squares discriminant analysis (PLSDA) within a repeated-measures bootstrapped framework. Results: Ten of 12 biomarkers were increased pre-to-post HIIT; VILIP-1 remained unchanged, and GFAP was not statistically evaluated due to insufficient detectability. After 2 weeks of HIIT, T-tau was no longer significantly elevated pre-to-post HIIT, and significant attenuation was noted in the acute responses of NRGN, PRDX-6, MMP-9, and vWF. In addition, compared to session 1, session 6 pre-exercise concentrations of NSE and VILIP-1 were significantly lower and higher, respectively. Conclusion: Blood biomarkers commonly associated with brain injury are significantly elevated in response to a single bout of HIIT. After a 2-week, six-session training protocol, this response was attenuated for some, but not all markers. While biomarkers continue to provide promise to concussion research, future studies are necessary to disentangle the common biological sequelae to both exercise and brain injury. |
format | Online Article Text |
id | pubmed-6172320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61723202018-10-15 High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury Di Battista, Alex P. Moes, Katherine A. Shiu, Maria Y. Hutchison, Michael G. Churchill, Nathan Thomas, Scott G. Rhind, Shawn G. Front Physiol Physiology Purpose: Blood biomarkers are a useful tool to study concussion. However, their interpretation is complicated by a number of potential biological confounds, including exercise. This is particularly relevant in military and athletic settings where injury commonly occurs during physical exertion. The impact of high-intensity interval training (HIIT) on putative brain injury biomarkers remains under-examined. The purpose of this study was to observe the effects of HIIT on a panel of blood biomarkers associated with brain injury. Methods: Eleven healthy, recreationally active males (median age = 29.0, interquartile range = 26.0–31.5) performed HIIT on a bicycle ergometer (8-12 × 60-s intervals at 100% of peak power output, interspersed by 75-s recovery at 50 W) three times/week for 2 weeks. Peripheral blood samples were collected before and immediately after HIIT during the first and last training sessions. Plasma concentrations of s100 calcium-binding protein beta (S100B), glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), brain-derived neurotrophic factor (BDNF), neurogranin (NRGN), peroxiredoxin (PRDX)-6, creatine kinase-BB isoenzyme (CKBB), visinin-like protein (VILIP)-1, von Willebrand factor (vWF), monocyte chemoattractant protein (MCP)-1, matrix metalloproteinase (MMP)-9, and total tau (T-tau) were quantitated by high-sensitivity MULTI-SPOT(®) immunoassay, on the MesoScale Diagnostics electrochemiluminescence detection platform. Differences in biomarker concentrations in response to HIIT were evaluated by partial least squares discriminant analysis (PLSDA) within a repeated-measures bootstrapped framework. Results: Ten of 12 biomarkers were increased pre-to-post HIIT; VILIP-1 remained unchanged, and GFAP was not statistically evaluated due to insufficient detectability. After 2 weeks of HIIT, T-tau was no longer significantly elevated pre-to-post HIIT, and significant attenuation was noted in the acute responses of NRGN, PRDX-6, MMP-9, and vWF. In addition, compared to session 1, session 6 pre-exercise concentrations of NSE and VILIP-1 were significantly lower and higher, respectively. Conclusion: Blood biomarkers commonly associated with brain injury are significantly elevated in response to a single bout of HIIT. After a 2-week, six-session training protocol, this response was attenuated for some, but not all markers. While biomarkers continue to provide promise to concussion research, future studies are necessary to disentangle the common biological sequelae to both exercise and brain injury. Frontiers Media S.A. 2018-09-28 /pmc/articles/PMC6172320/ /pubmed/30323770 http://dx.doi.org/10.3389/fphys.2018.01367 Text en Copyright © 2018 Di Battista, Moes, Shiu, Hutchison, Churchill, Thomas and Rhind. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Di Battista, Alex P. Moes, Katherine A. Shiu, Maria Y. Hutchison, Michael G. Churchill, Nathan Thomas, Scott G. Rhind, Shawn G. High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury |
title | High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury |
title_full | High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury |
title_fullStr | High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury |
title_full_unstemmed | High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury |
title_short | High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury |
title_sort | high-intensity interval training is associated with alterations in blood biomarkers related to brain injury |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172320/ https://www.ncbi.nlm.nih.gov/pubmed/30323770 http://dx.doi.org/10.3389/fphys.2018.01367 |
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