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Microbial Community and Metabolic Activity in Thiocyanate Degrading Low Temperature Microbial Fuel Cells

Thiocyanate is a toxic compound produced by the mining and metallurgy industries that needs to be remediated prior to its release into the environment. If the industry is situated at high altitudes or near the poles, economic factors require a low temperature treatment process. Microbial fuel cells...

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Autores principales: Ni, Gaofeng, Canizales, Sebastian, Broman, Elias, Simone, Domenico, Palwai, Viraja R., Lundin, Daniel, Lopez-Fernandez, Margarita, Sleutels, Tom, Dopson, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172326/
https://www.ncbi.nlm.nih.gov/pubmed/30323799
http://dx.doi.org/10.3389/fmicb.2018.02308
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author Ni, Gaofeng
Canizales, Sebastian
Broman, Elias
Simone, Domenico
Palwai, Viraja R.
Lundin, Daniel
Lopez-Fernandez, Margarita
Sleutels, Tom
Dopson, Mark
author_facet Ni, Gaofeng
Canizales, Sebastian
Broman, Elias
Simone, Domenico
Palwai, Viraja R.
Lundin, Daniel
Lopez-Fernandez, Margarita
Sleutels, Tom
Dopson, Mark
author_sort Ni, Gaofeng
collection PubMed
description Thiocyanate is a toxic compound produced by the mining and metallurgy industries that needs to be remediated prior to its release into the environment. If the industry is situated at high altitudes or near the poles, economic factors require a low temperature treatment process. Microbial fuel cells are a developing technology that have the benefits of both removing such toxic compounds while recovering electrical energy. In this study, simultaneous thiocyanate degradation and electrical current generation was demonstrated and it was suggested that extracellular electron transfer to the anode occurred. Investigation of the microbial community by 16S rRNA metatranscriptome reads supported that the anode attached and planktonic anolyte consortia were dominated by a Thiobacillus-like population. Metatranscriptomic sequencing also suggested thiocyanate degradation primarily occurred via the ‘cyanate’ degradation pathway. The generated sulfide was metabolized via sulfite and ultimately to sulfate mediated by reverse dissimilatory sulfite reductase, APS reductase, and sulfate adenylyltransferase and the released electrons were potentially transferred to the anode via soluble electron shuttles. Finally, the ammonium from thiocyanate degradation was assimilated to glutamate as nitrogen source and carbon dioxide was fixed as carbon source. This study is one of the first to demonstrate a low temperature inorganic sulfur utilizing microbial fuel cell and the first to provide evidence for pathways of thiocyanate degradation coupled to electron transfer.
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spelling pubmed-61723262018-10-15 Microbial Community and Metabolic Activity in Thiocyanate Degrading Low Temperature Microbial Fuel Cells Ni, Gaofeng Canizales, Sebastian Broman, Elias Simone, Domenico Palwai, Viraja R. Lundin, Daniel Lopez-Fernandez, Margarita Sleutels, Tom Dopson, Mark Front Microbiol Microbiology Thiocyanate is a toxic compound produced by the mining and metallurgy industries that needs to be remediated prior to its release into the environment. If the industry is situated at high altitudes or near the poles, economic factors require a low temperature treatment process. Microbial fuel cells are a developing technology that have the benefits of both removing such toxic compounds while recovering electrical energy. In this study, simultaneous thiocyanate degradation and electrical current generation was demonstrated and it was suggested that extracellular electron transfer to the anode occurred. Investigation of the microbial community by 16S rRNA metatranscriptome reads supported that the anode attached and planktonic anolyte consortia were dominated by a Thiobacillus-like population. Metatranscriptomic sequencing also suggested thiocyanate degradation primarily occurred via the ‘cyanate’ degradation pathway. The generated sulfide was metabolized via sulfite and ultimately to sulfate mediated by reverse dissimilatory sulfite reductase, APS reductase, and sulfate adenylyltransferase and the released electrons were potentially transferred to the anode via soluble electron shuttles. Finally, the ammonium from thiocyanate degradation was assimilated to glutamate as nitrogen source and carbon dioxide was fixed as carbon source. This study is one of the first to demonstrate a low temperature inorganic sulfur utilizing microbial fuel cell and the first to provide evidence for pathways of thiocyanate degradation coupled to electron transfer. Frontiers Media S.A. 2018-09-28 /pmc/articles/PMC6172326/ /pubmed/30323799 http://dx.doi.org/10.3389/fmicb.2018.02308 Text en Copyright © 2018 Ni, Canizales, Broman, Simone, Palwai, Lundin, Lopez-Fernandez, Sleutels and Dopson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ni, Gaofeng
Canizales, Sebastian
Broman, Elias
Simone, Domenico
Palwai, Viraja R.
Lundin, Daniel
Lopez-Fernandez, Margarita
Sleutels, Tom
Dopson, Mark
Microbial Community and Metabolic Activity in Thiocyanate Degrading Low Temperature Microbial Fuel Cells
title Microbial Community and Metabolic Activity in Thiocyanate Degrading Low Temperature Microbial Fuel Cells
title_full Microbial Community and Metabolic Activity in Thiocyanate Degrading Low Temperature Microbial Fuel Cells
title_fullStr Microbial Community and Metabolic Activity in Thiocyanate Degrading Low Temperature Microbial Fuel Cells
title_full_unstemmed Microbial Community and Metabolic Activity in Thiocyanate Degrading Low Temperature Microbial Fuel Cells
title_short Microbial Community and Metabolic Activity in Thiocyanate Degrading Low Temperature Microbial Fuel Cells
title_sort microbial community and metabolic activity in thiocyanate degrading low temperature microbial fuel cells
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172326/
https://www.ncbi.nlm.nih.gov/pubmed/30323799
http://dx.doi.org/10.3389/fmicb.2018.02308
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