Cargando…
Streptozotocin-induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization
The present study aimed to investigate the effects of diabetes mellitus (DM) on the generation of experimental corneal neovascularization (CrNV) and choroidal neovascularization (ChNV). Diabetes was induced in mice by intraperitoneal injection of streptozotocin (STZ). Experimental CrNV and ChNV were...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172380/ https://www.ncbi.nlm.nih.gov/pubmed/30221697 http://dx.doi.org/10.3892/mmr.2018.9445 |
_version_ | 1783360935701774336 |
---|---|
author | Liu, Gaoqin Chen, Lei Cai, Qinhua Wu, Hongya Chen, Zhigang Zhang, Xueguang Lu, Peirong |
author_facet | Liu, Gaoqin Chen, Lei Cai, Qinhua Wu, Hongya Chen, Zhigang Zhang, Xueguang Lu, Peirong |
author_sort | Liu, Gaoqin |
collection | PubMed |
description | The present study aimed to investigate the effects of diabetes mellitus (DM) on the generation of experimental corneal neovascularization (CrNV) and choroidal neovascularization (ChNV). Diabetes was induced in mice by intraperitoneal injection of streptozotocin (STZ). Experimental CrNV and ChNV were induced by alkali injury and laser photocoagulation, respectively. CrNV and ChNV were compared between the STZ-induced diabetic mice and control mice two weeks after injury. Relative expression of angiogenic factors was quantified by reverse transcription-quantitative polymerase chain reaction, and progenitor cell or macrophage accumulation in the early phase following injury was examined by flow cytometric analysis. Compared with the alkali-injured normal mice, the alkali-injured diabetic mice (STZ-induced) exhibited no significant difference in CrNV occurrence, whereas the laser-injured diabetic mice exhibited significantly reduced levels of ChNV compared with those of the laser-injured control animals. The laser-induced intrachoroidal mRNA expression levels of angiogenic factors, including vascular endothelial growth factor, hypoxia-induced factor-1α, chemokine (C-C motif) ligand 3, and stromal cell-derived factor-1α, were reduced in the laser-injured diabetic mice when compared with laser-injured control mice. Furthermore, the laser-induced intrachoroidal infiltration of c-Kit(+) progenitor cells was impaired in the laser-injured diabetic mice compared with the laser-injured control mice. Overall, diabetes did not exert a significant effect on the generation of experimental CrNV. However, diabetes reduced laser-induced ChNV through downregulation of intrachoroidal progenitor cell infiltration and angiogenic factor expression. |
format | Online Article Text |
id | pubmed-6172380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-61723802018-10-19 Streptozotocin-induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization Liu, Gaoqin Chen, Lei Cai, Qinhua Wu, Hongya Chen, Zhigang Zhang, Xueguang Lu, Peirong Mol Med Rep Articles The present study aimed to investigate the effects of diabetes mellitus (DM) on the generation of experimental corneal neovascularization (CrNV) and choroidal neovascularization (ChNV). Diabetes was induced in mice by intraperitoneal injection of streptozotocin (STZ). Experimental CrNV and ChNV were induced by alkali injury and laser photocoagulation, respectively. CrNV and ChNV were compared between the STZ-induced diabetic mice and control mice two weeks after injury. Relative expression of angiogenic factors was quantified by reverse transcription-quantitative polymerase chain reaction, and progenitor cell or macrophage accumulation in the early phase following injury was examined by flow cytometric analysis. Compared with the alkali-injured normal mice, the alkali-injured diabetic mice (STZ-induced) exhibited no significant difference in CrNV occurrence, whereas the laser-injured diabetic mice exhibited significantly reduced levels of ChNV compared with those of the laser-injured control animals. The laser-induced intrachoroidal mRNA expression levels of angiogenic factors, including vascular endothelial growth factor, hypoxia-induced factor-1α, chemokine (C-C motif) ligand 3, and stromal cell-derived factor-1α, were reduced in the laser-injured diabetic mice when compared with laser-injured control mice. Furthermore, the laser-induced intrachoroidal infiltration of c-Kit(+) progenitor cells was impaired in the laser-injured diabetic mice compared with the laser-injured control mice. Overall, diabetes did not exert a significant effect on the generation of experimental CrNV. However, diabetes reduced laser-induced ChNV through downregulation of intrachoroidal progenitor cell infiltration and angiogenic factor expression. D.A. Spandidos 2018-11 2018-09-03 /pmc/articles/PMC6172380/ /pubmed/30221697 http://dx.doi.org/10.3892/mmr.2018.9445 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Gaoqin Chen, Lei Cai, Qinhua Wu, Hongya Chen, Zhigang Zhang, Xueguang Lu, Peirong Streptozotocin-induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization |
title | Streptozotocin-induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization |
title_full | Streptozotocin-induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization |
title_fullStr | Streptozotocin-induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization |
title_full_unstemmed | Streptozotocin-induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization |
title_short | Streptozotocin-induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization |
title_sort | streptozotocin-induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172380/ https://www.ncbi.nlm.nih.gov/pubmed/30221697 http://dx.doi.org/10.3892/mmr.2018.9445 |
work_keys_str_mv | AT liugaoqin streptozotocininduceddiabeticmiceexhibitreducedexperimentalchoroidalneovascularizationbutnotcornealneovascularization AT chenlei streptozotocininduceddiabeticmiceexhibitreducedexperimentalchoroidalneovascularizationbutnotcornealneovascularization AT caiqinhua streptozotocininduceddiabeticmiceexhibitreducedexperimentalchoroidalneovascularizationbutnotcornealneovascularization AT wuhongya streptozotocininduceddiabeticmiceexhibitreducedexperimentalchoroidalneovascularizationbutnotcornealneovascularization AT chenzhigang streptozotocininduceddiabeticmiceexhibitreducedexperimentalchoroidalneovascularizationbutnotcornealneovascularization AT zhangxueguang streptozotocininduceddiabeticmiceexhibitreducedexperimentalchoroidalneovascularizationbutnotcornealneovascularization AT lupeirong streptozotocininduceddiabeticmiceexhibitreducedexperimentalchoroidalneovascularizationbutnotcornealneovascularization |