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Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis

The present study aimed to investigate the effect of chemokine CC ligand 2 (CCL2) on α-synuclein-mediated microglia proliferation and neuronal apoptosis. Primary cultured microglia and primary neurons were isolated and cultured in vitro. Microglia were divided into four groups: The cells in the cont...

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Autores principales: Zhang, Lijun, Ma, Pengju, Guan, Qingkai, Meng, Lei, Su, Linlin, Wang, Lina, Yuan, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172395/
https://www.ncbi.nlm.nih.gov/pubmed/30221727
http://dx.doi.org/10.3892/mmr.2018.9468
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author Zhang, Lijun
Ma, Pengju
Guan, Qingkai
Meng, Lei
Su, Linlin
Wang, Lina
Yuan, Bin
author_facet Zhang, Lijun
Ma, Pengju
Guan, Qingkai
Meng, Lei
Su, Linlin
Wang, Lina
Yuan, Bin
author_sort Zhang, Lijun
collection PubMed
description The present study aimed to investigate the effect of chemokine CC ligand 2 (CCL2) on α-synuclein-mediated microglia proliferation and neuronal apoptosis. Primary cultured microglia and primary neurons were isolated and cultured in vitro. Microglia were divided into four groups: The cells in the control group were treated with an identical amount of PBS, whereas the cells in the CCL2 group were cultured in medium containing 0.05 ng/µl CCL2; cells in the α-synuclein group were treated with medium containing 0.2 ng/µl α-synuclein; and cells in the CCL2 plus α-synuclein group were cultured in medium containing 0.05 ng/µl CCL2 and 0.2 ng/µl α-synuclein. After incubation for 24 h, the proliferation of glial cells, and the level of α-synuclein in the cells, were measured. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and nitric oxide (NO) in the culture medium were also measured. Levels of cleaved caspase-3, Akt and phosphorylated (p)-Akt in neurons treated with primary microglia culture medium in each group were subsequently monitored. The proliferation activity and secretion of TNF-α, IL-1β and NO in the CCL2, α-synuclein and CCL2 plus α-synuclein groups were significantly higher compared with that in the control group (P<0.05), as were the levels of α-synuclein (P<0.01). The levels of neuronal apoptosis and cleaved caspase-3 protein in the CCL2, α-synuclein and CCL2 plus α-synuclein groups were also significantly higher compared with that in the control group (P<0.01). Taken together, these results have demonstrated that CCL2 is able to promote α-synuclein secretion and the apoptosis of neurons induced by α-synuclein, thus inducing proliferation of the microglia and secretion of TNF-α, IL-1β and NO.
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spelling pubmed-61723952018-10-19 Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis Zhang, Lijun Ma, Pengju Guan, Qingkai Meng, Lei Su, Linlin Wang, Lina Yuan, Bin Mol Med Rep Articles The present study aimed to investigate the effect of chemokine CC ligand 2 (CCL2) on α-synuclein-mediated microglia proliferation and neuronal apoptosis. Primary cultured microglia and primary neurons were isolated and cultured in vitro. Microglia were divided into four groups: The cells in the control group were treated with an identical amount of PBS, whereas the cells in the CCL2 group were cultured in medium containing 0.05 ng/µl CCL2; cells in the α-synuclein group were treated with medium containing 0.2 ng/µl α-synuclein; and cells in the CCL2 plus α-synuclein group were cultured in medium containing 0.05 ng/µl CCL2 and 0.2 ng/µl α-synuclein. After incubation for 24 h, the proliferation of glial cells, and the level of α-synuclein in the cells, were measured. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and nitric oxide (NO) in the culture medium were also measured. Levels of cleaved caspase-3, Akt and phosphorylated (p)-Akt in neurons treated with primary microglia culture medium in each group were subsequently monitored. The proliferation activity and secretion of TNF-α, IL-1β and NO in the CCL2, α-synuclein and CCL2 plus α-synuclein groups were significantly higher compared with that in the control group (P<0.05), as were the levels of α-synuclein (P<0.01). The levels of neuronal apoptosis and cleaved caspase-3 protein in the CCL2, α-synuclein and CCL2 plus α-synuclein groups were also significantly higher compared with that in the control group (P<0.01). Taken together, these results have demonstrated that CCL2 is able to promote α-synuclein secretion and the apoptosis of neurons induced by α-synuclein, thus inducing proliferation of the microglia and secretion of TNF-α, IL-1β and NO. D.A. Spandidos 2018-11 2018-09-10 /pmc/articles/PMC6172395/ /pubmed/30221727 http://dx.doi.org/10.3892/mmr.2018.9468 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Lijun
Ma, Pengju
Guan, Qingkai
Meng, Lei
Su, Linlin
Wang, Lina
Yuan, Bin
Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis
title Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis
title_full Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis
title_fullStr Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis
title_full_unstemmed Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis
title_short Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis
title_sort effect of chemokine cc ligand 2 (ccl2) on α-synuclein-induced microglia proliferation and neuronal apoptosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172395/
https://www.ncbi.nlm.nih.gov/pubmed/30221727
http://dx.doi.org/10.3892/mmr.2018.9468
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