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Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis
The present study aimed to investigate the effect of chemokine CC ligand 2 (CCL2) on α-synuclein-mediated microglia proliferation and neuronal apoptosis. Primary cultured microglia and primary neurons were isolated and cultured in vitro. Microglia were divided into four groups: The cells in the cont...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172395/ https://www.ncbi.nlm.nih.gov/pubmed/30221727 http://dx.doi.org/10.3892/mmr.2018.9468 |
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author | Zhang, Lijun Ma, Pengju Guan, Qingkai Meng, Lei Su, Linlin Wang, Lina Yuan, Bin |
author_facet | Zhang, Lijun Ma, Pengju Guan, Qingkai Meng, Lei Su, Linlin Wang, Lina Yuan, Bin |
author_sort | Zhang, Lijun |
collection | PubMed |
description | The present study aimed to investigate the effect of chemokine CC ligand 2 (CCL2) on α-synuclein-mediated microglia proliferation and neuronal apoptosis. Primary cultured microglia and primary neurons were isolated and cultured in vitro. Microglia were divided into four groups: The cells in the control group were treated with an identical amount of PBS, whereas the cells in the CCL2 group were cultured in medium containing 0.05 ng/µl CCL2; cells in the α-synuclein group were treated with medium containing 0.2 ng/µl α-synuclein; and cells in the CCL2 plus α-synuclein group were cultured in medium containing 0.05 ng/µl CCL2 and 0.2 ng/µl α-synuclein. After incubation for 24 h, the proliferation of glial cells, and the level of α-synuclein in the cells, were measured. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and nitric oxide (NO) in the culture medium were also measured. Levels of cleaved caspase-3, Akt and phosphorylated (p)-Akt in neurons treated with primary microglia culture medium in each group were subsequently monitored. The proliferation activity and secretion of TNF-α, IL-1β and NO in the CCL2, α-synuclein and CCL2 plus α-synuclein groups were significantly higher compared with that in the control group (P<0.05), as were the levels of α-synuclein (P<0.01). The levels of neuronal apoptosis and cleaved caspase-3 protein in the CCL2, α-synuclein and CCL2 plus α-synuclein groups were also significantly higher compared with that in the control group (P<0.01). Taken together, these results have demonstrated that CCL2 is able to promote α-synuclein secretion and the apoptosis of neurons induced by α-synuclein, thus inducing proliferation of the microglia and secretion of TNF-α, IL-1β and NO. |
format | Online Article Text |
id | pubmed-6172395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-61723952018-10-19 Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis Zhang, Lijun Ma, Pengju Guan, Qingkai Meng, Lei Su, Linlin Wang, Lina Yuan, Bin Mol Med Rep Articles The present study aimed to investigate the effect of chemokine CC ligand 2 (CCL2) on α-synuclein-mediated microglia proliferation and neuronal apoptosis. Primary cultured microglia and primary neurons were isolated and cultured in vitro. Microglia were divided into four groups: The cells in the control group were treated with an identical amount of PBS, whereas the cells in the CCL2 group were cultured in medium containing 0.05 ng/µl CCL2; cells in the α-synuclein group were treated with medium containing 0.2 ng/µl α-synuclein; and cells in the CCL2 plus α-synuclein group were cultured in medium containing 0.05 ng/µl CCL2 and 0.2 ng/µl α-synuclein. After incubation for 24 h, the proliferation of glial cells, and the level of α-synuclein in the cells, were measured. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and nitric oxide (NO) in the culture medium were also measured. Levels of cleaved caspase-3, Akt and phosphorylated (p)-Akt in neurons treated with primary microglia culture medium in each group were subsequently monitored. The proliferation activity and secretion of TNF-α, IL-1β and NO in the CCL2, α-synuclein and CCL2 plus α-synuclein groups were significantly higher compared with that in the control group (P<0.05), as were the levels of α-synuclein (P<0.01). The levels of neuronal apoptosis and cleaved caspase-3 protein in the CCL2, α-synuclein and CCL2 plus α-synuclein groups were also significantly higher compared with that in the control group (P<0.01). Taken together, these results have demonstrated that CCL2 is able to promote α-synuclein secretion and the apoptosis of neurons induced by α-synuclein, thus inducing proliferation of the microglia and secretion of TNF-α, IL-1β and NO. D.A. Spandidos 2018-11 2018-09-10 /pmc/articles/PMC6172395/ /pubmed/30221727 http://dx.doi.org/10.3892/mmr.2018.9468 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhang, Lijun Ma, Pengju Guan, Qingkai Meng, Lei Su, Linlin Wang, Lina Yuan, Bin Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis |
title | Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis |
title_full | Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis |
title_fullStr | Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis |
title_full_unstemmed | Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis |
title_short | Effect of chemokine CC ligand 2 (CCL2) on α-synuclein-induced microglia proliferation and neuronal apoptosis |
title_sort | effect of chemokine cc ligand 2 (ccl2) on α-synuclein-induced microglia proliferation and neuronal apoptosis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172395/ https://www.ncbi.nlm.nih.gov/pubmed/30221727 http://dx.doi.org/10.3892/mmr.2018.9468 |
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