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Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test
Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody tit...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172408/ https://www.ncbi.nlm.nih.gov/pubmed/30483459 http://dx.doi.org/10.4314/ovj.v8i3.15 |
Sumario: | Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody titre (T(Ab)) respectively. The authors have demonstrated that a positive correlation exists between N.I. and T(Ab) values, this study aimed to add more evidence to this correlation by establishing a graph and its mathematical equations. We found that: N.I. = (1.489 Log T(Ab)) + 1.331; this serves as a base to calculate N.I. using T(Ab) values measured by beta-VNT without going through alpha-VNT and vice versa. At the end of this study, we evaluated the equation accuracy by two parameters; the deviation (d) and the error percentage, which were d = 0.2 and error (%) = 8%, respectively. |
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