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Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test
Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody tit...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172408/ https://www.ncbi.nlm.nih.gov/pubmed/30483459 http://dx.doi.org/10.4314/ovj.v8i3.15 |
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author | Hamidouche, Mohamed Belmessabih, Nassira Boubguira, Abderrahmane Benfenatki, Amina Saada, Naim Sail, Amina Bacha, Fatima |
author_facet | Hamidouche, Mohamed Belmessabih, Nassira Boubguira, Abderrahmane Benfenatki, Amina Saada, Naim Sail, Amina Bacha, Fatima |
author_sort | Hamidouche, Mohamed |
collection | PubMed |
description | Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody titre (T(Ab)) respectively. The authors have demonstrated that a positive correlation exists between N.I. and T(Ab) values, this study aimed to add more evidence to this correlation by establishing a graph and its mathematical equations. We found that: N.I. = (1.489 Log T(Ab)) + 1.331; this serves as a base to calculate N.I. using T(Ab) values measured by beta-VNT without going through alpha-VNT and vice versa. At the end of this study, we evaluated the equation accuracy by two parameters; the deviation (d) and the error percentage, which were d = 0.2 and error (%) = 8%, respectively. |
format | Online Article Text |
id | pubmed-6172408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology |
record_format | MEDLINE/PubMed |
spelling | pubmed-61724082018-11-27 Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test Hamidouche, Mohamed Belmessabih, Nassira Boubguira, Abderrahmane Benfenatki, Amina Saada, Naim Sail, Amina Bacha, Fatima Open Vet J Original Article Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody titre (T(Ab)) respectively. The authors have demonstrated that a positive correlation exists between N.I. and T(Ab) values, this study aimed to add more evidence to this correlation by establishing a graph and its mathematical equations. We found that: N.I. = (1.489 Log T(Ab)) + 1.331; this serves as a base to calculate N.I. using T(Ab) values measured by beta-VNT without going through alpha-VNT and vice versa. At the end of this study, we evaluated the equation accuracy by two parameters; the deviation (d) and the error percentage, which were d = 0.2 and error (%) = 8%, respectively. Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2018 2018-09-29 /pmc/articles/PMC6172408/ /pubmed/30483459 http://dx.doi.org/10.4314/ovj.v8i3.15 Text en Copyright: © Open Veterinary Journal http://creativecommons.org/licenses/by-nc-sa/4.0 Open Veterinary Journal is licensed under a Creative Commons Attribution 4.0 International License. |
spellingShingle | Original Article Hamidouche, Mohamed Belmessabih, Nassira Boubguira, Abderrahmane Benfenatki, Amina Saada, Naim Sail, Amina Bacha, Fatima Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test |
title | Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test |
title_full | Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test |
title_fullStr | Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test |
title_full_unstemmed | Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test |
title_short | Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test |
title_sort | capripoxvirus antibodies detection: relationship between the two methods alpha and beta of virus neutralisation test |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172408/ https://www.ncbi.nlm.nih.gov/pubmed/30483459 http://dx.doi.org/10.4314/ovj.v8i3.15 |
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