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Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test

Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody tit...

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Autores principales: Hamidouche, Mohamed, Belmessabih, Nassira, Boubguira, Abderrahmane, Benfenatki, Amina, Saada, Naim, Sail, Amina, Bacha, Fatima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172408/
https://www.ncbi.nlm.nih.gov/pubmed/30483459
http://dx.doi.org/10.4314/ovj.v8i3.15
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author Hamidouche, Mohamed
Belmessabih, Nassira
Boubguira, Abderrahmane
Benfenatki, Amina
Saada, Naim
Sail, Amina
Bacha, Fatima
author_facet Hamidouche, Mohamed
Belmessabih, Nassira
Boubguira, Abderrahmane
Benfenatki, Amina
Saada, Naim
Sail, Amina
Bacha, Fatima
author_sort Hamidouche, Mohamed
collection PubMed
description Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody titre (T(Ab)) respectively. The authors have demonstrated that a positive correlation exists between N.I. and T(Ab) values, this study aimed to add more evidence to this correlation by establishing a graph and its mathematical equations. We found that: N.I. = (1.489 Log T(Ab)) + 1.331; this serves as a base to calculate N.I. using T(Ab) values measured by beta-VNT without going through alpha-VNT and vice versa. At the end of this study, we evaluated the equation accuracy by two parameters; the deviation (d) and the error percentage, which were d = 0.2 and error (%) = 8%, respectively.
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spelling pubmed-61724082018-11-27 Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test Hamidouche, Mohamed Belmessabih, Nassira Boubguira, Abderrahmane Benfenatki, Amina Saada, Naim Sail, Amina Bacha, Fatima Open Vet J Original Article Virus neutralisation test (VNT) of capripoxvirus (CaPVs) was studied to assess the post-vaccination (vaccine effectiveness) or post-infection antibodies level using two methods: alpha-VNT and beta-VNT which are generally carried out to measure the Neutralising Index (N.I.) and the serum Antibody titre (T(Ab)) respectively. The authors have demonstrated that a positive correlation exists between N.I. and T(Ab) values, this study aimed to add more evidence to this correlation by establishing a graph and its mathematical equations. We found that: N.I. = (1.489 Log T(Ab)) + 1.331; this serves as a base to calculate N.I. using T(Ab) values measured by beta-VNT without going through alpha-VNT and vice versa. At the end of this study, we evaluated the equation accuracy by two parameters; the deviation (d) and the error percentage, which were d = 0.2 and error (%) = 8%, respectively. Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2018 2018-09-29 /pmc/articles/PMC6172408/ /pubmed/30483459 http://dx.doi.org/10.4314/ovj.v8i3.15 Text en Copyright: © Open Veterinary Journal http://creativecommons.org/licenses/by-nc-sa/4.0 Open Veterinary Journal is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Original Article
Hamidouche, Mohamed
Belmessabih, Nassira
Boubguira, Abderrahmane
Benfenatki, Amina
Saada, Naim
Sail, Amina
Bacha, Fatima
Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test
title Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test
title_full Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test
title_fullStr Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test
title_full_unstemmed Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test
title_short Capripoxvirus antibodies detection: Relationship between the two methods alpha and beta of virus neutralisation test
title_sort capripoxvirus antibodies detection: relationship between the two methods alpha and beta of virus neutralisation test
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172408/
https://www.ncbi.nlm.nih.gov/pubmed/30483459
http://dx.doi.org/10.4314/ovj.v8i3.15
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