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Sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of Grp78
Purpose: This article aimed to study the role of sevoflurane pre-conditioning in hepatic ischemia–reperfusion and its potential mechanism. Methods: Rat liver ischemia–reperfusion model was constructed. Serum TNF-α, IL-1β, IL-10, and IL-6 concentrations were detected by ELISA. Malondialdehyde (MDA),...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172422/ https://www.ncbi.nlm.nih.gov/pubmed/30217942 http://dx.doi.org/10.1042/BSR20180549 |
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author | Liu, Di Jin, Xin Zhang, Chunqi Shang, You |
author_facet | Liu, Di Jin, Xin Zhang, Chunqi Shang, You |
author_sort | Liu, Di |
collection | PubMed |
description | Purpose: This article aimed to study the role of sevoflurane pre-conditioning in hepatic ischemia–reperfusion and its potential mechanism. Methods: Rat liver ischemia–reperfusion model was constructed. Serum TNF-α, IL-1β, IL-10, and IL-6 concentrations were detected by ELISA. Malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide (NO) in liver homogenate were determined. Hematoxylin–Eosin (HE) staining, Tunel, and immunohistochemistry were performed. Ischemia–reperfusion hepatocyte model was established. Cells transfection was conducted. Apoptosis was observed by flow cytometry. Quantitative real-time PCR (qRT-PCR) and Western blotting analysis were used. Results: Compared with I/R group, liver damage degree, liver cell apoptosis, and glucose regulatory protein 78 (Grp78) expression was obviously reduced in rats of SEV group. TNF-α, IL-1β, and IL-6 concentrations were also significantly increased (P<0.01). MDA and NO concentrations were dramatically lower (P<0.01) and SOD concentration was significantly higher (P<0.01). Apoptosis rate, Grp78, PERK, eIF2α, and p-c-JNK/JNK expression was also significantly decreased (P<0.01). Sevoflurane significantly reduced apoptosis and expression of PERK, eIF2α, p-c-JNK/JNK by inhibiting the expression of Grp78 (P<0.01). Conclusion: Sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of Grp78. |
format | Online Article Text |
id | pubmed-6172422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61724222018-10-19 Sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of Grp78 Liu, Di Jin, Xin Zhang, Chunqi Shang, You Biosci Rep Research Articles Purpose: This article aimed to study the role of sevoflurane pre-conditioning in hepatic ischemia–reperfusion and its potential mechanism. Methods: Rat liver ischemia–reperfusion model was constructed. Serum TNF-α, IL-1β, IL-10, and IL-6 concentrations were detected by ELISA. Malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide (NO) in liver homogenate were determined. Hematoxylin–Eosin (HE) staining, Tunel, and immunohistochemistry were performed. Ischemia–reperfusion hepatocyte model was established. Cells transfection was conducted. Apoptosis was observed by flow cytometry. Quantitative real-time PCR (qRT-PCR) and Western blotting analysis were used. Results: Compared with I/R group, liver damage degree, liver cell apoptosis, and glucose regulatory protein 78 (Grp78) expression was obviously reduced in rats of SEV group. TNF-α, IL-1β, and IL-6 concentrations were also significantly increased (P<0.01). MDA and NO concentrations were dramatically lower (P<0.01) and SOD concentration was significantly higher (P<0.01). Apoptosis rate, Grp78, PERK, eIF2α, and p-c-JNK/JNK expression was also significantly decreased (P<0.01). Sevoflurane significantly reduced apoptosis and expression of PERK, eIF2α, p-c-JNK/JNK by inhibiting the expression of Grp78 (P<0.01). Conclusion: Sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of Grp78. Portland Press Ltd. 2018-10-05 /pmc/articles/PMC6172422/ /pubmed/30217942 http://dx.doi.org/10.1042/BSR20180549 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Liu, Di Jin, Xin Zhang, Chunqi Shang, You Sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of Grp78 |
title | Sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of Grp78 |
title_full | Sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of Grp78 |
title_fullStr | Sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of Grp78 |
title_full_unstemmed | Sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of Grp78 |
title_short | Sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of Grp78 |
title_sort | sevoflurane relieves hepatic ischemia–reperfusion injury by inhibiting the expression of grp78 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172422/ https://www.ncbi.nlm.nih.gov/pubmed/30217942 http://dx.doi.org/10.1042/BSR20180549 |
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