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Application of black phosphorus nanodots to live cell imaging

BACKGROUND: Black phosphorus (BP) has emerged as a novel class of nanomaterials owing to its unique optical and electronic properties. BP, a two-dimensional (2D) nanomaterial, is a structure where phosphorenes are stacked together in layers by van der Waals interactions. However, although BP nanodot...

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Autores principales: Shin, Yong Cheol, Song, Su-Jin, Lee, Yu Bin, Kang, Moon Sung, Lee, Hyun Uk, Oh, Jin-Woo, Han, Dong-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172722/
https://www.ncbi.nlm.nih.gov/pubmed/30305920
http://dx.doi.org/10.1186/s40824-018-0142-x
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author Shin, Yong Cheol
Song, Su-Jin
Lee, Yu Bin
Kang, Moon Sung
Lee, Hyun Uk
Oh, Jin-Woo
Han, Dong-Wook
author_facet Shin, Yong Cheol
Song, Su-Jin
Lee, Yu Bin
Kang, Moon Sung
Lee, Hyun Uk
Oh, Jin-Woo
Han, Dong-Wook
author_sort Shin, Yong Cheol
collection PubMed
description BACKGROUND: Black phosphorus (BP) has emerged as a novel class of nanomaterials owing to its unique optical and electronic properties. BP, a two-dimensional (2D) nanomaterial, is a structure where phosphorenes are stacked together in layers by van der Waals interactions. However, although BP nanodots have many advantages, their biosafety and biological effect have not yet been elucidated as compared to the other nanomaterials. Therefore, it is particularly important to assess the cytotoxicity of BP nanodots for exploring their potentials as novel biomaterials. METHODS: BP nanodots were prepared by exfoliation with a modified ultrasonication-assisted solution method. The physicochemical properties of BP nanodots were characterized by transmission electron microscopy, dynamic light scattering, Raman spectroscopy, and X-ray diffractometry. In addition, the cytotoxicity of BP nanodots against C2C12 myoblasts was evaluated. Moreover, their cell imaging potential was investigated. RESULTS: Herein, we concentrated on evaluating the cytotoxicity of BP nanodots and investigating their cell imaging potential. It was revealed that the BP nanodots were cytocompatible at a low concentration, although the cell viability was decreased with increasing BP nanodot concentration. Furthermore, our results demonstrated that the cells took up the BP nanodots, and the BP nanodots exhibited green fluorescence. CONCLUSIONS: In conclusion, our findings suggest that the BP nanodots have suitable biocompatibility, and are promising candidates as fluorescence probes for biomedical imaging applications.
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spelling pubmed-61727222018-10-10 Application of black phosphorus nanodots to live cell imaging Shin, Yong Cheol Song, Su-Jin Lee, Yu Bin Kang, Moon Sung Lee, Hyun Uk Oh, Jin-Woo Han, Dong-Wook Biomater Res Short Report BACKGROUND: Black phosphorus (BP) has emerged as a novel class of nanomaterials owing to its unique optical and electronic properties. BP, a two-dimensional (2D) nanomaterial, is a structure where phosphorenes are stacked together in layers by van der Waals interactions. However, although BP nanodots have many advantages, their biosafety and biological effect have not yet been elucidated as compared to the other nanomaterials. Therefore, it is particularly important to assess the cytotoxicity of BP nanodots for exploring their potentials as novel biomaterials. METHODS: BP nanodots were prepared by exfoliation with a modified ultrasonication-assisted solution method. The physicochemical properties of BP nanodots were characterized by transmission electron microscopy, dynamic light scattering, Raman spectroscopy, and X-ray diffractometry. In addition, the cytotoxicity of BP nanodots against C2C12 myoblasts was evaluated. Moreover, their cell imaging potential was investigated. RESULTS: Herein, we concentrated on evaluating the cytotoxicity of BP nanodots and investigating their cell imaging potential. It was revealed that the BP nanodots were cytocompatible at a low concentration, although the cell viability was decreased with increasing BP nanodot concentration. Furthermore, our results demonstrated that the cells took up the BP nanodots, and the BP nanodots exhibited green fluorescence. CONCLUSIONS: In conclusion, our findings suggest that the BP nanodots have suitable biocompatibility, and are promising candidates as fluorescence probes for biomedical imaging applications. BioMed Central 2018-10-04 /pmc/articles/PMC6172722/ /pubmed/30305920 http://dx.doi.org/10.1186/s40824-018-0142-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Shin, Yong Cheol
Song, Su-Jin
Lee, Yu Bin
Kang, Moon Sung
Lee, Hyun Uk
Oh, Jin-Woo
Han, Dong-Wook
Application of black phosphorus nanodots to live cell imaging
title Application of black phosphorus nanodots to live cell imaging
title_full Application of black phosphorus nanodots to live cell imaging
title_fullStr Application of black phosphorus nanodots to live cell imaging
title_full_unstemmed Application of black phosphorus nanodots to live cell imaging
title_short Application of black phosphorus nanodots to live cell imaging
title_sort application of black phosphorus nanodots to live cell imaging
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172722/
https://www.ncbi.nlm.nih.gov/pubmed/30305920
http://dx.doi.org/10.1186/s40824-018-0142-x
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