Lessons from a comparison of immuno-chromatographic and chemiluminescent micro-particle immunoassay in the diagnosis of syphilis

OBJECTIVE: To synthesize lessons from comparison of results obtained from the immuno-chromatographic SD Bioline testing method and the chemiluminescent micro-particle immunoassay Architect in the diagnosis of syphilis at Livingstone Central hospital laboratory. RESULTS: The specificity and sensitivi...

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Autores principales: Masenga, Sepiso K., Mweemba, Mercy, Kachele, Annie, Chalubemba, Yahns, Toloka, Paul, Hamooya, Benson M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172779/
https://www.ncbi.nlm.nih.gov/pubmed/30286790
http://dx.doi.org/10.1186/s13104-018-3808-5
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author Masenga, Sepiso K.
Mweemba, Mercy
Kachele, Annie
Chalubemba, Yahns
Toloka, Paul
Hamooya, Benson M.
author_facet Masenga, Sepiso K.
Mweemba, Mercy
Kachele, Annie
Chalubemba, Yahns
Toloka, Paul
Hamooya, Benson M.
author_sort Masenga, Sepiso K.
collection PubMed
description OBJECTIVE: To synthesize lessons from comparison of results obtained from the immuno-chromatographic SD Bioline testing method and the chemiluminescent micro-particle immunoassay Architect in the diagnosis of syphilis at Livingstone Central hospital laboratory. RESULTS: The specificity and sensitivity of SD Bioline syphilis 3.0 against the chemiluminescent immunoassay using the Architect syphilis Treponema pallidum (TP) was 85.3% and 91.3% respectively with substantial agreement between the two test methods (88%, ĸ  = 0.76; p < 0.0005). We recommend further comprehensive study with a larger sample size and clinical details to ascertain the validity of our findings. We also recommend using a non-treponemal test with the current treponemal tests being used to aid diagnosis.
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spelling pubmed-61727792018-10-15 Lessons from a comparison of immuno-chromatographic and chemiluminescent micro-particle immunoassay in the diagnosis of syphilis Masenga, Sepiso K. Mweemba, Mercy Kachele, Annie Chalubemba, Yahns Toloka, Paul Hamooya, Benson M. BMC Res Notes Research Note OBJECTIVE: To synthesize lessons from comparison of results obtained from the immuno-chromatographic SD Bioline testing method and the chemiluminescent micro-particle immunoassay Architect in the diagnosis of syphilis at Livingstone Central hospital laboratory. RESULTS: The specificity and sensitivity of SD Bioline syphilis 3.0 against the chemiluminescent immunoassay using the Architect syphilis Treponema pallidum (TP) was 85.3% and 91.3% respectively with substantial agreement between the two test methods (88%, ĸ  = 0.76; p < 0.0005). We recommend further comprehensive study with a larger sample size and clinical details to ascertain the validity of our findings. We also recommend using a non-treponemal test with the current treponemal tests being used to aid diagnosis. BioMed Central 2018-10-04 /pmc/articles/PMC6172779/ /pubmed/30286790 http://dx.doi.org/10.1186/s13104-018-3808-5 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Masenga, Sepiso K.
Mweemba, Mercy
Kachele, Annie
Chalubemba, Yahns
Toloka, Paul
Hamooya, Benson M.
Lessons from a comparison of immuno-chromatographic and chemiluminescent micro-particle immunoassay in the diagnosis of syphilis
title Lessons from a comparison of immuno-chromatographic and chemiluminescent micro-particle immunoassay in the diagnosis of syphilis
title_full Lessons from a comparison of immuno-chromatographic and chemiluminescent micro-particle immunoassay in the diagnosis of syphilis
title_fullStr Lessons from a comparison of immuno-chromatographic and chemiluminescent micro-particle immunoassay in the diagnosis of syphilis
title_full_unstemmed Lessons from a comparison of immuno-chromatographic and chemiluminescent micro-particle immunoassay in the diagnosis of syphilis
title_short Lessons from a comparison of immuno-chromatographic and chemiluminescent micro-particle immunoassay in the diagnosis of syphilis
title_sort lessons from a comparison of immuno-chromatographic and chemiluminescent micro-particle immunoassay in the diagnosis of syphilis
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172779/
https://www.ncbi.nlm.nih.gov/pubmed/30286790
http://dx.doi.org/10.1186/s13104-018-3808-5
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