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Long-Term Effects of Fibrin Conduit with Human Mesenchymal Stem Cells and Immunosuppression after Peripheral Nerve Repair in a Xenogenic Model
INTRODUCTION: Previously we showed that a fibrin glue conduit with human mesenchymal stem cells (hMSCs) and cyclosporine A (CsA) enhanced early nerve regeneration. In this study long term effects of this conduit are investigated. METHODS: In a rat model, the sciatic nerve was repaired with fibrin co...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172997/ https://www.ncbi.nlm.nih.gov/pubmed/32634185 http://dx.doi.org/10.1177/2155179018760327 |
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author | McGrath, Aleksandra M. Brohlin, Maria Wiberg, Rebecca Kingham, Paul J. Novikov, Lev N. Wiberg, Mikael Novikova, Liudmila N. |
author_facet | McGrath, Aleksandra M. Brohlin, Maria Wiberg, Rebecca Kingham, Paul J. Novikov, Lev N. Wiberg, Mikael Novikova, Liudmila N. |
author_sort | McGrath, Aleksandra M. |
collection | PubMed |
description | INTRODUCTION: Previously we showed that a fibrin glue conduit with human mesenchymal stem cells (hMSCs) and cyclosporine A (CsA) enhanced early nerve regeneration. In this study long term effects of this conduit are investigated. METHODS: In a rat model, the sciatic nerve was repaired with fibrin conduit containing fibrin matrix, fibrin conduit containing fibrin matrix with CsA treatment and fibrin conduit containing fibrin matrix with hMSCs and CsA treatment, and also with nerve graft as control. RESULTS: At 12 weeks 34% of motoneurons of the control group regenerated axons through the fibrin conduit. CsA treatment alone or with hMSCs resulted in axon regeneration of 67% and 64% motoneurons respectively. The gastrocnemius muscle weight was reduced in the conduit with fibrin matrix. The treatment with CsA or CsA with hMSCs induced recovery of the muscle weight and size of fast type fibers towards the levels of the nerve graft group. DISCUSSION: The transplantation of hMSCs for peripheral nerve injury should be optimized to demonstrate their beneficial effects. The CsA may have its own effect on nerve regeneration. |
format | Online Article Text |
id | pubmed-6172997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-61729972018-10-09 Long-Term Effects of Fibrin Conduit with Human Mesenchymal Stem Cells and Immunosuppression after Peripheral Nerve Repair in a Xenogenic Model McGrath, Aleksandra M. Brohlin, Maria Wiberg, Rebecca Kingham, Paul J. Novikov, Lev N. Wiberg, Mikael Novikova, Liudmila N. Cell Med Original Article INTRODUCTION: Previously we showed that a fibrin glue conduit with human mesenchymal stem cells (hMSCs) and cyclosporine A (CsA) enhanced early nerve regeneration. In this study long term effects of this conduit are investigated. METHODS: In a rat model, the sciatic nerve was repaired with fibrin conduit containing fibrin matrix, fibrin conduit containing fibrin matrix with CsA treatment and fibrin conduit containing fibrin matrix with hMSCs and CsA treatment, and also with nerve graft as control. RESULTS: At 12 weeks 34% of motoneurons of the control group regenerated axons through the fibrin conduit. CsA treatment alone or with hMSCs resulted in axon regeneration of 67% and 64% motoneurons respectively. The gastrocnemius muscle weight was reduced in the conduit with fibrin matrix. The treatment with CsA or CsA with hMSCs induced recovery of the muscle weight and size of fast type fibers towards the levels of the nerve graft group. DISCUSSION: The transplantation of hMSCs for peripheral nerve injury should be optimized to demonstrate their beneficial effects. The CsA may have its own effect on nerve regeneration. SAGE Publications 2018-05-31 /pmc/articles/PMC6172997/ /pubmed/32634185 http://dx.doi.org/10.1177/2155179018760327 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article McGrath, Aleksandra M. Brohlin, Maria Wiberg, Rebecca Kingham, Paul J. Novikov, Lev N. Wiberg, Mikael Novikova, Liudmila N. Long-Term Effects of Fibrin Conduit with Human Mesenchymal Stem Cells and Immunosuppression after Peripheral Nerve Repair in a Xenogenic Model |
title | Long-Term Effects of Fibrin Conduit with Human Mesenchymal Stem Cells and
Immunosuppression after Peripheral Nerve Repair in a Xenogenic Model |
title_full | Long-Term Effects of Fibrin Conduit with Human Mesenchymal Stem Cells and
Immunosuppression after Peripheral Nerve Repair in a Xenogenic Model |
title_fullStr | Long-Term Effects of Fibrin Conduit with Human Mesenchymal Stem Cells and
Immunosuppression after Peripheral Nerve Repair in a Xenogenic Model |
title_full_unstemmed | Long-Term Effects of Fibrin Conduit with Human Mesenchymal Stem Cells and
Immunosuppression after Peripheral Nerve Repair in a Xenogenic Model |
title_short | Long-Term Effects of Fibrin Conduit with Human Mesenchymal Stem Cells and
Immunosuppression after Peripheral Nerve Repair in a Xenogenic Model |
title_sort | long-term effects of fibrin conduit with human mesenchymal stem cells and
immunosuppression after peripheral nerve repair in a xenogenic model |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172997/ https://www.ncbi.nlm.nih.gov/pubmed/32634185 http://dx.doi.org/10.1177/2155179018760327 |
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