Cargando…

The Development of Cancer through the Transient Overexpression of Reprogramming Factors

Although induced pluripotent stem (iPS) cells have significant implications for overcoming most of the ethical issues associated with embryonic stem cells, several issues related to the use of iPS cells in clinical applications remain unresolved, including the issue of teratoma formation. We previou...

Descripción completa

Detalles Bibliográficos
Autores principales: Miyagi-Shiohira, Chika, Nakashima, Yoshiki, Kobayashi, Naoya, Saitoh, Issei, Watanabe, Masami, Noguchi, Yasufumi, Kinjo, Takao, Noguchi, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172998/
https://www.ncbi.nlm.nih.gov/pubmed/32634181
http://dx.doi.org/10.1177/2155179017733172
_version_ 1783361052041281536
author Miyagi-Shiohira, Chika
Nakashima, Yoshiki
Kobayashi, Naoya
Saitoh, Issei
Watanabe, Masami
Noguchi, Yasufumi
Kinjo, Takao
Noguchi, Hirofumi
author_facet Miyagi-Shiohira, Chika
Nakashima, Yoshiki
Kobayashi, Naoya
Saitoh, Issei
Watanabe, Masami
Noguchi, Yasufumi
Kinjo, Takao
Noguchi, Hirofumi
author_sort Miyagi-Shiohira, Chika
collection PubMed
description Although induced pluripotent stem (iPS) cells have significant implications for overcoming most of the ethical issues associated with embryonic stem cells, several issues related to the use of iPS cells in clinical applications remain unresolved, including the issue of teratoma formation. We previously reported that the induction of induced tissue-specific stem (iTS) cells from the pancreas (iTS-P) or liver (iTS-L) by the transient overexpression of reprogramming factors, combined with tissue-specific selection and the generation of iTS cells, could have important implications for the clinical application of stem cells. At the same time, we also generated “induced fibroblast-like (iF) cells” that were capable of self-renewal, which had a similar morphology to fibroblast cells. In this study, we evaluated iF cells. iF cells are unlikely to show adipogenic/osteogenic differentiation. Moreover, iF cells have the ability to form tumors and behave similarly to pancreatic cancer cells. The technology used in the generation of iPS/iTS cells is also associated with the risk of generating cancer-like cells.
format Online
Article
Text
id pubmed-6172998
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-61729982018-10-09 The Development of Cancer through the Transient Overexpression of Reprogramming Factors Miyagi-Shiohira, Chika Nakashima, Yoshiki Kobayashi, Naoya Saitoh, Issei Watanabe, Masami Noguchi, Yasufumi Kinjo, Takao Noguchi, Hirofumi Cell Med Original Article Although induced pluripotent stem (iPS) cells have significant implications for overcoming most of the ethical issues associated with embryonic stem cells, several issues related to the use of iPS cells in clinical applications remain unresolved, including the issue of teratoma formation. We previously reported that the induction of induced tissue-specific stem (iTS) cells from the pancreas (iTS-P) or liver (iTS-L) by the transient overexpression of reprogramming factors, combined with tissue-specific selection and the generation of iTS cells, could have important implications for the clinical application of stem cells. At the same time, we also generated “induced fibroblast-like (iF) cells” that were capable of self-renewal, which had a similar morphology to fibroblast cells. In this study, we evaluated iF cells. iF cells are unlikely to show adipogenic/osteogenic differentiation. Moreover, iF cells have the ability to form tumors and behave similarly to pancreatic cancer cells. The technology used in the generation of iPS/iTS cells is also associated with the risk of generating cancer-like cells. SAGE Publications 2018-05-29 /pmc/articles/PMC6172998/ /pubmed/32634181 http://dx.doi.org/10.1177/2155179017733172 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Miyagi-Shiohira, Chika
Nakashima, Yoshiki
Kobayashi, Naoya
Saitoh, Issei
Watanabe, Masami
Noguchi, Yasufumi
Kinjo, Takao
Noguchi, Hirofumi
The Development of Cancer through the Transient Overexpression of Reprogramming Factors
title The Development of Cancer through the Transient Overexpression of Reprogramming Factors
title_full The Development of Cancer through the Transient Overexpression of Reprogramming Factors
title_fullStr The Development of Cancer through the Transient Overexpression of Reprogramming Factors
title_full_unstemmed The Development of Cancer through the Transient Overexpression of Reprogramming Factors
title_short The Development of Cancer through the Transient Overexpression of Reprogramming Factors
title_sort development of cancer through the transient overexpression of reprogramming factors
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172998/
https://www.ncbi.nlm.nih.gov/pubmed/32634181
http://dx.doi.org/10.1177/2155179017733172
work_keys_str_mv AT miyagishiohirachika thedevelopmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT nakashimayoshiki thedevelopmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT kobayashinaoya thedevelopmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT saitohissei thedevelopmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT watanabemasami thedevelopmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT noguchiyasufumi thedevelopmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT kinjotakao thedevelopmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT noguchihirofumi thedevelopmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT miyagishiohirachika developmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT nakashimayoshiki developmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT kobayashinaoya developmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT saitohissei developmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT watanabemasami developmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT noguchiyasufumi developmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT kinjotakao developmentofcancerthroughthetransientoverexpressionofreprogrammingfactors
AT noguchihirofumi developmentofcancerthroughthetransientoverexpressionofreprogrammingfactors