Multicolor single particle reconstruction of protein complexes

Single-particle reconstruction (SPR) from electron microscopy images is widely used in structural biology, but lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and co-aligns multiple proteins from 2D sup...

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Detalles Bibliográficos
Autores principales: Sieben, Christian, Banterle, Niccolò, Douglass, Kyle M., Gönczy, Pierre, Manley, Suliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173288/
https://www.ncbi.nlm.nih.gov/pubmed/30275574
http://dx.doi.org/10.1038/s41592-018-0140-x
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author Sieben, Christian
Banterle, Niccolò
Douglass, Kyle M.
Gönczy, Pierre
Manley, Suliana
author_facet Sieben, Christian
Banterle, Niccolò
Douglass, Kyle M.
Gönczy, Pierre
Manley, Suliana
author_sort Sieben, Christian
collection PubMed
description Single-particle reconstruction (SPR) from electron microscopy images is widely used in structural biology, but lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and co-aligns multiple proteins from 2D superresolution fluorescence images. We demonstrate our method by generating multicolor 3D reconstructions of several proteins within the human centriole, revealing their relative locations, dimensions and orientations.
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spelling pubmed-61732882019-04-01 Multicolor single particle reconstruction of protein complexes Sieben, Christian Banterle, Niccolò Douglass, Kyle M. Gönczy, Pierre Manley, Suliana Nat Methods Article Single-particle reconstruction (SPR) from electron microscopy images is widely used in structural biology, but lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and co-aligns multiple proteins from 2D superresolution fluorescence images. We demonstrate our method by generating multicolor 3D reconstructions of several proteins within the human centriole, revealing their relative locations, dimensions and orientations. 2018-10-01 2018-10 /pmc/articles/PMC6173288/ /pubmed/30275574 http://dx.doi.org/10.1038/s41592-018-0140-x Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Sieben, Christian
Banterle, Niccolò
Douglass, Kyle M.
Gönczy, Pierre
Manley, Suliana
Multicolor single particle reconstruction of protein complexes
title Multicolor single particle reconstruction of protein complexes
title_full Multicolor single particle reconstruction of protein complexes
title_fullStr Multicolor single particle reconstruction of protein complexes
title_full_unstemmed Multicolor single particle reconstruction of protein complexes
title_short Multicolor single particle reconstruction of protein complexes
title_sort multicolor single particle reconstruction of protein complexes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173288/
https://www.ncbi.nlm.nih.gov/pubmed/30275574
http://dx.doi.org/10.1038/s41592-018-0140-x
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AT gonczypierre multicolorsingleparticlereconstructionofproteincomplexes
AT manleysuliana multicolorsingleparticlereconstructionofproteincomplexes

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