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Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats

BACKGROUND: Caloric restriction is known to impair the cardiac function and morphology in hypertrophied hearts of spontaneously hypertensive rats (SHR); however, the influence of fasting/refeeding (RF) is unknown. OBJECTIVE: To investigate the fasting/refeeding approach on myocardial remodeling and...

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Autores principales: Pinotti, Matheus Fécchio, Matias, Amanda Martins, Sugizaki, Mário Mateus, do Nascimento, André Ferreira, Pai, Maeli Dal, Leopoldo, Ana Paula Lima, Cicogna, Antônio Carlos, Leopoldo, André Soares
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Cardiologia - SBC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173345/
https://www.ncbi.nlm.nih.gov/pubmed/30133552
http://dx.doi.org/10.5935/abc.20180152
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author Pinotti, Matheus Fécchio
Matias, Amanda Martins
Sugizaki, Mário Mateus
do Nascimento, André Ferreira
Pai, Maeli Dal
Leopoldo, Ana Paula Lima
Cicogna, Antônio Carlos
Leopoldo, André Soares
author_facet Pinotti, Matheus Fécchio
Matias, Amanda Martins
Sugizaki, Mário Mateus
do Nascimento, André Ferreira
Pai, Maeli Dal
Leopoldo, Ana Paula Lima
Cicogna, Antônio Carlos
Leopoldo, André Soares
author_sort Pinotti, Matheus Fécchio
collection PubMed
description BACKGROUND: Caloric restriction is known to impair the cardiac function and morphology in hypertrophied hearts of spontaneously hypertensive rats (SHR); however, the influence of fasting/refeeding (RF) is unknown. OBJECTIVE: To investigate the fasting/refeeding approach on myocardial remodeling and function. In addition, the current study was designed to bring information regarding the mechanisms underlying the participation of Ca(2+) handling and β-adrenergic system. METHODS: Sixty-day-old male SHR rats were submitted to food ad libitum (C), 50% food restriction (R(50)) or RF cycles for 90 days. Cardiac remodeling was assessed by ultrastructure analysis and isolated papillary muscle function. The level of significance considered was 5% (α = 0.05). RESULTS: The RF rats presented lower cardiac atrophy than R(50) in relation to C rats. The C rats increased weight gain, R(50) maintained their initial body weight and RF rats increased and decreased weight during RF. The RF did not cause functional impairment because the isotonic and isometric parameters showed similar behavior to those of C. The isotonic and isometric cardiac parameters were significantly elevated in RF rats compared to R(50) rats. In addition, the R(50) rats had cardiac damage in relation to C for isotonic and isometric variables. While the R(50) rats showed focal changes in many muscle fibers, the RF rats displayed mild alterations, such as loss or disorganization of myofibrils. CONCLUSION: Fasting/refeeding promotes cardiac beneficial effects and attenuates myocardial injury caused by caloric restriction in SHR rats, contributing to reduce the cardiovascular risk profile and morphological injuries. Furthermore, RF promotes mild improvement in Ca(2+) handling and β-adrenergic system.
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spelling pubmed-61733452018-10-10 Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats Pinotti, Matheus Fécchio Matias, Amanda Martins Sugizaki, Mário Mateus do Nascimento, André Ferreira Pai, Maeli Dal Leopoldo, Ana Paula Lima Cicogna, Antônio Carlos Leopoldo, André Soares Arq Bras Cardiol Original Article BACKGROUND: Caloric restriction is known to impair the cardiac function and morphology in hypertrophied hearts of spontaneously hypertensive rats (SHR); however, the influence of fasting/refeeding (RF) is unknown. OBJECTIVE: To investigate the fasting/refeeding approach on myocardial remodeling and function. In addition, the current study was designed to bring information regarding the mechanisms underlying the participation of Ca(2+) handling and β-adrenergic system. METHODS: Sixty-day-old male SHR rats were submitted to food ad libitum (C), 50% food restriction (R(50)) or RF cycles for 90 days. Cardiac remodeling was assessed by ultrastructure analysis and isolated papillary muscle function. The level of significance considered was 5% (α = 0.05). RESULTS: The RF rats presented lower cardiac atrophy than R(50) in relation to C rats. The C rats increased weight gain, R(50) maintained their initial body weight and RF rats increased and decreased weight during RF. The RF did not cause functional impairment because the isotonic and isometric parameters showed similar behavior to those of C. The isotonic and isometric cardiac parameters were significantly elevated in RF rats compared to R(50) rats. In addition, the R(50) rats had cardiac damage in relation to C for isotonic and isometric variables. While the R(50) rats showed focal changes in many muscle fibers, the RF rats displayed mild alterations, such as loss or disorganization of myofibrils. CONCLUSION: Fasting/refeeding promotes cardiac beneficial effects and attenuates myocardial injury caused by caloric restriction in SHR rats, contributing to reduce the cardiovascular risk profile and morphological injuries. Furthermore, RF promotes mild improvement in Ca(2+) handling and β-adrenergic system. Sociedade Brasileira de Cardiologia - SBC 2018-09 /pmc/articles/PMC6173345/ /pubmed/30133552 http://dx.doi.org/10.5935/abc.20180152 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Pinotti, Matheus Fécchio
Matias, Amanda Martins
Sugizaki, Mário Mateus
do Nascimento, André Ferreira
Pai, Maeli Dal
Leopoldo, Ana Paula Lima
Cicogna, Antônio Carlos
Leopoldo, André Soares
Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
title Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
title_full Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
title_fullStr Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
title_full_unstemmed Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
title_short Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
title_sort fasting/refeeding cycles prevent myocardial dysfunction and morphology damage in the spontaneously hypertensive rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173345/
https://www.ncbi.nlm.nih.gov/pubmed/30133552
http://dx.doi.org/10.5935/abc.20180152
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