Label-Free Electrochemical Immunosensor Based on Ionic Liquid Containing Dialdehyde As a Novel Linking Agent for the Antibody Immobilization

[Image: see text] Demand for label-free electrochemical immunosensor has resulted in extensive research in improving the conductivity of a sensing interface and antibody immobilization. In this paper, an electrochemical immunosensor for prostate specific antigen based on dialdehyde-functionalized io...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Youming, Shen, Guangyu, Zhang, Youyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173497/
https://www.ncbi.nlm.nih.gov/pubmed/30320258
http://dx.doi.org/10.1021/acsomega.8b01298
_version_ 1783361143045095424
author Shen, Youming
Shen, Guangyu
Zhang, Youyu
author_facet Shen, Youming
Shen, Guangyu
Zhang, Youyu
author_sort Shen, Youming
collection PubMed
description [Image: see text] Demand for label-free electrochemical immunosensor has resulted in extensive research in improving the conductivity of a sensing interface and antibody immobilization. In this paper, an electrochemical immunosensor for prostate specific antigen based on dialdehyde-functionalized ionic liquid used as a novel linking reagent to replace glutaraldehyde for the antibody immobilization is described. The novel linking reagent enhanced the conductivity of the sensing interface. Thus, the proposed immunosensor had a wider linear range of 0.05–30 ng mL(–1), with a lower detection limit of 0.04 ng mL(–1) compared with the immunosensor based on glutaraldehyde for the antibody immobilization.
format Online
Article
Text
id pubmed-6173497
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-61734972018-10-11 Label-Free Electrochemical Immunosensor Based on Ionic Liquid Containing Dialdehyde As a Novel Linking Agent for the Antibody Immobilization Shen, Youming Shen, Guangyu Zhang, Youyu ACS Omega [Image: see text] Demand for label-free electrochemical immunosensor has resulted in extensive research in improving the conductivity of a sensing interface and antibody immobilization. In this paper, an electrochemical immunosensor for prostate specific antigen based on dialdehyde-functionalized ionic liquid used as a novel linking reagent to replace glutaraldehyde for the antibody immobilization is described. The novel linking reagent enhanced the conductivity of the sensing interface. Thus, the proposed immunosensor had a wider linear range of 0.05–30 ng mL(–1), with a lower detection limit of 0.04 ng mL(–1) compared with the immunosensor based on glutaraldehyde for the antibody immobilization. American Chemical Society 2018-09-14 /pmc/articles/PMC6173497/ /pubmed/30320258 http://dx.doi.org/10.1021/acsomega.8b01298 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Shen, Youming
Shen, Guangyu
Zhang, Youyu
Label-Free Electrochemical Immunosensor Based on Ionic Liquid Containing Dialdehyde As a Novel Linking Agent for the Antibody Immobilization
title Label-Free Electrochemical Immunosensor Based on Ionic Liquid Containing Dialdehyde As a Novel Linking Agent for the Antibody Immobilization
title_full Label-Free Electrochemical Immunosensor Based on Ionic Liquid Containing Dialdehyde As a Novel Linking Agent for the Antibody Immobilization
title_fullStr Label-Free Electrochemical Immunosensor Based on Ionic Liquid Containing Dialdehyde As a Novel Linking Agent for the Antibody Immobilization
title_full_unstemmed Label-Free Electrochemical Immunosensor Based on Ionic Liquid Containing Dialdehyde As a Novel Linking Agent for the Antibody Immobilization
title_short Label-Free Electrochemical Immunosensor Based on Ionic Liquid Containing Dialdehyde As a Novel Linking Agent for the Antibody Immobilization
title_sort label-free electrochemical immunosensor based on ionic liquid containing dialdehyde as a novel linking agent for the antibody immobilization
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173497/
https://www.ncbi.nlm.nih.gov/pubmed/30320258
http://dx.doi.org/10.1021/acsomega.8b01298
work_keys_str_mv AT shenyouming labelfreeelectrochemicalimmunosensorbasedonionicliquidcontainingdialdehydeasanovellinkingagentfortheantibodyimmobilization
AT shenguangyu labelfreeelectrochemicalimmunosensorbasedonionicliquidcontainingdialdehydeasanovellinkingagentfortheantibodyimmobilization
AT zhangyouyu labelfreeelectrochemicalimmunosensorbasedonionicliquidcontainingdialdehydeasanovellinkingagentfortheantibodyimmobilization

Ejemplares similares