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Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A

[Image: see text] Fullerene–protein interaction studies have been a key topic of investigation in recent times, but the lower water solubility of fullerene somewhat limits its application in the biological system. In this work, we have compared the activities of fullerene and its water-soluble hydra...

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Autores principales: Roy, Pritam, Bag, Sudipta, Chakraborty, Debanjana, Dasgupta, Swagata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173555/
https://www.ncbi.nlm.nih.gov/pubmed/30320292
http://dx.doi.org/10.1021/acsomega.8b01584
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author Roy, Pritam
Bag, Sudipta
Chakraborty, Debanjana
Dasgupta, Swagata
author_facet Roy, Pritam
Bag, Sudipta
Chakraborty, Debanjana
Dasgupta, Swagata
author_sort Roy, Pritam
collection PubMed
description [Image: see text] Fullerene–protein interaction studies have been a key topic of investigation in recent times, but the lower water solubility of fullerene somewhat limits its application in the biological system. In this work, we have compared the activities of fullerene and its water-soluble hydrated form, that is fullerenol, on ribonuclease A (RNase A) under physiological conditions (pH 7.4). The interaction studies of fullerene and fullerenol with protein suggest that the binding depends on the hydrophobic interactions between the protein and the ligand. In addition, fullerene and fullerenol slow down the ribonucleolytic activity of RNase A through noncompetitive and mixed types of inhibition, respectively. This precisely gives the idea about the ligand-binding sites in RNase A, which has further been explored using docking studies. Both these nanoparticles show a reduction in dityrosine formation in RNase A caused due to oxidative stress and also prevent RNase A dimer formation to different extents depending on their concentration.
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spelling pubmed-61735552018-10-11 Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A Roy, Pritam Bag, Sudipta Chakraborty, Debanjana Dasgupta, Swagata ACS Omega [Image: see text] Fullerene–protein interaction studies have been a key topic of investigation in recent times, but the lower water solubility of fullerene somewhat limits its application in the biological system. In this work, we have compared the activities of fullerene and its water-soluble hydrated form, that is fullerenol, on ribonuclease A (RNase A) under physiological conditions (pH 7.4). The interaction studies of fullerene and fullerenol with protein suggest that the binding depends on the hydrophobic interactions between the protein and the ligand. In addition, fullerene and fullerenol slow down the ribonucleolytic activity of RNase A through noncompetitive and mixed types of inhibition, respectively. This precisely gives the idea about the ligand-binding sites in RNase A, which has further been explored using docking studies. Both these nanoparticles show a reduction in dityrosine formation in RNase A caused due to oxidative stress and also prevent RNase A dimer formation to different extents depending on their concentration. American Chemical Society 2018-09-28 /pmc/articles/PMC6173555/ /pubmed/30320292 http://dx.doi.org/10.1021/acsomega.8b01584 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Roy, Pritam
Bag, Sudipta
Chakraborty, Debanjana
Dasgupta, Swagata
Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A
title Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A
title_full Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A
title_fullStr Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A
title_full_unstemmed Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A
title_short Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A
title_sort exploring the inhibitory and antioxidant effects of fullerene and fullerenol on ribonuclease a
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173555/
https://www.ncbi.nlm.nih.gov/pubmed/30320292
http://dx.doi.org/10.1021/acsomega.8b01584
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