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Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A
[Image: see text] Fullerene–protein interaction studies have been a key topic of investigation in recent times, but the lower water solubility of fullerene somewhat limits its application in the biological system. In this work, we have compared the activities of fullerene and its water-soluble hydra...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173555/ https://www.ncbi.nlm.nih.gov/pubmed/30320292 http://dx.doi.org/10.1021/acsomega.8b01584 |
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author | Roy, Pritam Bag, Sudipta Chakraborty, Debanjana Dasgupta, Swagata |
author_facet | Roy, Pritam Bag, Sudipta Chakraborty, Debanjana Dasgupta, Swagata |
author_sort | Roy, Pritam |
collection | PubMed |
description | [Image: see text] Fullerene–protein interaction studies have been a key topic of investigation in recent times, but the lower water solubility of fullerene somewhat limits its application in the biological system. In this work, we have compared the activities of fullerene and its water-soluble hydrated form, that is fullerenol, on ribonuclease A (RNase A) under physiological conditions (pH 7.4). The interaction studies of fullerene and fullerenol with protein suggest that the binding depends on the hydrophobic interactions between the protein and the ligand. In addition, fullerene and fullerenol slow down the ribonucleolytic activity of RNase A through noncompetitive and mixed types of inhibition, respectively. This precisely gives the idea about the ligand-binding sites in RNase A, which has further been explored using docking studies. Both these nanoparticles show a reduction in dityrosine formation in RNase A caused due to oxidative stress and also prevent RNase A dimer formation to different extents depending on their concentration. |
format | Online Article Text |
id | pubmed-6173555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-61735552018-10-11 Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A Roy, Pritam Bag, Sudipta Chakraborty, Debanjana Dasgupta, Swagata ACS Omega [Image: see text] Fullerene–protein interaction studies have been a key topic of investigation in recent times, but the lower water solubility of fullerene somewhat limits its application in the biological system. In this work, we have compared the activities of fullerene and its water-soluble hydrated form, that is fullerenol, on ribonuclease A (RNase A) under physiological conditions (pH 7.4). The interaction studies of fullerene and fullerenol with protein suggest that the binding depends on the hydrophobic interactions between the protein and the ligand. In addition, fullerene and fullerenol slow down the ribonucleolytic activity of RNase A through noncompetitive and mixed types of inhibition, respectively. This precisely gives the idea about the ligand-binding sites in RNase A, which has further been explored using docking studies. Both these nanoparticles show a reduction in dityrosine formation in RNase A caused due to oxidative stress and also prevent RNase A dimer formation to different extents depending on their concentration. American Chemical Society 2018-09-28 /pmc/articles/PMC6173555/ /pubmed/30320292 http://dx.doi.org/10.1021/acsomega.8b01584 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Roy, Pritam Bag, Sudipta Chakraborty, Debanjana Dasgupta, Swagata Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol on Ribonuclease A |
title | Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol
on Ribonuclease A |
title_full | Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol
on Ribonuclease A |
title_fullStr | Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol
on Ribonuclease A |
title_full_unstemmed | Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol
on Ribonuclease A |
title_short | Exploring the Inhibitory and Antioxidant Effects of Fullerene and Fullerenol
on Ribonuclease A |
title_sort | exploring the inhibitory and antioxidant effects of fullerene and fullerenol
on ribonuclease a |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173555/ https://www.ncbi.nlm.nih.gov/pubmed/30320292 http://dx.doi.org/10.1021/acsomega.8b01584 |
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