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Self-induced mechanical stress can trigger biofilm formation in uropathogenic Escherichia coli
Bacterial biofilms represent an important medical problem; however, the mechanisms of the onset of biofilm formation are poorly understood. Here, using new controlled methods allowing high-throughput and reproducible biofilm growth, we show that biofilm formation is linked to self-imposed mechanical...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173693/ https://www.ncbi.nlm.nih.gov/pubmed/30291231 http://dx.doi.org/10.1038/s41467-018-06552-z |
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author | Chu, Eric K. Kilic, Onur Cho, Hojung Groisman, Alex Levchenko, Andre |
author_facet | Chu, Eric K. Kilic, Onur Cho, Hojung Groisman, Alex Levchenko, Andre |
author_sort | Chu, Eric K. |
collection | PubMed |
description | Bacterial biofilms represent an important medical problem; however, the mechanisms of the onset of biofilm formation are poorly understood. Here, using new controlled methods allowing high-throughput and reproducible biofilm growth, we show that biofilm formation is linked to self-imposed mechanical stress. In growing uropathogenic Escherichia coli colonies, we report that mechanical stress can initially emerge from the physical stress accompanying colony confinement within micro-cavities or hydrogel environments reminiscent of the cytosol of host cells. Biofilm formation can then be enhanced by a nutrient access-modulated feedback loop, in which biofilm matrix deposition can be particularly high in areas of increased mechanical and biological stress, with the deposited matrix further enhancing the stress levels. This feedback regulation can lead to adaptive and diverse biofilm formation guided by the environmental stresses. Our results suggest previously unappreciated mechanisms of the onset and progression of biofilm growth. |
format | Online Article Text |
id | pubmed-6173693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61736932018-10-09 Self-induced mechanical stress can trigger biofilm formation in uropathogenic Escherichia coli Chu, Eric K. Kilic, Onur Cho, Hojung Groisman, Alex Levchenko, Andre Nat Commun Article Bacterial biofilms represent an important medical problem; however, the mechanisms of the onset of biofilm formation are poorly understood. Here, using new controlled methods allowing high-throughput and reproducible biofilm growth, we show that biofilm formation is linked to self-imposed mechanical stress. In growing uropathogenic Escherichia coli colonies, we report that mechanical stress can initially emerge from the physical stress accompanying colony confinement within micro-cavities or hydrogel environments reminiscent of the cytosol of host cells. Biofilm formation can then be enhanced by a nutrient access-modulated feedback loop, in which biofilm matrix deposition can be particularly high in areas of increased mechanical and biological stress, with the deposited matrix further enhancing the stress levels. This feedback regulation can lead to adaptive and diverse biofilm formation guided by the environmental stresses. Our results suggest previously unappreciated mechanisms of the onset and progression of biofilm growth. Nature Publishing Group UK 2018-10-05 /pmc/articles/PMC6173693/ /pubmed/30291231 http://dx.doi.org/10.1038/s41467-018-06552-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Chu, Eric K. Kilic, Onur Cho, Hojung Groisman, Alex Levchenko, Andre Self-induced mechanical stress can trigger biofilm formation in uropathogenic Escherichia coli |
title | Self-induced mechanical stress can trigger biofilm formation in uropathogenic Escherichia coli |
title_full | Self-induced mechanical stress can trigger biofilm formation in uropathogenic Escherichia coli |
title_fullStr | Self-induced mechanical stress can trigger biofilm formation in uropathogenic Escherichia coli |
title_full_unstemmed | Self-induced mechanical stress can trigger biofilm formation in uropathogenic Escherichia coli |
title_short | Self-induced mechanical stress can trigger biofilm formation in uropathogenic Escherichia coli |
title_sort | self-induced mechanical stress can trigger biofilm formation in uropathogenic escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173693/ https://www.ncbi.nlm.nih.gov/pubmed/30291231 http://dx.doi.org/10.1038/s41467-018-06552-z |
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